##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AviII BbeI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII KasI KpnI KspI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3090 GACGTC GACGT^C
AflII 5555 CTTAAG C^TTAAG
ApaI 900 GGGCCC GGGCC^C
AvrII 4114 CCTAGG C^CTAGG
BamHI 2916 GGATCC G^GATCC
BbrPI 620 CACGTG CAC^GTG
BbvCI 875 GCTGAGG CCTCAGC(-5/-2)
BciVI 3962 GGATAC GTATCC(6/5)
BclI 6097 TGATCA T^GATCA
BfrI 5555 CTTAAG C^TTAAG
BlnI 4114 CCTAGG C^CTAGG
BsaAI 620 CACGTG [CT]AC^GT[GA]
BsmI 4302 GAATGC GAATGC(1/-1)
Bsp1407I 3469 TGTACA T^GTACA
BspLU11I 4981 ACATGT A^CATGT
BsrGI 3469 TGTACA T^GTACA
BssSI 4513 CTCGTG CACGAG(-5/-1)
EcoRI 281 GAATTC G^AATTC
EcoRV 2989 GATATC GAT^ATC
FbaI 6097 TGATCA T^GATCA
HincII 5330 GTTAAC GT[CT]^[GA]AC
HindII 5330 GTTAAC GT[CT]^[GA]AC
HpaI 5330 GTTAAC GTT^AAC
MfeI 435 CAATTG C^AATTG
MluI 1297 ACGCGT A^CGCGT
MunI 435 CAATTG C^AATTG
PaeR7I 228 CTCGAG C^TCGAG
PmaCI 620 CACGTG CAC^GTG
PmlI 620 CACGTG CAC^GTG
PshAI 5160 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 108 CAGCTG CAG^CTG
SacI 3631 GAGCTC GAGCT^C
SphI 5634 GCATGC GCATG^C
SspBI 3469 TGTACA T^GTACA
XbaI 2272 TCTAGA T^CTAGA
XhoI 228 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2122 GTCTAC GT^[AC][GT]AC
5935 GTAGAC
AccII 1298 CGCG CG^CG
2568 CGCG
AflIII 1297 ACGCGT A^C[GA][CT]GT
4981 ACATGT
AocI 4037 CCTGAGG CC^T[ACGT]AGG
4282 CCTCAGG
AspI 821 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4214 GACTATGTC
BglI 1147 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4175 GCCCGGCTGGC
BsaBI 2812 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3992 GATAACTATC
BsaI 4154 GAGACC GGTCTC(1/5)
211 GGTCTC
BstBI 390 TTCGAA TT^CGAA
4878 TTCGAA
BstUI 1298 CGCG CG^CG
2568 CGCG
Bsu36I 4037 CCTGAGG CC^T[ACGT]AGG
4282 CCTCAGG
CfoI 2605 GCGC GCG^C
3700 GCGC
DraIII 4937 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
5982 CACACTGTG
DrdI 2249 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3794 GACATGGATGTC
Eco81I 4037 CCTGAGG CC^T[ACGT]AGG
4282 CCTCAGG
EcoT22I 547 ATGCAT ATGCA^T
4395 ATGCAT
FauI 3141 CCCGC CCCGC(4/6)
2569 GCGGG
HgaI 625 GACGC GACGC(5/10)
3232 GACGC
HhaI 2605 GCGC GCG^C
3700 GCGC
HinP1I 2605 GCGC G^CGC
3700 GCGC
MamI 2812 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3992 GATAACTATC
MspA1I 108 CAGCTG C[AC]G^C[GT]G
4902 CCGCTG
MvnI 1298 CGCG CG^CG
2568 CGCG
NsiI 547 ATGCAT ATGCA^T
4395 ATGCAT
NspV 390 TTCGAA TT^CGAA
4878 TTCGAA
PflFI 821 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4214 GACTATGTC
PpuMI 2423 GGGTCCC [GA]G^G[AT]CC[CT]
3638 GGGTCCC
ScaI 1372 AGTACT AGT^ACT
1998 AGTACT
SfuI 390 TTCGAA TT^CGAA
4878 TTCGAA
SmaI 2541 CCCGGG CCC^GGG
4158 CCCGGG
Tth111I 821 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4214 GACTATGTC
XmaCI 2541 CCCGGG C^CCGGG
4158 CCCGGG
XmaI 2541 CCCGGG C^CCGGG
4158 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII Hin1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI