##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI FspI HaeII HgaI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 911 GACGTC GACGT^C
AclI 1259 AACGTT AA^CGTT
AcyI 911 GACGTC G[GA]^CG[CT]C
AflIII 1743 ACATGT A^C[GA][CT]GT
Asp700I 1851 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 870 GTGCTC G[AT]GC[AT]^C
AvaI 3058 CCCGGG C^[CT]CG[GA]G
BanI 2422 GGCACC G^G[CT][GA]CC
BglII 3184 AGATCT A^GATCT
BsaHI 911 GACGTC G[GA]^CG[CT]C
BsaI 824 GAGACC GGTCTC(1/5)
BsiHKAI 870 GTGCTC G[AT]GC[AT]^C
BsmBI 3232 GAGACG CGTCTC(1/5)
BsmI 455 GAATGC GAATGC(1/-1)
BsoBI 3058 CCCGGG C^[CT]CG[GA]G
BspLU11I 1743 ACATGT A^CATGT
BsrFI 2207 GCCGGC [GA]^CCGG[CT]
BssSI 2881 CTCGTG CACGAG(-5/-1)
Cfr10I 2207 GCCGGC [GA]^CCGG[CT]
EcoNI 2072 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2018 ATGCAT ATGCA^T
Hin1I 911 GACGTC G[GA]^CG[CT]C
HincII 900 GTCAAC GT[CT]^[GA]AC
HindII 900 GTCAAC GT[CT]^[GA]AC
NaeI 2207 GCCGGC GCC^GGC
NgoMIV 2207 GCCGGC G^CCGGC
NsiI 2018 ATGCAT ATGCA^T
Psp1406I 1259 AACGTT AA^CGTT
SmaI 3058 CCCGGG CCC^GGG
SmlI 3188 CTTGAG C^T[CT][GA]AG
XmaCI 3058 CCCGGG C^CCGGG
XmaI 3058 CCCGGG C^CCGGG
XmnI 1851 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1566 GTCTAC GT^[AC][GT]AC
2130 GTAGAC
BalI 1671 TGGCCA TGG^CCA
1721 TGGCCA
BamHI 446 GGATCC G^GATCC
2299 GGATCC
BanII 2276 GAGCCC G[GA]GC[CT]^C
3055 GAGCCC
BbvCI 999 CCTCAGC CCTCAGC(-5/-2)
1140 CCTCAGC
BsaWI 572 ACCGGA [AT]^CCGG[AT]
1562 TCCGGT
Bsp1407I 1331 TGTACA T^GTACA
2460 TGTACA
BspHI 479 TCATGA T^CATGA
1688 TCATGA
BsrDI 595 CATTGC GCAATG(2/0)
833 CATTGC
BsrGI 1331 TGTACA T^GTACA
2460 TGTACA
BstAPI 786 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1702 GCAGCGTTTGC
BstEII 842 GGTGACC G^GT[ACGT]ACC
2951 GGTAACC
BstPI 842 GGTGACC G^GT[ACGT]ACC
2951 GGTAACC
BstXI 832 CCATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1078 CCAAGCCTGTGG
CfoI 2947 GCGC GCG^C
3009 GCGC
EaeI 1671 TGGCCA [CT]^GGCC[GA]
1721 TGGCCA
EcoO65I 842 GGTGACC G^GT[ACGT]ACC
2951 GGTAACC
EcoRI 1205 GAATTC G^AATTC
2370 GAATTC
FauI 3027 CCCGC CCCGC(4/6)
2873 GCGGG
HhaI 2947 GCGC GCG^C
3009 GCGC
HinP1I 2947 GCGC G^CGC
3009 GCGC
MfeI 1879 CAATTG C^AATTG
2437 CAATTG
MluNI 1671 TGGCCA TGG^CCA
1721 TGGCCA
MscI 1671 TGGCCA TGG^CCA
1721 TGGCCA
MunI 1879 CAATTG C^AATTG
2437 CAATTG
NcoI 1267 CCATGG C^CATGG
1860 CCATGG
PpuMI 1641 GGGACCC [GA]G^G[AT]CC[CT]
3428 AGGTCCC
RcaI 479 TCATGA T^CATGA
1688 TCATGA
SspBI 1331 TGTACA T^GTACA
2460 TGTACA
TaqI 1073 TCGA T^CGA
2113 TCGA
TfiI 2093 GATTC G^A[AT]TC
3411 GAATC
TthHB8I 1073 TCGA T^CGA
2113 TCGA
XbaI 1508 TCTAGA T^CTAGA
2335 TCTAGA
XcmI 2588 CCACAGCCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2708 CCAAAGAGTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII