##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AocI Aor51HI AscI
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BglI
BlnI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FauI FseI
FspI HaeII Hin1I KasI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1655 GGTACC G^GTACC
AccI 4000 GTCTAC GT^[AC][GT]AC
ApaI 5119 GGGCCC GGGCC^C
Asp718I 1655 GGTACC G^GTACC
BanI 1655 GGTACC G^G[CT][GA]CC
BanII 5119 GGGCCC G[GA]GC[CT]^C
BbsI 4991 GAAGAC GAAGAC(2/6)
BbvCI 3746 CCTCAGC CCTCAGC(-5/-2)
BciVI 2467 GGATAC GTATCC(6/5)
BcnI 231 CCGGG CC^[GC]GG
BpuAI 4991 GAAGAC GAAGAC(2/6)
BsaAI 1968 TACGTG [CT]AC^GT[GA]
BsaBI 3424 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1658 ACCGGA [AT]^CCGG[AT]
BsgI 324 GTGCAG GTGCAG(16/14)
BsmBI 1717 GAGACG CGTCTC(1/5)
BspMI 245 GCAGGT ACCTGC(4/8)
BsrDI 4089 GCAATG GCAATG(2/0)
BssSI 1947 CACGAG CACGAG(-5/-1)
BstAPI 3356 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2038 TTCGAA TT^CGAA
BstXI 637 CCACAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3349 GCGC GCG^C
DrdI 544 GACAGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 875 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 3349 GCGC GCG^C
HinP1I 3349 GCGC G^CGC
HpaI 435 GTTAAC GTT^AAC
KpnI 1655 GGTACC GGTAC^C
MamI 3424 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 4430 CAGCTG C[AC]G^C[GT]G
NciI 231 CCGGG CC^[GC]GG
NdeI 1879 CATATG CA^TATG
NspV 2038 TTCGAA TT^CGAA
PflMI 5238 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 4430 CAGCTG CAG^CTG
SexAI 430 ACCTGGT A^CC[AT]GGT
SfuI 2038 TTCGAA TT^CGAA
SpeI 4152 ACTAGT A^CTAGT
StuI 676 AGGCCT AGG^CCT
Van91I 5238 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1551 CCAAACCAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 1757 CTTAAG C^TTAAG
4274 CTTAAG
Alw44I 312 GTGCAC G^TGCAC
2887 GTGCAC
AlwNI 802 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
5239 CAGCGTCTG
ApaLI 312 GTGCAC G^TGCAC
2887 GTGCAC
BamHI 902 GGATCC G^GATCC
1961 GGATCC
BfrI 1757 CTTAAG C^TTAAG
4274 CTTAAG
BpmI 1588 CTCCAG CTGGAG(16/14)
145 CTGGAG
BsaI 2026 GAGACC GGTCTC(1/5)
1777 GGTCTC
BseRI 613 CTCCTC GAGGAG(10/8)
3176 CTCCTC
BspHI 1088 TCATGA T^CATGA
2537 TCATGA
DsaI 922 CCGTGG C^C[GA][CT]GG
5263 CCGTGG
EcoT22I 5063 ATGCAT ATGCA^T
5349 ATGCAT
NsiI 5063 ATGCAT ATGCA^T
5349 ATGCAT
RcaI 1088 TCATGA T^CATGA
2537 TCATGA
ScaI 4452 AGTACT AGT^ACT
4685 AGTACT
SfcI 1988 CTGTAG C^T[GA][CT]AG
3331 CTGTAG
SphI 3361 GCATGC GCATG^C
5351 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BbvI BclI BfaI
BglII BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI