##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BciVI BfrI BlnI
BsiWI BsmBI BsmI BspDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI FspI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1007 CGCG CG^CG
AccIII 1487 TCCGGA T^CCGGA
AcyI 1202 GACGCC G[GA]^CG[CT]C
AocI 3335 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1379 AGCGCT AGC^GCT
AseI 1874 ATTAAT AT^TAAT
AsnI 1874 ATTAAT AT^TAAT
BanI 102 GGCACC G^G[CT][GA]CC
BbrPI 3386 CACGTG CAC^GTG
BcgI 1266 GCAAAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2999 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2999 GCTCAGC GC^T[ACGT]AGC
BsaHI 1202 GACGCC G[GA]^CG[CT]C
BseAI 1487 TCCGGA T^CCGGA
BsiEI 895 CGGTCG CG[GA][CT]^CG
Bsp1407I 4360 TGTACA T^GTACA
BspEI 1487 TCCGGA T^CCGGA
BspHI 1248 TCATGA T^CATGA
BspLU11I 3867 ACATGT A^CATGT
BsrBI 884 GAGCGG CCGCTC(-3/-3)
BsrFI 1352 GCCGGT [GA]^CCGG[CT]
BsrGI 4360 TGTACA T^GTACA
BssSI 2286 CACGAG CACGAG(-5/-1)
BstAPI 446 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1007 CGCG CG^CG
Bsu36I 3335 CCTGAGG CC^T[ACGT]AGG
CelII 2999 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1352 GCCGGT [GA]^CCGG[CT]
EaeI 955 TGGCCG [CT]^GGCC[GA]
Eco47III 1379 AGCGCT AGC^GCT
Eco81I 3335 CCTGAGG CC^T[ACGT]AGG
EcoRI 2645 GAATTC G^AATTC
FauI 725 GCGGG CCCGC(4/6)
HgaI 1202 GACGC GACGC(5/10)
Hin1I 1202 GACGCC G[GA]^CG[CT]C
HindIII 3295 AAGCTT A^AGCTT
HpaI 2270 GTTAAC GTT^AAC
MroI 1487 TCCGGA T^CCGGA
MvnI 1007 CGCG CG^CG
PmaCI 3386 CACGTG CAC^GTG
PmlI 3386 CACGTG CAC^GTG
PshBI 1874 ATTAAT AT^TAAT
RcaI 1248 TCATGA T^CATGA
SacI 2354 GAGCTC GAGCT^C
SbfI 2380 CCTGCAGG CCTGCA^GG
ScaI 2636 AGTACT AGT^ACT
SexAI 2521 ACCTGGT A^CC[AT]GGT
SpeI 2976 ACTAGT A^CTAGT
Sse8387I 2380 CCTGCAGG CCTGCA^GG
SspBI 4360 TGTACA T^GTACA
StuI 1020 AGGCCT AGG^CCT
TaqI 1418 TCGA T^CGA
TthHB8I 1418 TCGA T^CGA
XbaI 3895 TCTAGA T^CTAGA
XcmI 531 CCAGCAAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 329 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
421 CAGAAGCTG
ApaI 385 GGGCCC GGGCC^C
3308 GGGCCC
Asp700I 623 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3532 GAATAATTTC
BbvCI 520 GCTGAGG CCTCAGC(-5/-2)
649 GCTGAGG
BglII 1086 AGATCT A^GATCT
1341 AGATCT
BsaAI 1178 TACGTG [CT]AC^GT[GA]
3386 CACGTG
BsaWI 1487 TCCGGA [AT]^CCGG[AT]
2628 ACCGGA
BsrDI 922 CATTGC GCAATG(2/0)
3797 CATTGC
BstXI 34 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
363 CCAGTGAGCTGG
CfoI 1380 GCGC GCG^C
2251 GCGC
HaeII 1379 AGCGCT [GA]GCGC^[CT]
2250 GGCGCT
HhaI 1380 GCGC GCG^C
2251 GCGC
HinP1I 1380 GCGC G^CGC
2251 GCGC
NcoI 912 CCATGG C^CATGG
3059 CCATGG
NspI 1281 ACATGC [GA]CATG^[CT]
3867 ACATGT
PvuII 1424 CAGCTG CAG^CTG
3274 CAGCTG
XmnI 623 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3532 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII