##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BamHI BanI BanII
BbeI BcgI BglI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I FauI FseI FspI
Hin1I KasI KpnI KspI MluI
MluNI MroI MscI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 3987 CGCG CG^CG
AciI 822 GCGG CCGC(-3/-1)
AflII 1289 CTTAAG C^TTAAG
AlwI 3877 GATCC GGATC(4/5)
Aor51HI 2288 AGCGCT AGC^GCT
AspHI 112 GTGCTC G[AT]GC[AT]^C
AspI 4298 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2491 CCCGGG C^[CT]CG[GA]G
BbrPI 3881 CACGTG CAC^GTG
BbvCI 4466 GCTGAGG CCTCAGC(-5/-2)
BfrI 1289 CTTAAG C^TTAAG
BglII 4053 AGATCT A^GATCT
BlpI 1059 GCTCAGC GC^T[ACGT]AGC
BmyI 112 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 1059 GCTCAGC GC^T[ACGT]AGC
BsaAI 3881 CACGTG [CT]AC^GT[GA]
BsaBI 3453 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2318 GAGACC GGTCTC(1/5)
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
BsmBI 1039 CGTCTC CGTCTC(1/5)
BsoBI 2491 CCCGGG C^[CT]CG[GA]G
Bsp1286I 112 GTGCTC G[AGT]GC[ACT]^C
BspMI 3182 GCAGGT ACCTGC(4/8)
Bst1107I 2454 GTATAC GTA^TAC
BstUI 3987 CGCG CG^CG
BstYI 4053 AGATCT [GA]^GATC[CT]
BstZ17I 2454 GTATAC GTA^TAC
CelII 1059 GCTCAGC GC^T[ACGT]AGC
DraII 871 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1812 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4417 CCATGG C^C[GA][CT]GG
Eco47III 2288 AGCGCT AGC^GCT
EcoNI 2410 CCTAAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 871 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3835 ATGCAT ATGCA^T
HaeII 2288 AGCGCT [GA]GCGC^[CT]
HapII 2492 CCGG C^CGG
HgaI 2071 GCGTC GACGC(5/10)
HindIII 1273 AAGCTT A^AGCTT
HpaI 3521 GTTAAC GTT^AAC
HpaII 2492 CCGG C^CGG
MamI 3453 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4053 AGATCT [GA]^GATC[CT]
MspI 2492 CCGG C^CGG
MvnI 3987 CGCG CG^CG
NcoI 4417 CCATGG C^CATGG
NdeI 2087 CATATG CA^TATG
NheI 1664 GCTAGC G^CTAGC
NsiI 3835 ATGCAT ATGCA^T
PflFI 4298 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3881 CACGTG CAC^GTG
PmlI 3881 CACGTG CAC^GTG
PpuMI 871 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 3803 ACCTGGT A^CC[AT]GGT
SmaI 2491 CCCGGG CCC^GGG
SpeI 387 ACTAGT A^CTAGT
Tth111I 4298 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 544 CCATCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 4053 AGATCT [GA]^GATC[CT]
XmaCI 2491 CCCGGG C^CCGGG
XmaI 2491 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 3048 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4154 GAATTCCTTC
BclI 614 TGATCA T^GATCA
2144 TGATCA
BcnI 2491 CCCGG CC^[GC]GG
2492 CCGGG
BpmI 2186 CTCCAG CTGGAG(16/14)
3012 CTCCAG
BsmI 1609 GAATGC GAATGC(1/-1)
419 GCATTC
BstAPI 3837 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3954 GCAGATCATGC
BstXI 276 CCAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
400 CCAGTGGAATGG
CfoI 2289 GCGC GCG^C
3996 GCGC
EcoRI 3863 GAATTC G^AATTC
4154 GAATTC
EcoT14I 2991 CCTTGG C^C[AT][AT]GG
4417 CCATGG
FbaI 614 TGATCA T^GATCA
2144 TGATCA
HhaI 2289 GCGC GCG^C
3996 GCGC
HinP1I 2289 GCGC G^CGC
3996 GCGC
HincII 330 GTCAAC GT[CT]^[GA]AC
3521 GTTAAC
HindII 330 GTCAAC GT[CT]^[GA]AC
3521 GTTAAC
MfeI 242 CAATTG C^AATTG
891 CAATTG
MslI 420 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3912 CACTTCAGTG
MunI 242 CAATTG C^AATTG
891 CAATTG
NciI 2491 CCCGG CC^[GC]GG
2492 CCGGG
PflMI 2664 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3798 CCATCACCTGG
PshAI 1280 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2204 GACCAGTGTC
PvuII 964 CAGCTG CAG^CTG
3465 CAGCTG
SapI 114 GCTCTTC GCTCTTC(1/4)
217 GCTCTTC
StuI 1744 AGGCCT AGG^CCT
1769 AGGCCT
StyI 2991 CCTTGG C^C[AT][AT]GG
4417 CCATGG
TaqI 248 TCGA T^CGA
2277 TCGA
TthHB8I 248 TCGA T^CGA
2277 TCGA
Van91I 2664 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3798 CCATCACCTGG
XbaI 1102 TCTAGA T^CTAGA
3428 TCTAGA
XmnI 3048 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4154 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwNI ApoI AseI AsnI AvaII
BbsI BbvI BciVI BfaI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BspHI BspLU11I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoRV Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI