################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII BamHI BbeI BbrPI BciVI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1396 GTCTAC GT^[AC][GT]AC AlwNI 3524 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 436 CCCGAG C^[CT]CG[GA]G BcgI 3619 CGAAAAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 405 TGATCA T^GATCA BsaI 4303 GAGACC GGTCTC(1/5) BsgI 4464 CTGCAC GTGCAG(16/14) BsoBI 436 CCCGAG C^[CT]CG[GA]G BstBI 1576 TTCGAA TT^CGAA BstEII 507 GGTGACC G^GT[ACGT]ACC BstPI 507 GGTGACC G^GT[ACGT]ACC BstXI 4253 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 3877 TTTAAA TTT^AAA DraII 364 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 3763 CCATGG C^C[GA][CT]GG EcoO109I 364 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 507 GGTGACC G^GT[ACGT]ACC EcoRV 1040 GATATC GAT^ATC FauI 1012 GCGGG CCCGC(4/6) FbaI 405 TGATCA T^GATCA HincII 790 GTTGAC GT[CT]^[GA]AC HindII 790 GTTGAC GT[CT]^[GA]AC MaeII 873 ACGT A^CGT MfeI 4333 CAATTG C^AATTG MspA1I 134 CAGCTG C[AC]G^C[GT]G MunI 4333 CAATTG C^AATTG NcoI 3763 CCATGG C^CATGG NspV 1576 TTCGAA TT^CGAA PflMI 1108 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 2860 GACTC GAGTC(4/5) PvuII 134 CAGCTG CAG^CTG SacI 604 GAGCTC GAGCT^C ScaI 3990 AGTACT AGT^ACT SfuI 1576 TTCGAA TT^CGAA SmlI 186 CTTGAG C^T[CT][GA]AG SspI 932 AATATT AAT^ATT Van91I 1108 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 268 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 538 GTGCTC G[AT]GC[AT]^C 604 GAGCTC AspI 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 912 GACTGTGTC AvaII 844 GGACC G^G[AT]CC 4249 GGTCC AvrII 533 CCTAGG C^CTAGG 3510 CCTAGG BalI 807 TGGCCA TGG^CCA 3312 TGGCCA BanI 3968 GGTGCC G^G[CT][GA]CC 4374 GGTGCC BbvI 1345 GCAGC GCAGC(8/12) 2226 GCAGC BcnI 4359 CCGGG CC^[GC]GG 4386 CCCGG BlnI 533 CCTAGG C^CTAGG 3510 CCTAGG BsiHKAI 538 GTGCTC G[AT]GC[AT]^C 604 GAGCTC BstYI 665 GGATCT [GA]^GATC[CT] 858 GGATCT Cac8I 1893 GCAAGC GC[ACGT]^[ACGT]GC 4042 GCAGGC CfoI 76 GCGC GCG^C 4229 GCGC DraIII 756 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1109 CACACAGTG EaeI 807 TGGCCA [CT]^GGCC[GA] 3312 TGGCCA HhaI 76 GCGC GCG^C 4229 GCGC HinP1I 76 GCGC G^CGC 4229 GCGC HindIII 1292 AAGCTT A^AGCTT 1318 AAGCTT MflI 665 GGATCT [GA]^GATC[CT] 858 GGATCT MluNI 807 TGGCCA TGG^CCA 3312 TGGCCA MscI 807 TGGCCA TGG^CCA 3312 TGGCCA NciI 4359 CCGGG CC^[GC]GG 4386 CCCGG NdeI 3104 CATATG CA^TATG 3380 CATATG PflFI 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 912 GACTGTGTC PstI 1343 CTGCAG CTGCA^G 4123 CTGCAG StuI 378 AGGCCT AGG^CCT 3403 AGGCCT TseI 1345 GCAGC G^C[AT]GC 2226 GCAGC Tth111I 16 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 912 GACTGTGTC XhoII 665 GGATCT [GA]^GATC[CT] 858 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AocI ApoI AseI AsnI Asp700I BanII BbsI BbvCI BfaI BmyI BpmI BpuAI BsaBI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI Bsu36I Cfr13I DdeI DpnII EarI Eco81I EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI PshBI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XmnI