##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BbeI        BfrI        BlnI        BsaI        BsiEI       
BsiWI       BspHI       BspLU11I    BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      CpoI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FseI        FspI        HaeII       HincII      
HindII      HpaI        KasI        KpnI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        TfiI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII        1934  CGCG                 CG^CG
AccIII       2197  TCCGGA               T^CCGGA
AhdI            6  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2030  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI           6  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1045  GGATCC               G^GATCC
BcgI         3166  CGAGGGGCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3277  GTATCC               GTATCC(6/5)
BclI         1226  TGATCA               T^GATCA
BglII        1711  AGATCT               A^GATCT
BsaBI        3515  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2197  TCCGGA               [AT]^CCGG[AT]
BseAI        2197  TCCGGA               T^CCGGA
BsmI          351  GAATGC               GAATGC(1/-1)
Bsp1407I      175  TGTACA               T^GTACA
BspDI         687  ATCGAT               AT^CGAT
BspEI        2197  TCCGGA               T^CCGGA
BsrGI         175  TGTACA               T^GTACA
BssSI        1634  CTCGTG               CACGAG(-5/-1)
BstUI        1934  CGCG                 CG^CG
ClaI          687  ATCGAT               AT^CGAT
Eam1105I        6  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         1226  TGATCA               T^GATCA
KspI         1933  CCGCGG               CCGC^GG
MamI         3515  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2197  TCCGGA               T^CCGGA
MvnI         1934  CGCG                 CG^CG
SacII        1933  CCGCGG               CCGC^GG
SapI         2279  GAAGAGC              GCTCTTC(1/4)
SbfI         1691  CCTGCAGG             CCTGCA^GG
SmaI          885  CCCGGG               CCC^GGG
SphI         2395  GCATGC               GCATG^C
Sse8387I     1691  CCTGCAGG             CCTGCA^GG
SspBI         175  TGTACA               T^GTACA
SspI         3788  AATATT               AAT^ATT
XmaCI         885  CCCGGG               C^CCGGG
XmaI          885  CCCGGG               C^CCGGG
XmnI         2030  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII        2319  GACGTC               GACGT^C
             3360  GACGTC              
AccI          521  GTCTAC               GT^[AC][GT]AC
             1548  GTCTAC              
AcsI         2088  AAATTT               [GA]^AATT[CT]
             2460  AAATTC              
AcyI         2319  GACGTC               G[GA]^CG[CT]C
             3360  GACGTC              
ApaI         3452  GGGCCC               GGGCC^C
             3491  GGGCCC              
ApoI         2088  AAATTT               [GA]^AATT[CT]
             2460  AAATTC              
AvaI          885  CCCGGG               C^[CT]CG[GA]G
             3164  CCCGAG              
BanI         2684  GGTGCC               G^G[CT][GA]CC
             3369  GGCACC              
BsaHI        2319  GACGTC               G[GA]^CG[CT]C
             3360  GACGTC              
BsoBI         885  CCCGGG               C^[CT]CG[GA]G
             3164  CCCGAG              
BspMI        1947  ACCTGC               ACCTGC(4/8)
             2350  ACCTGC              
BsrBI         741  CCGCTC               CCGCTC(-3/-3)
             3254  GAGCGG              
DrdI         2130  GACATGACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3537  GACGGCATGGTC        
EcoT22I       450  ATGCAT               ATGCA^T
              973  ATGCAT              
HgaI         1430  GACGC                GACGC(5/10)
             3228  GCGTC               
Hin1I        2319  GACGTC               G[GA]^CG[CT]C
             3360  GACGTC              
HindIII      1781  AAGCTT               A^AGCTT
             2243  AAGCTT              
NsiI          450  ATGCAT               ATGCA^T
              973  ATGCAT              
SexAI        1465  ACCTGGT              A^CC[AT]GGT
             2191  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AspI        AvaII       BalI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII