##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaII AviII AvrII BalI
BamHI BbeI BbvCI BcgI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT14I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII KasI KpnI KspI MflI
MluI MluNI MroI MscI MspA1I
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2037 GTAGAC GT^[AC][GT]AC
AccII 2709 CGCG CG^CG
AlwI 2675 GGATC GGATC(4/5)
AlwNI 2414 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 2219 GAGCTC G[AT]GC[AT]^C
AspI 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 553 CCCGAG C^[CT]CG[GA]G
BanI 32 GGTGCC G^G[CT][GA]CC
BanII 2219 GAGCTC G[GA]GC[CT]^C
BbrPI 2270 CACGTG CAC^GTG
BpmI 1979 CTGGAG CTGGAG(16/14)
BsaBI 2060 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2088 GAGACC GGTCTC(1/5)
BsaJI 2107 CCAGGG C^C[ACGT][ACGT]GG
BsiHKAI 2219 GAGCTC G[AT]GC[AT]^C
BsmBI 194 GAGACG CGTCTC(1/5)
BsoBI 553 CCCGAG C^[CT]CG[GA]G
BspHI 2062 TCATGA T^CATGA
BspLU11I 325 ACATGT A^CATGT
BstUI 2709 CGCG CG^CG
Cfr13I 916 GGCCC G^G[ACGT]CC
DraI 932 TTTAAA TTT^AAA
EcoNI 1870 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2149 GAATTC G^AATTC
EcoT22I 2356 ATGCAT ATGCA^T
HgaI 2710 GCGTC GACGC(5/10)
HpaI 2825 GTTAAC GTT^AAC
MamI 2060 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2709 CGCG CG^CG
NlaIV 32 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 2356 ATGCAT ATGCA^T
NspI 325 ACATGT [GA]CATG^[CT]
PflFI 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1810 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2270 CACGTG CAC^GTG
PmlI 2270 CACGTG CAC^GTG
RcaI 2062 TCATGA T^CATGA
SacI 2219 GAGCTC GAGCT^C
SapI 1610 GAAGAGC GCTCTTC(1/4)
Sau96I 916 GGCCC G^G[ACGT]CC
Tth111I 892 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1810 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1776 CCAGGCTCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 1753 CCGC CCGC(-3/-1)
978 GCGG
AflIII 325 ACATGT A^C[GA][CT]GT
2269 ACACGT
AseI 1363 ATTAAT AT^TAAT
2893 ATTAAT
AsnI 1363 ATTAAT AT^TAAT
2893 ATTAAT
Asp700I 1695 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1865 GAAGACCTTC
BglI 713 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
815 GCCTGGAAGGC
BmyI 2110 GGGCAC G[AGT]GC[ACT]^C
2219 GAGCTC
BsaAI 885 TACGTG [CT]AC^GT[GA]
2270 CACGTG
BseRI 380 CTCCTC GAGGAG(10/8)
900 CTCCTC
BsmFI 890 GGGAC GGGAC(10/14)
551 GTCCC
BsmI 2044 GAATGC GAATGC(1/-1)
2591 GAATGC
Bsp1286I 2110 GGGCAC G[AGT]GC[ACT]^C
2219 GAGCTC
Bsp1407I 1081 TGTACA T^GTACA
2325 TGTACA
BspMI 344 ACCTGC ACCTGC(4/8)
1737 ACCTGC
BsrFI 811 ACCGGC [GA]^CCGG[CT]
1654 ACCGGC
BsrGI 1081 TGTACA T^GTACA
2325 TGTACA
Cfr10I 811 ACCGGC [GA]^CCGG[CT]
1654 ACCGGC
HaeIII 814 GGCC GG^CC
916 GGCC
HapII 812 CCGG C^CGG
1655 CCGG
HincII 2382 GTCAAC GT[CT]^[GA]AC
2825 GTTAAC
HindII 2382 GTCAAC GT[CT]^[GA]AC
2825 GTTAAC
HpaII 812 CCGG C^CGG
1655 CCGG
MfeI 365 CAATTG C^AATTG
1032 CAATTG
MspI 812 CCGG C^CGG
1655 CCGG
MunI 365 CAATTG C^AATTG
1032 CAATTG
NdeI 462 CATATG CA^TATG
658 CATATG
PshBI 1363 ATTAAT AT^TAAT
2893 ATTAAT
SfcI 1516 CTGTAG C^T[GA][CT]AG
2816 CTGTAG
SspBI 1081 TGTACA T^GTACA
2325 TGTACA
XmnI 1695 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1865 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BciVI BclI BfaI BpuAI
BsgI BsiYI BslI BsmAI BsrI
BssKI BstBI BstF5I BstNI Cac8I
DdeI DpnII EarI EcoRII FbaI
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NspV PleI
RsaI Sau3AI ScrFI SfaNI SfuI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I