##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AviII       BbeI        BlpI        Bpu1102I    
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     FseI        
FspI        HaeII       KasI        KpnI        KspI        
MroI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PinAI       
PmeI        Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        Sse8387I    
SwaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        3090  GACGTC               GACGT^C
AflII        5555  CTTAAG               C^TTAAG
ApaI          900  GGGCCC               GGGCC^C
AvrII        4114  CCTAGG               C^CTAGG
BamHI        2916  GGATCC               G^GATCC
BbrPI         620  CACGTG               CAC^GTG
BbvCI         875  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3962  GGATAC               GTATCC(6/5)
BclI         6097  TGATCA               T^GATCA
BfrI         5555  CTTAAG               C^TTAAG
BlnI         4114  CCTAGG               C^CTAGG
BsaAI         620  CACGTG               [CT]AC^GT[GA]
BsmI         4302  GAATGC               GAATGC(1/-1)
Bsp1407I     3469  TGTACA               T^GTACA
BspLU11I     4981  ACATGT               A^CATGT
BsrGI        3469  TGTACA               T^GTACA
BssSI        4513  CTCGTG               CACGAG(-5/-1)
EcoRI         281  GAATTC               G^AATTC
EcoRV        2989  GATATC               GAT^ATC
FbaI         6097  TGATCA               T^GATCA
HincII       5330  GTTAAC               GT[CT]^[GA]AC
HindII       5330  GTTAAC               GT[CT]^[GA]AC
HpaI         5330  GTTAAC               GTT^AAC
MfeI          435  CAATTG               C^AATTG
MluI         1297  ACGCGT               A^CGCGT
MunI          435  CAATTG               C^AATTG
PaeR7I        228  CTCGAG               C^TCGAG
PmaCI         620  CACGTG               CAC^GTG
PmlI          620  CACGTG               CAC^GTG
PshAI        5160  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         108  CAGCTG               CAG^CTG
SacI         3631  GAGCTC               GAGCT^C
SphI         5634  GCATGC               GCATG^C
SspBI        3469  TGTACA               T^GTACA
XbaI         2272  TCTAGA               T^CTAGA
XhoI          228  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI         2122  GTCTAC               GT^[AC][GT]AC
             5935  GTAGAC              
AccII        1298  CGCG                 CG^CG
             2568  CGCG                
AflIII       1297  ACGCGT               A^C[GA][CT]GT
             4981  ACATGT              
AocI         4037  CCTGAGG              CC^T[ACGT]AGG
             4282  CCTCAGG             
AspI          821  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4214  GACTATGTC           
BglI         1147  GCCCTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4175  GCCCGGCTGGC         
BsaBI        2812  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3992  GATAACTATC          
BsaI         4154  GAGACC               GGTCTC(1/5)
              211  GGTCTC              
BstBI         390  TTCGAA               TT^CGAA
             4878  TTCGAA              
BstUI        1298  CGCG                 CG^CG
             2568  CGCG                
Bsu36I       4037  CCTGAGG              CC^T[ACGT]AGG
             4282  CCTCAGG             
CfoI         2605  GCGC                 GCG^C
             3700  GCGC                
DraIII       4937  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5982  CACACTGTG           
DrdI         2249  GACTTCACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3794  GACATGGATGTC        
Eco81I       4037  CCTGAGG              CC^T[ACGT]AGG
             4282  CCTCAGG             
EcoT22I       547  ATGCAT               ATGCA^T
             4395  ATGCAT              
FauI         3141  CCCGC                CCCGC(4/6)
             2569  GCGGG               
HgaI          625  GACGC                GACGC(5/10)
             3232  GACGC               
HhaI         2605  GCGC                 GCG^C
             3700  GCGC                
HinP1I       2605  GCGC                 G^CGC
             3700  GCGC                
MamI         2812  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3992  GATAACTATC          
MspA1I        108  CAGCTG               C[AC]G^C[GT]G
             4902  CCGCTG              
MvnI         1298  CGCG                 CG^CG
             2568  CGCG                
NsiI          547  ATGCAT               ATGCA^T
             4395  ATGCAT              
NspV          390  TTCGAA               TT^CGAA
             4878  TTCGAA              
PflFI         821  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4214  GACTATGTC           
PpuMI        2423  GGGTCCC              [GA]G^G[AT]CC[CT]
             3638  GGGTCCC             
ScaI         1372  AGTACT               AGT^ACT
             1998  AGTACT              
SfuI          390  TTCGAA               TT^CGAA
             4878  TTCGAA              
SmaI         2541  CCCGGG               CCC^GGG
             4158  CCCGGG              
Tth111I       821  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4214  GACTATGTC           
XmaCI        2541  CCCGGG               C^CCGGG
             4158  CCCGGG              
XmaI         2541  CCCGGG               C^CCGGG
             4158  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       Hin1I       
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PshBI       PstI        
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI