################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BbeI BfrI BlnI BsaI BsiEI BsiWI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HincII HindII HpaI KasI KpnI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 1934 CGCG CG^CG AccIII 2197 TCCGGA T^CCGGA AhdI 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2030 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1045 GGATCC G^GATCC BcgI 3166 CGAGGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3277 GTATCC GTATCC(6/5) BclI 1226 TGATCA T^GATCA BglII 1711 AGATCT A^GATCT BsaBI 3515 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2197 TCCGGA [AT]^CCGG[AT] BseAI 2197 TCCGGA T^CCGGA BsmI 351 GAATGC GAATGC(1/-1) Bsp1407I 175 TGTACA T^GTACA BspDI 687 ATCGAT AT^CGAT BspEI 2197 TCCGGA T^CCGGA BsrGI 175 TGTACA T^GTACA BssSI 1634 CTCGTG CACGAG(-5/-1) BstUI 1934 CGCG CG^CG ClaI 687 ATCGAT AT^CGAT Eam1105I 6 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1226 TGATCA T^GATCA KspI 1933 CCGCGG CCGC^GG MamI 3515 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2197 TCCGGA T^CCGGA MvnI 1934 CGCG CG^CG SacII 1933 CCGCGG CCGC^GG SapI 2279 GAAGAGC GCTCTTC(1/4) SbfI 1691 CCTGCAGG CCTGCA^GG SmaI 885 CCCGGG CCC^GGG SphI 2395 GCATGC GCATG^C Sse8387I 1691 CCTGCAGG CCTGCA^GG SspBI 175 TGTACA T^GTACA SspI 3788 AATATT AAT^ATT XmaCI 885 CCCGGG C^CCGGG XmaI 885 CCCGGG C^CCGGG XmnI 2030 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AatII 2319 GACGTC GACGT^C 3360 GACGTC AccI 521 GTCTAC GT^[AC][GT]AC 1548 GTCTAC AcsI 2088 AAATTT [GA]^AATT[CT] 2460 AAATTC AcyI 2319 GACGTC G[GA]^CG[CT]C 3360 GACGTC ApaI 3452 GGGCCC GGGCC^C 3491 GGGCCC ApoI 2088 AAATTT [GA]^AATT[CT] 2460 AAATTC AvaI 885 CCCGGG C^[CT]CG[GA]G 3164 CCCGAG BanI 2684 GGTGCC G^G[CT][GA]CC 3369 GGCACC BsaHI 2319 GACGTC G[GA]^CG[CT]C 3360 GACGTC BsoBI 885 CCCGGG C^[CT]CG[GA]G 3164 CCCGAG BspMI 1947 ACCTGC ACCTGC(4/8) 2350 ACCTGC BsrBI 741 CCGCTC CCGCTC(-3/-3) 3254 GAGCGG DrdI 2130 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3537 GACGGCATGGTC EcoT22I 450 ATGCAT ATGCA^T 973 ATGCAT HgaI 1430 GACGC GACGC(5/10) 3228 GCGTC Hin1I 2319 GACGTC G[GA]^CG[CT]C 3360 GACGTC HindIII 1781 AAGCTT A^AGCTT 2243 AAGCTT NsiI 450 ATGCAT ATGCA^T 973 ATGCAT SexAI 1465 ACCTGGT A^CC[AT]GGT 2191 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AspI AvaII BalI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII