##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AflII AgeI AhdI
AscI AspEI BamHI BbvCI BclI
BfrI BglII BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DraIII
Eam1105I EcoO65I EcoRV EcoT22I FbaI
FseI MamI MluI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RcaI
SalI SapI SbfI ScaI SexAI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp45I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1303 GGTACC G^GTACC
AclI 2561 AACGTT AA^CGTT
AlwI 3465 GGATC GGATC(4/5)
AocI 2841 CCTGAGG CC^T[ACGT]AGG
AseI 3106 ATTAAT AT^TAAT
AsnI 3106 ATTAAT AT^TAAT
Asp718I 1303 GGTACC G^GTACC
AspI 1618 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3094 CCTAGG C^CTAGG
BbrPI 3472 CACGTG CAC^GTG
BciVI 2571 GGATAC GTATCC(6/5)
BlnI 3094 CCTAGG C^CTAGG
BlpI 1470 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1470 GCTGAGC GC^T[ACGT]AGC
BstYI 3465 GGATCT [GA]^GATC[CT]
Bsu36I 2841 CCTGAGG CC^T[ACGT]AGG
CelII 1470 GCTGAGC GC^T[ACGT]AGC
CpoI 2259 CGGTCCG CG^G[AT]CCG
DrdI 1347 GACCTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2841 CCTGAGG CC^T[ACGT]AGG
EcoRI 629 GAATTC G^AATTC
HindIII 3286 AAGCTT A^AGCTT
HpaI 3580 GTTAAC GTT^AAC
KpnI 1303 GGTACC GGTAC^C
MfeI 3631 CAATTG C^AATTG
MflI 3465 GGATCT [GA]^GATC[CT]
MunI 3631 CAATTG C^AATTG
NcoI 2519 CCATGG C^CATGG
PflFI 1618 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3472 CACGTG CAC^GTG
PmlI 3472 CACGTG CAC^GTG
PshBI 3106 ATTAAT AT^TAAT
Psp1406I 2561 AACGTT AA^CGTT
PstI 191 CTGCAG CTGCA^G
RsrII 2259 CGGTCCG CG^G[AT]CCG
SacI 216 GAGCTC GAGCT^C
SphI 549 GCATGC GCATG^C
StuI 2036 AGGCCT AGG^CCT
Tth111I 1618 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2629 TCTAGA T^CTAGA
XcmI 1628 CCAAGCCCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3465 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 2043 TCCGGA T^CCGGA
2508 TCCGGA
Alw44I 2338 GTGCAC G^TGCAC
2491 GTGCAC
AlwNI 346 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1177 CAGACGCTG
ApaLI 2338 GTGCAC G^TGCAC
2491 GTGCAC
Asp700I 1653 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1740 GAAGGACTTC
AviII 1484 TGCGCA TGC^GCA
2024 TGCGCA
BalI 1712 TGGCCA TGG^CCA
2522 TGGCCA
BbsI 1341 GTCTTC GAAGAC(2/6)
3318 GTCTTC
BcgI 624 GCATCGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
905 GCAGCCGCTTCG
BpuAI 1341 GTCTTC GAAGAC(2/6)
3318 GTCTTC
BsaAI 2423 CACGTA [CT]AC^GT[GA]
3472 CACGTG
BsaI 1826 GAGACC GGTCTC(1/5)
2783 GAGACC
BseAI 2043 TCCGGA T^CCGGA
2508 TCCGGA
BsgI 1554 CTGCAC GTGCAG(16/14)
2544 CTGCAC
BsiEI 685 CGGCCG CG[GA][CT]^CG
1749 CGGCCG
BsmBI 1079 CGTCTC CGTCTC(1/5)
1623 CGTCTC
BspEI 2043 TCCGGA T^CCGGA
2508 TCCGGA
BsrBI 688 CCGCTC CCGCTC(-3/-3)
2276 CCGCTC
BssHII 647 GCGCGC G^CGCGC
2069 GCGCGC
EagI 685 CGGCCG C^GGCCG
1749 CGGCCG
EclXI 685 CGGCCG C^GGCCG
1749 CGGCCG
Eco52I 685 CGGCCG C^GGCCG
1749 CGGCCG
EcoNI 421 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
468 CCTTCCCCAGG
FspI 1484 TGCGCA TGC^GCA
2024 TGCGCA
MluNI 1712 TGGCCA TGG^CCA
2522 TGGCCA
MroI 2043 TCCGGA T^CCGGA
2508 TCCGGA
MscI 1712 TGGCCA TGG^CCA
2522 TGGCCA
NruI 225 TCGCGA TCG^CGA
2741 TCGCGA
NspI 549 GCATGC [GA]CATG^[CT]
1880 ACATGC
PvuII 653 CAGCTG CAG^CTG
1908 CAGCTG
SfcI 191 CTGCAG C^T[GA][CT]AG
3575 CTGTAG
SfiI 976 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2646 GGCCCAGGGGGCC
TfiI 2512 GATTC G^A[AT]TC
3545 GAATC
XmnI 1653 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1740 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Aor51HI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfoI
SgrAI SmaI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI