##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
ApaLI AscI AspI AviII BbeI
BbrPI BcgI BciVI BcnI BfrI
BglII BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI ClaI
CpoI DrdI EcoRV EcoT22I FseI
FspI KasI KspI MfeI MluI
MroI MunI NaeI NarI NciI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I SspBI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2363 GGTACC G^GTACC
AccI 3934 GTATAC GT^[AC][GT]AC
AccII 352 CGCG CG^CG
AclI 3569 AACGTT AA^CGTT
AcyI 71 GGCGTC G[GA]^CG[CT]C
AocI 4013 CCTGAGG CC^T[ACGT]AGG
ApaI 1931 GGGCCC GGGCC^C
Asp718I 2363 GGTACC G^GTACC
AvrII 2377 CCTAGG C^CTAGG
BalI 780 TGGCCA TGG^CCA
BbsI 1940 GAAGAC GAAGAC(2/6)
BbvCI 3620 GCTGAGG CCTCAGC(-5/-2)
BclI 1587 TGATCA T^GATCA
BlnI 2377 CCTAGG C^CTAGG
BlpI 4515 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4515 GCTTAGC GC^T[ACGT]AGC
BpuAI 1940 GAAGAC GAAGAC(2/6)
BsaHI 71 GGCGTC G[GA]^CG[CT]C
BsaWI 1734 TCCGGT [AT]^CCGG[AT]
BspHI 450 TCATGA T^CATGA
BspLU11I 1205 ACATGT A^CATGT
BsrFI 1431 ACCGGC [GA]^CCGG[CT]
Bst1107I 3934 GTATAC GTA^TAC
BstEII 1459 GGTGACC G^GT[ACGT]ACC
BstPI 1459 GGTGACC G^GT[ACGT]ACC
BstUI 352 CGCG CG^CG
BstZ17I 3934 GTATAC GTA^TAC
Bsu36I 4013 CCTGAGG CC^T[ACGT]AGG
CelII 4515 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1431 ACCGGC [GA]^CCGG[CT]
DraIII 1454 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4013 CCTGAGG CC^T[ACGT]AGG
EcoNI 3506 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1459 GGTGACC G^GT[ACGT]ACC
FbaI 1587 TGATCA T^GATCA
HgaI 72 GCGTC GACGC(5/10)
Hin1I 71 GGCGTC G[GA]^CG[CT]C
KpnI 2363 GGTACC GGTAC^C
MluNI 780 TGGCCA TGG^CCA
MscI 780 TGGCCA TGG^CCA
MspA1I 268 CCGCTG C[AC]G^C[GT]G
MvnI 352 CGCG CG^CG
NdeI 663 CATATG CA^TATG
PaeR7I 1815 CTCGAG C^TCGAG
PflMI 1722 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3569 AACGTT AA^CGTT
RcaI 450 TCATGA T^CATGA
SnaBI 3403 TACGTA TAC^GTA
SphI 3641 GCATGC GCATG^C
SspI 1346 AATATT AAT^ATT
Van91I 1722 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1815 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 612 ACACGT A^C[GA][CT]GT
1205 ACATGT
AhdI 18 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
801 GACTGACAGTC
Aor51HI 952 AGCGCT AGC^GCT
1811 AGCGCT
Asp700I 3524 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3580 GAACATATTC
AspEI 18 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
801 GACTGACAGTC
AvaI 1815 CTCGAG C^[CT]CG[GA]G
2498 CCCGAG
BamHI 1731 GGATCC G^GATCC
2645 GGATCC
BsaAI 1840 CACGTA [CT]AC^GT[GA]
3403 TACGTA
BsaBI 997 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4225 GATACATATC
BsaI 981 GAGACC GGTCTC(1/5)
1030 GGTCTC
BsgI 1829 CTGCAC GTGCAG(16/14)
3392 CTGCAC
BsiEI 210 CGGCCG CG[GA][CT]^CG
1987 CGGCCG
BsoBI 1815 CTCGAG C^[CT]CG[GA]G
2498 CCCGAG
CfoI 953 GCGC GCG^C
1812 GCGC
DraI 3886 TTTAAA TTT^AAA
3900 TTTAAA
EagI 210 CGGCCG C^GGCCG
1987 CGGCCG
Eam1105I 18 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
801 GACTGACAGTC
EclXI 210 CGGCCG C^GGCCG
1987 CGGCCG
Eco47III 952 AGCGCT AGC^GCT
1811 AGCGCT
Eco52I 210 CGGCCG C^GGCCG
1987 CGGCCG
EcoRI 391 GAATTC G^AATTC
3380 GAATTC
FauI 1929 GCGGG CCCGC(4/6)
2882 GCGGG
HaeII 952 AGCGCT [GA]GCGC^[CT]
1811 AGCGCT
HhaI 953 GCGC GCG^C
1812 GCGC
HinP1I 953 GCGC G^CGC
1812 GCGC
HpaI 4075 GTTAAC GTT^AAC
4422 GTTAAC
MamI 997 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4225 GATACATATC
NcoI 855 CCATGG C^CATGG
3771 CCATGG
NheI 3168 GCTAGC G^CTAGC
3590 GCTAGC
PstI 1613 CTGCAG CTGCA^G
2856 CTGCAG
SexAI 19 ACCTGGT A^CC[AT]GGT
3246 ACCTGGT
SpeI 3879 ACTAGT A^CTAGT
4333 ACTAGT
StuI 460 AGGCCT AGG^CCT
2380 AGGCCT
XbaI 3965 TCTAGA T^CTAGA
4233 TCTAGA
XmnI 3524 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3580 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII