##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbvCI       BcgI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       EcoT14I     FauI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HindIII     KasI        KpnI        KspI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         2037  GTAGAC               GT^[AC][GT]AC
AccII        2709  CGCG                 CG^CG
AlwI         2675  GGATC                GGATC(4/5)
AlwNI        2414  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI        2219  GAGCTC               G[AT]GC[AT]^C
AspI          892  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          553  CCCGAG               C^[CT]CG[GA]G
BanI           32  GGTGCC               G^G[CT][GA]CC
BanII        2219  GAGCTC               G[GA]GC[CT]^C
BbrPI        2270  CACGTG               CAC^GTG
BpmI         1979  CTGGAG               CTGGAG(16/14)
BsaBI        2060  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2088  GAGACC               GGTCTC(1/5)
BsaJI        2107  CCAGGG               C^C[ACGT][ACGT]GG
BsiHKAI      2219  GAGCTC               G[AT]GC[AT]^C
BsmBI         194  GAGACG               CGTCTC(1/5)
BsoBI         553  CCCGAG               C^[CT]CG[GA]G
BspHI        2062  TCATGA               T^CATGA
BspLU11I      325  ACATGT               A^CATGT
BstUI        2709  CGCG                 CG^CG
Cfr13I        916  GGCCC                G^G[ACGT]CC
DraI          932  TTTAAA               TTT^AAA
EcoNI        1870  CCTTCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2149  GAATTC               G^AATTC
EcoT22I      2356  ATGCAT               ATGCA^T
HgaI         2710  GCGTC                GACGC(5/10)
HpaI         2825  GTTAAC               GTT^AAC
MamI         2060  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2709  CGCG                 CG^CG
NlaIV          32  GGTGCC               GG[ACGT]^[ACGT]CC
NsiI         2356  ATGCAT               ATGCA^T
NspI          325  ACATGT               [GA]CATG^[CT]
PflFI         892  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1810  CCAAAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2270  CACGTG               CAC^GTG
PmlI         2270  CACGTG               CAC^GTG
RcaI         2062  TCATGA               T^CATGA
SacI         2219  GAGCTC               GAGCT^C
SapI         1610  GAAGAGC              GCTCTTC(1/4)
Sau96I        916  GGCCC                G^G[ACGT]CC
Tth111I       892  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1810  CCAAAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1776  CCAGGCTCAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI         1753  CCGC                 CCGC(-3/-1)
              978  GCGG                
AflIII        325  ACATGT               A^C[GA][CT]GT
             2269  ACACGT              
AseI         1363  ATTAAT               AT^TAAT
             2893  ATTAAT              
AsnI         1363  ATTAAT               AT^TAAT
             2893  ATTAAT              
Asp700I      1695  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1865  GAAGACCTTC          
BglI          713  GCCTTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              815  GCCTGGAAGGC         
BmyI         2110  GGGCAC               G[AGT]GC[ACT]^C
             2219  GAGCTC              
BsaAI         885  TACGTG               [CT]AC^GT[GA]
             2270  CACGTG              
BseRI         380  CTCCTC               GAGGAG(10/8)
              900  CTCCTC              
BsmFI         890  GGGAC                GGGAC(10/14)
              551  GTCCC               
BsmI         2044  GAATGC               GAATGC(1/-1)
             2591  GAATGC              
Bsp1286I     2110  GGGCAC               G[AGT]GC[ACT]^C
             2219  GAGCTC              
Bsp1407I     1081  TGTACA               T^GTACA
             2325  TGTACA              
BspMI         344  ACCTGC               ACCTGC(4/8)
             1737  ACCTGC              
BsrFI         811  ACCGGC               [GA]^CCGG[CT]
             1654  ACCGGC              
BsrGI        1081  TGTACA               T^GTACA
             2325  TGTACA              
Cfr10I        811  ACCGGC               [GA]^CCGG[CT]
             1654  ACCGGC              
HaeIII        814  GGCC                 GG^CC
              916  GGCC                
HapII         812  CCGG                 C^CGG
             1655  CCGG                
HincII       2382  GTCAAC               GT[CT]^[GA]AC
             2825  GTTAAC              
HindII       2382  GTCAAC               GT[CT]^[GA]AC
             2825  GTTAAC              
HpaII         812  CCGG                 C^CGG
             1655  CCGG                
MfeI          365  CAATTG               C^AATTG
             1032  CAATTG              
MspI          812  CCGG                 C^CGG
             1655  CCGG                
MunI          365  CAATTG               C^AATTG
             1032  CAATTG              
NdeI          462  CATATG               CA^TATG
              658  CATATG              
PshBI        1363  ATTAAT               AT^TAAT
             2893  ATTAAT              
SfcI         1516  CTGTAG               C^T[GA][CT]AG
             2816  CTGTAG              
SspBI        1081  TGTACA               T^GTACA
             2325  TGTACA              
XmnI         1695  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1865  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbsI        
BbvI        BciVI       BclI        BfaI        BpuAI       
BsgI        BsiYI       BslI        BsmAI       BsrI        
BssKI       BstBI       BstF5I      BstNI       Cac8I       
DdeI        DpnII       EarI        EcoRII      FbaI        
Fnu4HI      FokI        HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NspV        PleI        
RsaI        Sau3AI      ScrFI       SfaNI       SfuI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I