##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
BcgI BclI BfrI BglII BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI HincII HindII HindIII
HpaI KspI MamI MluI NcoI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 3186 GGTACC G^GTACC
AccII 2139 CGCG CG^CG
AccIII 641 TCCGGA T^CCGGA
AcsI 637 GAATTC [GA]^AATT[CT]
AcyI 2213 GGCGCC G[GA]^CG[CT]C
AocI 2847 CCTCAGG CC^T[ACGT]AGG
ApaI 2518 GGGCCC GGGCC^C
ApoI 637 GAATTC [GA]^AATT[CT]
Asp718I 3186 GGTACC G^GTACC
AvrII 3566 CCTAGG C^CTAGG
BalI 2751 TGGCCA TGG^CCA
BamHI 1439 GGATCC G^GATCC
BbeI 2213 GGCGCC GGCGC^C
BbrPI 1319 CACGTG CAC^GTG
BciVI 1776 GGATAC GTATCC(6/5)
BlnI 3566 CCTAGG C^CTAGG
BsaAI 1319 CACGTG [CT]AC^GT[GA]
BsaHI 2213 GGCGCC G[GA]^CG[CT]C
BsaWI 641 TCCGGA [AT]^CCGG[AT]
BseAI 641 TCCGGA T^CCGGA
BsmBI 949 CGTCTC CGTCTC(1/5)
BspEI 641 TCCGGA T^CCGGA
BsrBI 1806 CCGCTC CCGCTC(-3/-3)
BstEII 3238 GGTGACC G^GT[ACGT]ACC
BstPI 3238 GGTGACC G^GT[ACGT]ACC
BstUI 2139 CGCG CG^CG
Bsu36I 2847 CCTCAGG CC^T[ACGT]AGG
DrdI 3241 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2847 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3238 GGTGACC G^GT[ACGT]ACC
EcoRI 637 GAATTC G^AATTC
HgaI 3736 GACGC GACGC(5/10)
Hin1I 2213 GGCGCC G[GA]^CG[CT]C
KasI 2213 GGCGCC G^GCGCC
KpnI 3186 GGTACC GGTAC^C
MfeI 3196 CAATTG C^AATTG
MluNI 2751 TGGCCA TGG^CCA
MroI 641 TCCGGA T^CCGGA
MscI 2751 TGGCCA TGG^CCA
MunI 3196 CAATTG C^AATTG
MvnI 2139 CGCG CG^CG
NaeI 2450 GCCGGC GCC^GGC
NarI 2213 GGCGCC GG^CGCC
NdeI 2986 CATATG CA^TATG
NgoMIV 2450 GCCGGC G^CCGGC
NheI 2127 GCTAGC G^CTAGC
PmaCI 1319 CACGTG CAC^GTG
PmlI 1319 CACGTG CAC^GTG
PshAI 1374 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 4003 ACCAGGT A^CC[AT]GGT
SfiI 2752 GGCCAGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2213 GGCGCC GGC^GCC
SmaI 1865 CCCGGG CCC^GGG
XmaCI 1865 CCCGGG C^CCGGG
XmaI 1865 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 119 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
3719 CAGAGGCTG
AviII 1223 TGCGCA TGC^GCA
1678 TGCGCA
BbsI 1062 GAAGAC GAAGAC(2/6)
1800 GAAGAC
BpuAI 1062 GAAGAC GAAGAC(2/6)
1800 GAAGAC
BspMI 1409 ACCTGC ACCTGC(4/8)
2979 GCAGGT
BsrDI 1220 CATTGC GCAATG(2/0)
2133 CATTGC
BsrFI 2412 ACCGGC [GA]^CCGG[CT]
2450 GCCGGC
Cfr10I 2412 ACCGGC [GA]^CCGG[CT]
2450 GCCGGC
EaeI 2044 CGGCCA [CT]^GGCC[GA]
2751 TGGCCA
EcoNI 269 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3561 CCTTGCCTAGG
EcoT22I 470 ATGCAT ATGCA^T
706 ATGCAT
FspI 1223 TGCGCA TGC^GCA
1678 TGCGCA
HaeII 1157 GGCGCT [GA]GCGC^[CT]
2213 GGCGCC
MaeII 1320 ACGT A^CGT
3887 ACGT
NsiI 470 ATGCAT ATGCA^T
706 ATGCAT
PflMI 577 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1509 CCAGTCTGTGG
SapI 199 GAAGAGC GCTCTTC(1/4)
2632 GCTCTTC
TaqI 545 TCGA T^CGA
1755 TCGA
TthHB8I 545 TCGA T^CGA
1755 TCGA
Van91I 577 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1509 CCAGTCTGTGG
XbaI 157 TCTAGA T^CTAGA
3637 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII