##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI AscI Asp718I AspEI
BamHI BbeI BbrPI BbsI BclI
BglII BpuAI BsaBI BsaHI BsiWI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoRV FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2790 TCCGGA T^CCGGA
AcsI 2876 AAATTT [GA]^AATT[CT]
AflII 406 CTTAAG C^TTAAG
ApoI 2876 AAATTT [GA]^AATT[CT]
AseI 231 ATTAAT AT^TAAT
AsnI 231 ATTAAT AT^TAAT
AspI 11 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1391 TGCGCA TGC^GCA
BcgI 1434 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 306 GGATAC GTATCC(6/5)
BfrI 406 CTTAAG C^TTAAG
BsaAI 138 CACGTA [CT]AC^GT[GA]
BsaI 1031 GGTCTC GGTCTC(1/5)
BseAI 2790 TCCGGA T^CCGGA
BsmBI 1126 GAGACG CGTCTC(1/5)
BspEI 2790 TCCGGA T^CCGGA
BspLU11I 824 ACATGT A^CATGT
BstEII 562 GGTCACC G^GT[ACGT]ACC
BstPI 562 GGTCACC G^GT[ACGT]ACC
DraIII 844 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 412 GACTTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1929 CGGCCG C^GGCCG
EclXI 1929 CGGCCG C^GGCCG
Eco52I 1929 CGGCCG C^GGCCG
EcoO65I 562 GGTCACC G^GT[ACGT]ACC
EcoT22I 2446 ATGCAT ATGCA^T
FspI 1391 TGCGCA TGC^GCA
HgaI 2484 GCGTC GACGC(5/10)
MluI 223 ACGCGT A^CGCGT
MroI 2790 TCCGGA T^CCGGA
NaeI 2210 GCCGGC GCC^GGC
NcoI 423 CCATGG C^CATGG
NgoMIV 2210 GCCGGC G^CCGGC
NsiI 2446 ATGCAT ATGCA^T
PflFI 11 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 231 ATTAAT AT^TAAT
SacI 601 GAGCTC GAGCT^C
SapI 464 GCTCTTC GCTCTTC(1/4)
SphI 1350 GCATGC GCATG^C
Tsp509I 2877 AATT ^AATT
Tth111I 11 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 18 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 224 CGCG CG^CG
2221 CGCG
AfaI 141 GTAC GT^AC
227 GTAC
AflIII 223 ACGCGT A^C[GA][CT]GT
824 ACATGT
Alw44I 841 GTGCAC G^TGCAC
1621 GTGCAC
AocI 1213 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
Aor51HI 1148 AGCGCT AGC^GCT
2833 AGCGCT
ApaI 1437 GGGCCC GGGCC^C
2527 GGGCCC
ApaLI 841 GTGCAC G^TGCAC
1621 GTGCAC
Asp700I 461 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAAGCGCTTC
BalI 1268 TGGCCA TGG^CCA
1553 TGGCCA
BlpI 771 GCTGAGC GC^T[ACGT]AGC
1233 GCTGAGC
Bpu1102I 771 GCTGAGC GC^T[ACGT]AGC
1233 GCTGAGC
BsaWI 1862 ACCGGA [AT]^CCGG[AT]
2790 TCCGGA
BsiEI 1929 CGGCCG CG[GA][CT]^CG
2655 CGACCG
BsmI 2056 GAATGC GAATGC(1/-1)
321 GCATTC
BsrBI 2191 GAGCGG CCGCTC(-3/-3)
2560 GAGCGG
BsrFI 974 ACCGGC [GA]^CCGG[CT]
2210 GCCGGC
BstAPI 42 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2137 GCAGCTGGTGC
BstUI 224 CGCG CG^CG
2221 CGCG
Bsu36I 1213 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
CelII 771 GCTGAGC GC^T[ACGT]AGC
1233 GCTGAGC
Cfr10I 974 ACCGGC [GA]^CCGG[CT]
2210 GCCGGC
Eco47III 1148 AGCGCT AGC^GCT
2833 AGCGCT
Eco81I 1213 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
MluNI 1268 TGGCCA TGG^CCA
1553 TGGCCA
MscI 1268 TGGCCA TGG^CCA
1553 TGGCCA
MvnI 224 CGCG CG^CG
2221 CGCG
NspI 824 ACATGT [GA]CATG^[CT]
1350 GCATGC
PpuMI 656 GGGACCC [GA]G^G[AT]CC[CT]
1887 AGGTCCT
RsaI 141 GTAC GT^AC
227 GTAC
SexAI 12 ACCTGGT A^CC[AT]GGT
845 ACCTGGT
SmaI 386 CCCGGG CCC^GGG
2177 CCCGGG
TfiI 800 GATTC G^A[AT]TC
1468 GAATC
XmaCI 386 CCCGGG C^CCGGG
2177 CCCGGG
XmaI 386 CCCGGG C^CCGGG
2177 CCCGGG
XmnI 461 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII AvrII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII