##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AocI AscI AseI AsnI
AspEI AviII AvrII BbeI BbrPI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco81I EcoRI EcoRV
FspI HindIII HpaI KasI MfeI
MluI MroI MunI NarI NheI
NotI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfoI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 4155 ACGTGT A^C[GA][CT]GT
AgeI 3651 ACCGGT A^CCGGT
Alw44I 3064 GTGCAC G^TGCAC
Aor51HI 236 AGCGCT AGC^GCT
ApaLI 3064 GTGCAC G^TGCAC
AspI 4424 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 259 GGATCC G^GATCC
BcgI 165 GCAGTCCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3712 GGATAC GTATCC(6/5)
BglI 1894 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 852 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3651 ACCGGT [AT]^CCGG[AT]
BsmBI 1440 CGTCTC CGTCTC(1/5)
BspHI 4094 TCATGA T^CATGA
BstEII 2681 GGTCACC G^GT[ACGT]ACC
BstPI 2681 GGTCACC G^GT[ACGT]ACC
BstXI 2806 CCAACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1349 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 394 CGGCCG C^GGCCG
EclXI 394 CGGCCG C^GGCCG
Eco47III 236 AGCGCT AGC^GCT
Eco52I 394 CGGCCG C^GGCCG
EcoO65I 2681 GGTCACC G^GT[ACGT]ACC
EcoT22I 3531 ATGCAT ATGCA^T
FseI 395 GGCCGGCC GGCCGG^CC
MamI 852 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 226 TCGCGA TCG^CGA
NsiI 3531 ATGCAT ATGCA^T
PflFI 4424 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3651 ACCGGT A^CCGGT
RcaI 4094 TCATGA T^CATGA
SbfI 1760 CCTGCAGG CCTGCA^GG
SfiI 1893 GGCCCCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1760 CCTGCAGG CCTGCA^GG
SspI 710 AATATT AAT^ATT
StuI 2161 AGGCCT AGG^CCT
Tth111I 4424 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1318 CCATGGAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3155 GGTACC G^GTACC
3850 GGTACC
AcyI 149 GGCGTC G[GA]^CG[CT]C
1224 GACGCC
Asp700I 587 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2894 GAATCGGTTC
Asp718I 3155 GGTACC G^GTACC
3850 GGTACC
BalI 1315 TGGCCA TGG^CCA
2815 TGGCCA
BbvCI 2364 CCTCAGC CCTCAGC(-5/-2)
2822 CCTCAGC
BclI 1379 TGATCA T^GATCA
1552 TGATCA
BglII 1192 AGATCT A^GATCT
1706 AGATCT
BlpI 2714 GCTCAGC GC^T[ACGT]AGC
4175 GCTCAGC
Bpu1102I 2714 GCTCAGC GC^T[ACGT]AGC
4175 GCTCAGC
BsaAI 1023 TACGTG [CT]AC^GT[GA]
4154 TACGTG
BsaHI 149 GGCGTC G[GA]^CG[CT]C
1224 GACGCC
BsaI 176 GGTCTC GGTCTC(1/5)
222 GGTCTC
BsiEI 394 CGGCCG CG[GA][CT]^CG
467 CGACCG
Bsp1407I 3938 TGTACA T^GTACA
4249 TGTACA
BsrGI 3938 TGTACA T^GTACA
4249 TGTACA
BssSI 3560 CTCGTG CACGAG(-5/-1)
3969 CTCGTG
CelII 2714 GCTCAGC GC^T[ACGT]AGC
4175 GCTCAGC
DraI 827 TTTAAA TTT^AAA
4130 TTTAAA
DraIII 720 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1665 CACACAGTG
FbaI 1379 TGATCA T^GATCA
1552 TGATCA
Hin1I 149 GGCGTC G[GA]^CG[CT]C
1224 GACGCC
HincII 1740 GTCAAC GT[CT]^[GA]AC
2706 GTTGAC
HindII 1740 GTCAAC GT[CT]^[GA]AC
2706 GTTGAC
KpnI 3155 GGTACC GGTAC^C
3850 GGTACC
MluNI 1315 TGGCCA TGG^CCA
2815 TGGCCA
MscI 1315 TGGCCA TGG^CCA
2815 TGGCCA
NaeI 396 GCCGGC GCC^GGC
3521 GCCGGC
NdeI 1149 CATATG CA^TATG
2295 CATATG
NgoMIV 396 GCCGGC G^CCGGC
3521 GCCGGC
SapI 1617 GAAGAGC GCTCTTC(1/4)
199 GCTCTTC
SmaI 2959 CCCGGG CCC^GGG
3889 CCCGGG
SmlI 1833 CTTGAG C^T[CT][GA]AG
3644 CTTGAG
SphI 1459 GCATGC GCATG^C
1940 GCATGC
SspBI 3938 TGTACA T^GTACA
4249 TGTACA
XmaCI 2959 CCCGGG C^CCGGG
3889 CCCGGG
XmaI 2959 CCCGGG C^CCGGG
3889 CCCGGG
XmnI 587 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2894 GAATCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII