##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BcnI BglI BlnI BpmI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstUI BstXI Bsu36I
CfoI Cfr10I CpoI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3628 GTATAC GT^[AC][GT]AC
AclI 919 AACGTT AA^CGTT
AspHI 1039 GAGCAC G[AT]GC[AT]^C
BanII 470 GGGCTC G[GA]GC[CT]^C
BclI 545 TGATCA T^GATCA
BglII 86 AGATCT A^GATCT
BsaI 1337 GAGACC GGTCTC(1/5)
BsaJI 349 CCCAGG C^C[ACGT][ACGT]GG
BsiHKAI 1039 GAGCAC G[AT]GC[AT]^C
BspDI 2671 ATCGAT AT^CGAT
Bst1107I 3628 GTATAC GTA^TAC
BstEII 1523 GGTTACC G^GT[ACGT]ACC
BstPI 1523 GGTTACC G^GT[ACGT]ACC
BstZ17I 3628 GTATAC GTA^TAC
ClaI 2671 ATCGAT AT^CGAT
DraII 559 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 2163 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 559 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1523 GGTTACC G^GT[ACGT]ACC
EcoRI 2421 GAATTC G^AATTC
FauI 3938 CCCGC CCCGC(4/6)
FbaI 545 TGATCA T^GATCA
HindIII 3027 AAGCTT A^AGCTT
HpaI 3132 GTTAAC GTT^AAC
NheI 1986 GCTAGC G^CTAGC
PpuMI 559 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 919 AACGTT AA^CGTT
SwaI 4087 ATTTAAAT ATTT^AAAT
XcmI 438 CCATCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 362 CCGC CCGC(-3/-1)
3939 CCGC
AflIII 901 ACATGT A^C[GA][CT]GT
3624 ACATGT
Asp700I 642 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4243 GAATATTTTC
BciVI 1241 GGATAC GTATCC(6/5)
3418 GTATCC
BlpI 936 GCTCAGC GC^T[ACGT]AGC
3708 GCTCAGC
BmyI 470 GGGCTC G[AGT]GC[ACT]^C
1039 GAGCAC
Bpu1102I 936 GCTCAGC GC^T[ACGT]AGC
3708 GCTCAGC
BseRI 261 GAGGAG GAGGAG(10/8)
2607 GAGGAG
BsiYI 1203 CCAACAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4397 CCACATCTTGG
BslI 1203 CCAACAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4397 CCACATCTTGG
Bsp1286I 470 GGGCTC G[AGT]GC[ACT]^C
1039 GAGCAC
Bsp1407I 2212 TGTACA T^GTACA
3230 TGTACA
BspLU11I 901 ACATGT A^CATGT
3624 ACATGT
BspMI 1340 ACCTGC ACCTGC(4/8)
3544 GCAGGT
BsrGI 2212 TGTACA T^GTACA
3230 TGTACA
CelII 936 GCTCAGC GC^T[ACGT]AGC
3708 GCTCAGC
HaeIII 821 GGCC GG^CC
3107 GGCC
HincII 2053 GTTGAC GT[CT]^[GA]AC
3132 GTTAAC
HindII 2053 GTTGAC GT[CT]^[GA]AC
3132 GTTAAC
MaeII 368 ACGT A^CGT
920 ACGT
MwoI 1102 GCACTGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2443 GCTTAGATTGC
PflMI 1203 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4397 CCACATCTTGG
ScaI 147 AGTACT AGT^ACT
568 AGTACT
SexAI 1145 ACCAGGT A^CC[AT]GGT
2025 ACCAGGT
SspBI 2212 TGTACA T^GTACA
3230 TGTACA
StuI 820 AGGCCT AGG^CCT
3106 AGGCCT
Van91I 1203 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4397 CCACATCTTGG
XbaI 612 TCTAGA T^CTAGA
1007 TCTAGA
XmnI 642 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4243 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BbsI BbvI BfaI BfrI BpuAI
BsmAI BsmFI BsmI BspHI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT22I Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII