##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BciVI BcnI BfrI BglI BlnI
BsaHI BsaI BsiEI BsiWI BsmBI
BspDI BspHI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MfeI
MluI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1100 TCCGGA T^CCGGA
AciI 362 CCGC CCGC(-3/-1)
AclI 255 AACGTT AA^CGTT
AocI 2719 CCTAAGG CC^T[ACGT]AGG
AseI 3014 ATTAAT AT^TAAT
AsnI 3014 ATTAAT AT^TAAT
Asp700I 127 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2183 CCCGAG C^[CT]CG[GA]G
BalI 284 TGGCCA TGG^CCA
BanII 732 GAGCTC G[GA]GC[CT]^C
BbrPI 2779 CACGTG CAC^GTG
BcgI 2091 GCATCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1179 AGATCT A^GATCT
BlpI 2689 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2689 GCTCAGC GC^T[ACGT]AGC
BsaAI 2779 CACGTG [CT]AC^GT[GA]
BsaBI 2843 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1100 TCCGGA [AT]^CCGG[AT]
BseAI 1100 TCCGGA T^CCGGA
BsgI 1301 GTGCAG GTGCAG(16/14)
BsoBI 2183 CCCGAG C^[CT]CG[GA]G
BspEI 1100 TCCGGA T^CCGGA
BspMI 2815 GCAGGT ACCTGC(4/8)
BstAPI 1341 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2719 CCTAAGG CC^T[ACGT]AGG
CelII 2689 GCTCAGC GC^T[ACGT]AGC
DsaI 2194 CCGTGG C^C[GA][CT]GG
EaeI 284 TGGCCA [CT]^GGCC[GA]
Eco81I 2719 CCTAAGG CC^T[ACGT]AGG
HapII 1101 CCGG C^CGG
HincII 2135 GTTAAC GT[CT]^[GA]AC
HindII 2135 GTTAAC GT[CT]^[GA]AC
HpaI 2135 GTTAAC GTT^AAC
HpaII 1101 CCGG C^CGG
MamI 2843 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 284 TGGCCA TGG^CCA
MroI 1100 TCCGGA T^CCGGA
MscI 284 TGGCCA TGG^CCA
MspI 1101 CCGG C^CGG
PleI 664 GAGTC GAGTC(4/5)
PmaCI 2779 CACGTG CAC^GTG
PmlI 2779 CACGTG CAC^GTG
PshAI 369 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3014 ATTAAT AT^TAAT
Psp1406I 255 AACGTT AA^CGTT
SacI 732 GAGCTC GAGCT^C
SapI 841 GAAGAGC GCTCTTC(1/4)
SexAI 1437 ACCAGGT A^CC[AT]GGT
SmlI 472 CTCAAG C^T[CT][GA]AG
XbaI 906 TCTAGA T^CTAGA
XcmI 1871 CCACTCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 127 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1632 GTAGAC GT^[AC][GT]AC
2058 GTAGAC
AlwNI 892 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2082 CAGGAGCTG
AspHI 732 GAGCTC G[AT]GC[AT]^C
2279 GTGCTC
BanI 358 GGTGCC G^G[CT][GA]CC
3080 GGCACC
BclI 744 TGATCA T^GATCA
1932 TGATCA
BmyI 732 GAGCTC G[AGT]GC[ACT]^C
2279 GTGCTC
BsaJI 2184 CCGAGG C^C[ACGT][ACGT]GG
2194 CCGTGG
BsiHKAI 732 GAGCTC G[AT]GC[AT]^C
2279 GTGCTC
BsmFI 294 GGGAC GGGAC(10/14)
2105 GTCCC
BsmI 1885 GAATGC GAATGC(1/-1)
2609 GAATGC
Bsp1286I 732 GAGCTC G[AGT]GC[ACT]^C
2279 GTGCTC
Bsp1407I 278 TGTACA T^GTACA
2356 TGTACA
BspLU11I 1572 ACATGT A^CATGT
2821 ACATGT
BsrGI 278 TGTACA T^GTACA
2356 TGTACA
BssKI 630 CCTGG ^CC[ACGT]GG
1438 CCAGG
BstBI 650 TTCGAA TT^CGAA
1284 TTCGAA
BstNI 630 CCTGG CC^[AT]GG
1438 CCAGG
BstXI 455 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1113 CCACCCCAGTGG
DraII 644 AGGACCT [GA]G^G[ACGT]CC[CT]
2187 AGGACCT
EcoO109I 644 AGGACCT [GA]G^G[ACGT]CC[CT]
2187 AGGACCT
EcoRII 630 CCTGG ^CC[AT]GG
1438 CCAGG
FbaI 744 TGATCA T^GATCA
1932 TGATCA
HaeIII 285 GGCC GG^CC
1329 GGCC
MslI 1089 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1114 CACCCCAGTG
MvaI 630 CCTGG CC^[AT]GG
1438 CCAGG
NspV 650 TTCGAA TT^CGAA
1284 TTCGAA
PpuMI 644 AGGACCT [GA]G^G[AT]CC[CT]
2187 AGGACCT
PstI 727 CTGCAG CTGCA^G
2005 CTGCAG
ScrFI 630 CCTGG CC^[ACGT]GG
1438 CCAGG
SfuI 650 TTCGAA TT^CGAA
1284 TTCGAA
SspBI 278 TGTACA T^GTACA
2356 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BseRI BsiYI BslI
BsmAI BsrI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRI Fnu4HI FokI HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII