##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AviII       BamHI       BbeI        BbrPI       
BciVI       BfrI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       FseI        FspI        HaeII       
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MluI        MroI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         1396  GTCTAC               GT^[AC][GT]AC
AlwNI        3524  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          436  CCCGAG               C^[CT]CG[GA]G
BcgI         3619  CGAAAAGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          405  TGATCA               T^GATCA
BsaI         4303  GAGACC               GGTCTC(1/5)
BsgI         4464  CTGCAC               GTGCAG(16/14)
BsoBI         436  CCCGAG               C^[CT]CG[GA]G
BstBI        1576  TTCGAA               TT^CGAA
BstEII        507  GGTGACC              G^GT[ACGT]ACC
BstPI         507  GGTGACC              G^GT[ACGT]ACC
BstXI        4253  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         3877  TTTAAA               TTT^AAA
DraII         364  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI         3763  CCATGG               C^C[GA][CT]GG
EcoO109I      364  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       507  GGTGACC              G^GT[ACGT]ACC
EcoRV        1040  GATATC               GAT^ATC
FauI         1012  GCGGG                CCCGC(4/6)
FbaI          405  TGATCA               T^GATCA
HincII        790  GTTGAC               GT[CT]^[GA]AC
HindII        790  GTTGAC               GT[CT]^[GA]AC
MaeII         873  ACGT                 A^CGT
MfeI         4333  CAATTG               C^AATTG
MspA1I        134  CAGCTG               C[AC]G^C[GT]G
MunI         4333  CAATTG               C^AATTG
NcoI         3763  CCATGG               C^CATGG
NspV         1576  TTCGAA               TT^CGAA
PflMI        1108  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         2860  GACTC                GAGTC(4/5)
PvuII         134  CAGCTG               CAG^CTG
SacI          604  GAGCTC               GAGCT^C
ScaI         3990  AGTACT               AGT^ACT
SfuI         1576  TTCGAA               TT^CGAA
SmlI          186  CTTGAG               C^T[CT][GA]AG
SspI          932  AATATT               AAT^ATT
Van91I       1108  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          268  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AspHI         538  GTGCTC               G[AT]GC[AT]^C
              604  GAGCTC              
AspI           16  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTGTGTC           
AvaII         844  GGACC                G^G[AT]CC
             4249  GGTCC               
AvrII         533  CCTAGG               C^CTAGG
             3510  CCTAGG              
BalI          807  TGGCCA               TGG^CCA
             3312  TGGCCA              
BanI         3968  GGTGCC               G^G[CT][GA]CC
             4374  GGTGCC              
BbvI         1345  GCAGC                GCAGC(8/12)
             2226  GCAGC               
BcnI         4359  CCGGG                CC^[GC]GG
             4386  CCCGG               
BlnI          533  CCTAGG               C^CTAGG
             3510  CCTAGG              
BsiHKAI       538  GTGCTC               G[AT]GC[AT]^C
              604  GAGCTC              
BstYI         665  GGATCT               [GA]^GATC[CT]
              858  GGATCT              
Cac8I        1893  GCAAGC               GC[ACGT]^[ACGT]GC
             4042  GCAGGC              
CfoI           76  GCGC                 GCG^C
             4229  GCGC                
DraIII        756  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1109  CACACAGTG           
EaeI          807  TGGCCA               [CT]^GGCC[GA]
             3312  TGGCCA              
HhaI           76  GCGC                 GCG^C
             4229  GCGC                
HinP1I         76  GCGC                 G^CGC
             4229  GCGC                
HindIII      1292  AAGCTT               A^AGCTT
             1318  AAGCTT              
MflI          665  GGATCT               [GA]^GATC[CT]
              858  GGATCT              
MluNI         807  TGGCCA               TGG^CCA
             3312  TGGCCA              
MscI          807  TGGCCA               TGG^CCA
             3312  TGGCCA              
NciI         4359  CCGGG                CC^[GC]GG
             4386  CCCGG               
NdeI         3104  CATATG               CA^TATG
             3380  CATATG              
PflFI          16  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTGTGTC           
PstI         1343  CTGCAG               CTGCA^G
             4123  CTGCAG              
StuI          378  AGGCCT               AGG^CCT
             3403  AGGCCT              
TseI         1345  GCAGC                G^C[AT]GC
             2226  GCAGC               
Tth111I        16  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTGTGTC           
XhoII         665  GGATCT               [GA]^GATC[CT]
              858  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApoI        AseI        AsnI        
Asp700I     BanII       BbsI        BbvCI       BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrDI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       Bsu36I      
Cfr13I      DdeI        DpnII       EarI        Eco81I      
EcoRI       EcoRII      EcoT14I     EcoT22I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MamI        MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        PshBI       
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StyI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI