################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BciVI BfrI BlnI BsiWI BsmBI BsmI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 1007 CGCG CG^CG AccIII 1487 TCCGGA T^CCGGA AcyI 1202 GACGCC G[GA]^CG[CT]C AocI 3335 CCTGAGG CC^T[ACGT]AGG Aor51HI 1379 AGCGCT AGC^GCT AseI 1874 ATTAAT AT^TAAT AsnI 1874 ATTAAT AT^TAAT BanI 102 GGCACC G^G[CT][GA]CC BbrPI 3386 CACGTG CAC^GTG BcgI 1266 GCAAAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2999 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2999 GCTCAGC GC^T[ACGT]AGC BsaHI 1202 GACGCC G[GA]^CG[CT]C BseAI 1487 TCCGGA T^CCGGA BsiEI 895 CGGTCG CG[GA][CT]^CG Bsp1407I 4360 TGTACA T^GTACA BspEI 1487 TCCGGA T^CCGGA BspHI 1248 TCATGA T^CATGA BspLU11I 3867 ACATGT A^CATGT BsrBI 884 GAGCGG CCGCTC(-3/-3) BsrFI 1352 GCCGGT [GA]^CCGG[CT] BsrGI 4360 TGTACA T^GTACA BssSI 2286 CACGAG CACGAG(-5/-1) BstAPI 446 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1007 CGCG CG^CG Bsu36I 3335 CCTGAGG CC^T[ACGT]AGG CelII 2999 GCTCAGC GC^T[ACGT]AGC Cfr10I 1352 GCCGGT [GA]^CCGG[CT] EaeI 955 TGGCCG [CT]^GGCC[GA] Eco47III 1379 AGCGCT AGC^GCT Eco81I 3335 CCTGAGG CC^T[ACGT]AGG EcoRI 2645 GAATTC G^AATTC FauI 725 GCGGG CCCGC(4/6) HgaI 1202 GACGC GACGC(5/10) Hin1I 1202 GACGCC G[GA]^CG[CT]C HindIII 3295 AAGCTT A^AGCTT HpaI 2270 GTTAAC GTT^AAC MroI 1487 TCCGGA T^CCGGA MvnI 1007 CGCG CG^CG PmaCI 3386 CACGTG CAC^GTG PmlI 3386 CACGTG CAC^GTG PshBI 1874 ATTAAT AT^TAAT RcaI 1248 TCATGA T^CATGA SacI 2354 GAGCTC GAGCT^C SbfI 2380 CCTGCAGG CCTGCA^GG ScaI 2636 AGTACT AGT^ACT SexAI 2521 ACCTGGT A^CC[AT]GGT SpeI 2976 ACTAGT A^CTAGT Sse8387I 2380 CCTGCAGG CCTGCA^GG SspBI 4360 TGTACA T^GTACA StuI 1020 AGGCCT AGG^CCT TaqI 1418 TCGA T^CGA TthHB8I 1418 TCGA T^CGA XbaI 3895 TCTAGA T^CTAGA XcmI 531 CCAGCAAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 329 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 421 CAGAAGCTG ApaI 385 GGGCCC GGGCC^C 3308 GGGCCC Asp700I 623 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3532 GAATAATTTC BbvCI 520 GCTGAGG CCTCAGC(-5/-2) 649 GCTGAGG BglII 1086 AGATCT A^GATCT 1341 AGATCT BsaAI 1178 TACGTG [CT]AC^GT[GA] 3386 CACGTG BsaWI 1487 TCCGGA [AT]^CCGG[AT] 2628 ACCGGA BsrDI 922 CATTGC GCAATG(2/0) 3797 CATTGC BstXI 34 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 363 CCAGTGAGCTGG CfoI 1380 GCGC GCG^C 2251 GCGC HaeII 1379 AGCGCT [GA]GCGC^[CT] 2250 GGCGCT HhaI 1380 GCGC GCG^C 2251 GCGC HinP1I 1380 GCGC G^CGC 2251 GCGC NcoI 912 CCATGG C^CATGG 3059 CCATGG NspI 1281 ACATGC [GA]CATG^[CT] 3867 ACATGT PvuII 1424 CAGCTG CAG^CTG 3274 CAGCTG XmnI 623 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3532 GAATAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII