##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspI        BcgI        BsaBI       BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoO65I     EcoT22I     FseI        
KpnI        KspI        MamI        NdeI        NotI        
NruI        NsiI        NspV        PaeR7I      PflFI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       1935  TCCGGA               T^CCGGA
AcsI         2941  GAATTC               [GA]^AATT[CT]
AflII        3979  CTTAAG               C^TTAAG
AgeI         1652  ACCGGT               A^CCGGT
ApoI         2941  GAATTC               [GA]^AATT[CT]
AseI         4215  ATTAAT               AT^TAAT
AsnI         4215  ATTAAT               AT^TAAT
Asp700I      1878  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        2509  TGCGCA               TGC^GCA
AvrII          91  CCTAGG               C^CTAGG
BamHI         950  GGATCC               G^GATCC
BbrPI         785  CACGTG               CAC^GTG
BbsI         3261  GAAGAC               GAAGAC(2/6)
BclI         2535  TGATCA               T^GATCA
BfrI         3979  CTTAAG               C^TTAAG
BlnI           91  CCTAGG               C^CTAGG
BlpI          695  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      695  GCTCAGC              GC^T[ACGT]AGC
BpuAI        3261  GAAGAC               GAAGAC(2/6)
BsaAI         785  CACGTG               [CT]AC^GT[GA]
BsaI         3713  GGTCTC               GGTCTC(1/5)
BseAI        1935  TCCGGA               T^CCGGA
BspEI        1935  TCCGGA               T^CCGGA
BspHI         821  TCATGA               T^CATGA
BsrDI        1745  GCAATG               GCAATG(2/0)
BssSI        2080  CACGAG               CACGAG(-5/-1)
BstAPI       1278  GCAAGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         695  GCTCAGC              GC^T[ACGT]AGC
EcoRI        2941  GAATTC               G^AATTC
EcoRV        1756  GATATC               GAT^ATC
FbaI         2535  TGATCA               T^GATCA
FspI         2509  TGCGCA               TGC^GCA
MfeI         3695  CAATTG               C^AATTG
MluI          550  ACGCGT               A^CGCGT
MroI         1935  TCCGGA               T^CCGGA
MunI         3695  CAATTG               C^AATTG
NaeI         1459  GCCGGC               GCC^GGC
NgoMIV       1459  GCCGGC               G^CCGGC
NspI         4320  GCATGT               [GA]CATG^[CT]
PacI         4212  TTAATTAA             TTAAT^TAA
PinAI        1652  ACCGGT               A^CCGGT
PmaCI         785  CACGTG               CAC^GTG
PmlI          785  CACGTG               CAC^GTG
PshBI        4215  ATTAAT               AT^TAAT
RcaI          821  TCATGA               T^CATGA
SapI          333  GCTCTTC              GCTCTTC(1/4)
XcmI         1416  CCAGAAGGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1878  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflIII        550  ACGCGT               A^C[GA][CT]GT
              786  ACGTGT              
AocI         1761  CCTGAGG              CC^T[ACGT]AGG
             3209  CCTTAGG             
BciVI        3043  GGATAC               GTATCC(6/5)
             3443  GTATCC              
BglII         671  AGATCT               A^GATCT
             3232  AGATCT              
BsaWI        1652  ACCGGT               [AT]^CCGG[AT]
             1935  TCCGGA              
BsgI          274  CTGCAC               GTGCAG(16/14)
             1314  CTGCAC              
BsiEI         180  CGGCCG               CG[GA][CT]^CG
             1114  CGGCCG              
BsmBI        1475  GAGACG               CGTCTC(1/5)
             2322  GAGACG              
Bsu36I       1761  CCTGAGG              CC^T[ACGT]AGG
             3209  CCTTAGG             
EagI          180  CGGCCG               C^GGCCG
             1114  CGGCCG              
EclXI         180  CGGCCG               C^GGCCG
             1114  CGGCCG              
Eco52I        180  CGGCCG               C^GGCCG
             1114  CGGCCG              
Eco81I       1761  CCTGAGG              CC^T[ACGT]AGG
             3209  CCTTAGG             
HpaI          790  GTTAAC               GTT^AAC
             1582  GTTAAC              
MaeII         786  ACGT                 A^CGT
             2499  ACGT                
NcoI          380  CCATGG               C^CATGG
             3448  CCATGG              
NheI          735  GCTAGC               G^CTAGC
             1983  GCTAGC              
PleI         3328  GACTC                GAGTC(4/5)
             4061  GAGTC               
PvuII         767  CAGCTG               CAG^CTG
             3256  CAGCTG              
SexAI         959  ACCAGGT              A^CC[AT]GGT
             2288  ACCAGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PpuMI       PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI