################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaII AviII AvrII BalI BamHI BbeI BbvCI BcgI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MflI MluI MluNI MroI MscI MspA1I NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2037 GTAGAC GT^[AC][GT]AC AccII 2709 CGCG CG^CG AlwI 2675 GGATC GGATC(4/5) AlwNI 2414 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 2219 GAGCTC G[AT]GC[AT]^C AspI 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 553 CCCGAG C^[CT]CG[GA]G BanI 32 GGTGCC G^G[CT][GA]CC BanII 2219 GAGCTC G[GA]GC[CT]^C BbrPI 2270 CACGTG CAC^GTG BpmI 1979 CTGGAG CTGGAG(16/14) BsaBI 2060 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2088 GAGACC GGTCTC(1/5) BsaJI 2107 CCAGGG C^C[ACGT][ACGT]GG BsiHKAI 2219 GAGCTC G[AT]GC[AT]^C BsmBI 194 GAGACG CGTCTC(1/5) BsoBI 553 CCCGAG C^[CT]CG[GA]G BspHI 2062 TCATGA T^CATGA BspLU11I 325 ACATGT A^CATGT BstUI 2709 CGCG CG^CG Cfr13I 916 GGCCC G^G[ACGT]CC DraI 932 TTTAAA TTT^AAA EcoNI 1870 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2149 GAATTC G^AATTC EcoT22I 2356 ATGCAT ATGCA^T HgaI 2710 GCGTC GACGC(5/10) HpaI 2825 GTTAAC GTT^AAC MamI 2060 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2709 CGCG CG^CG NlaIV 32 GGTGCC GG[ACGT]^[ACGT]CC NsiI 2356 ATGCAT ATGCA^T NspI 325 ACATGT [GA]CATG^[CT] PflFI 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1810 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2270 CACGTG CAC^GTG PmlI 2270 CACGTG CAC^GTG RcaI 2062 TCATGA T^CATGA SacI 2219 GAGCTC GAGCT^C SapI 1610 GAAGAGC GCTCTTC(1/4) Sau96I 916 GGCCC G^G[ACGT]CC Tth111I 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1810 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1776 CCAGGCTCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 1753 CCGC CCGC(-3/-1) 978 GCGG AflIII 325 ACATGT A^C[GA][CT]GT 2269 ACACGT AseI 1363 ATTAAT AT^TAAT 2893 ATTAAT AsnI 1363 ATTAAT AT^TAAT 2893 ATTAAT Asp700I 1695 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1865 GAAGACCTTC BglI 713 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 815 GCCTGGAAGGC BmyI 2110 GGGCAC G[AGT]GC[ACT]^C 2219 GAGCTC BsaAI 885 TACGTG [CT]AC^GT[GA] 2270 CACGTG BseRI 380 CTCCTC GAGGAG(10/8) 900 CTCCTC BsmFI 890 GGGAC GGGAC(10/14) 551 GTCCC BsmI 2044 GAATGC GAATGC(1/-1) 2591 GAATGC Bsp1286I 2110 GGGCAC G[AGT]GC[ACT]^C 2219 GAGCTC Bsp1407I 1081 TGTACA T^GTACA 2325 TGTACA BspMI 344 ACCTGC ACCTGC(4/8) 1737 ACCTGC BsrFI 811 ACCGGC [GA]^CCGG[CT] 1654 ACCGGC BsrGI 1081 TGTACA T^GTACA 2325 TGTACA Cfr10I 811 ACCGGC [GA]^CCGG[CT] 1654 ACCGGC HaeIII 814 GGCC GG^CC 916 GGCC HapII 812 CCGG C^CGG 1655 CCGG HincII 2382 GTCAAC GT[CT]^[GA]AC 2825 GTTAAC HindII 2382 GTCAAC GT[CT]^[GA]AC 2825 GTTAAC HpaII 812 CCGG C^CGG 1655 CCGG MfeI 365 CAATTG C^AATTG 1032 CAATTG MspI 812 CCGG C^CGG 1655 CCGG MunI 365 CAATTG C^AATTG 1032 CAATTG NdeI 462 CATATG CA^TATG 658 CATATG PshBI 1363 ATTAAT AT^TAAT 2893 ATTAAT SfcI 1516 CTGTAG C^T[GA][CT]AG 2816 CTGTAG SspBI 1081 TGTACA T^GTACA 2325 TGTACA XmnI 1695 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1865 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI ApoI BbsI BbvI BciVI BclI BfaI BpuAI BsgI BsiYI BslI BsmAI BsrI BssKI BstBI BstF5I BstNI Cac8I DdeI DpnII EarI EcoRII FbaI Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NspV PleI RsaI Sau3AI ScrFI SfaNI SfuI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I