##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsiWI BsmBI Bsp1407I BspDI
BsrGI BstEII BstPI BstXI CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
FbaI FseI FspI HaeII Hin1I
HpaI KasI KspI MamI MluI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1631 GGTACC G^GTACC
AccIII 722 TCCGGA T^CCGGA
AflIII 2809 ACATGT A^C[GA][CT]GT
ApaI 243 GGGCCC GGGCC^C
Asp700I 998 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1631 GGTACC G^GTACC
AvaI 193 CTCGGG C^[CT]CG[GA]G
BalI 3030 TGGCCA TGG^CCA
BcgI 385 CGAGGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1204 GGATAC GTATCC(6/5)
BsaI 3727 GAGACC GGTCTC(1/5)
BsaWI 722 TCCGGA [AT]^CCGG[AT]
BseAI 722 TCCGGA T^CCGGA
BsiEI 3 CGACCG CG[GA][CT]^CG
BsoBI 193 CTCGGG C^[CT]CG[GA]G
BspEI 722 TCCGGA T^CCGGA
BspHI 770 TCATGA T^CATGA
BspLU11I 2809 ACATGT A^CATGT
BsrDI 2855 GCAATG GCAATG(2/0)
BsrFI 1208 ACCGGC [GA]^CCGG[CT]
BssHII 395 GCGCGC G^CGCGC
BssSI 551 CACGAG CACGAG(-5/-1)
Bst1107I 530 GTATAC GTA^TAC
BstAPI 139 GCAACGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2423 TTCGAA TT^CGAA
BstZ17I 530 GTATAC GTA^TAC
Cfr10I 1208 ACCGGC [GA]^CCGG[CT]
EcoRV 2074 GATATC GAT^ATC
EcoT22I 2858 ATGCAT ATGCA^T
KpnI 1631 GGTACC GGTAC^C
MfeI 131 CAATTG C^AATTG
MluNI 3030 TGGCCA TGG^CCA
MroI 722 TCCGGA T^CCGGA
MscI 3030 TGGCCA TGG^CCA
MunI 131 CAATTG C^AATTG
NdeI 2006 CATATG CA^TATG
NsiI 2858 ATGCAT ATGCA^T
NspI 2809 ACATGT [GA]CATG^[CT]
NspV 2423 TTCGAA TT^CGAA
PpuMI 110 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 990 CAGCTG CAG^CTG
RcaI 770 TCATGA T^CATGA
SacI 1130 GAGCTC GAGCT^C
SalI 518 GTCGAC G^TCGAC
SapI 3886 GCTCTTC GCTCTTC(1/4)
SfuI 2423 TTCGAA TT^CGAA
SpeI 902 ACTAGT A^CTAGT
XmnI 998 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 518 GTCGAC GT^[AC][GT]AC
530 GTATAC
AocI 2923 CCTGAGG CC^T[ACGT]AGG
3681 CCTGAGG
AseI 1534 ATTAAT AT^TAAT
3216 ATTAAT
AsnI 1534 ATTAAT AT^TAAT
3216 ATTAAT
AspHI 1130 GAGCTC G[AT]GC[AT]^C
1646 GAGCAC
BamHI 1822 GGATCC G^GATCC
1860 GGATCC
BglII 321 AGATCT A^GATCT
1999 AGATCT
BpmI 49 CTGGAG CTGGAG(16/14)
2803 CTGGAG
BsgI 186 CTGCAC GTGCAG(16/14)
3828 CTGCAC
BsiHKAI 1130 GAGCTC G[AT]GC[AT]^C
1646 GAGCAC
BsmI 1581 GAATGC GAATGC(1/-1)
1361 GCATTC
Bsu36I 2923 CCTGAGG CC^T[ACGT]AGG
3681 CCTGAGG
DraII 110 GGGTCCC [GA]G^G[ACGT]CC[CT]
242 AGGGCCC
DraIII 1571 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3839 CACTTTGTG
DsaI 6 CCGTGG C^C[GA][CT]GG
252 CCACGG
Eco81I 2923 CCTGAGG CC^T[ACGT]AGG
3681 CCTGAGG
EcoO109I 110 GGGTCCC [GA]G^G[ACGT]CC[CT]
242 AGGGCCC
EcoRI 2426 GAATTC G^AATTC
3199 GAATTC
FauI 180 GCGGG CCCGC(4/6)
486 GCGGG
HgaI 3235 GACGC GACGC(5/10)
212 GCGTC
HindIII 1189 AAGCTT A^AGCTT
3247 AAGCTT
MslI 373 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1960 CACATTGATG
PshBI 1534 ATTAAT AT^TAAT
3216 ATTAAT
SnaBI 868 TACGTA TAC^GTA
3869 TACGTA
XbaI 1996 TCTAGA T^CTAGA
2491 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII