################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccI AclI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI BcgI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoT22I FseI KpnI KspI MamI NdeI NotI NruI NsiI NspV PaeR7I PflFI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 1935 TCCGGA T^CCGGA AcsI 2941 GAATTC [GA]^AATT[CT] AflII 3979 CTTAAG C^TTAAG AgeI 1652 ACCGGT A^CCGGT ApoI 2941 GAATTC [GA]^AATT[CT] AseI 4215 ATTAAT AT^TAAT AsnI 4215 ATTAAT AT^TAAT Asp700I 1878 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2509 TGCGCA TGC^GCA AvrII 91 CCTAGG C^CTAGG BamHI 950 GGATCC G^GATCC BbrPI 785 CACGTG CAC^GTG BbsI 3261 GAAGAC GAAGAC(2/6) BclI 2535 TGATCA T^GATCA BfrI 3979 CTTAAG C^TTAAG BlnI 91 CCTAGG C^CTAGG BlpI 695 GCTCAGC GC^T[ACGT]AGC Bpu1102I 695 GCTCAGC GC^T[ACGT]AGC BpuAI 3261 GAAGAC GAAGAC(2/6) BsaAI 785 CACGTG [CT]AC^GT[GA] BsaI 3713 GGTCTC GGTCTC(1/5) BseAI 1935 TCCGGA T^CCGGA BspEI 1935 TCCGGA T^CCGGA BspHI 821 TCATGA T^CATGA BsrDI 1745 GCAATG GCAATG(2/0) BssSI 2080 CACGAG CACGAG(-5/-1) BstAPI 1278 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 695 GCTCAGC GC^T[ACGT]AGC EcoRI 2941 GAATTC G^AATTC EcoRV 1756 GATATC GAT^ATC FbaI 2535 TGATCA T^GATCA FspI 2509 TGCGCA TGC^GCA MfeI 3695 CAATTG C^AATTG MluI 550 ACGCGT A^CGCGT MroI 1935 TCCGGA T^CCGGA MunI 3695 CAATTG C^AATTG NaeI 1459 GCCGGC GCC^GGC NgoMIV 1459 GCCGGC G^CCGGC NspI 4320 GCATGT [GA]CATG^[CT] PacI 4212 TTAATTAA TTAAT^TAA PinAI 1652 ACCGGT A^CCGGT PmaCI 785 CACGTG CAC^GTG PmlI 785 CACGTG CAC^GTG PshBI 4215 ATTAAT AT^TAAT RcaI 821 TCATGA T^CATGA SapI 333 GCTCTTC GCTCTTC(1/4) XcmI 1416 CCAGAAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1878 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 550 ACGCGT A^C[GA][CT]GT 786 ACGTGT AocI 1761 CCTGAGG CC^T[ACGT]AGG 3209 CCTTAGG BciVI 3043 GGATAC GTATCC(6/5) 3443 GTATCC BglII 671 AGATCT A^GATCT 3232 AGATCT BsaWI 1652 ACCGGT [AT]^CCGG[AT] 1935 TCCGGA BsgI 274 CTGCAC GTGCAG(16/14) 1314 CTGCAC BsiEI 180 CGGCCG CG[GA][CT]^CG 1114 CGGCCG BsmBI 1475 GAGACG CGTCTC(1/5) 2322 GAGACG Bsu36I 1761 CCTGAGG CC^T[ACGT]AGG 3209 CCTTAGG EagI 180 CGGCCG C^GGCCG 1114 CGGCCG EclXI 180 CGGCCG C^GGCCG 1114 CGGCCG Eco52I 180 CGGCCG C^GGCCG 1114 CGGCCG Eco81I 1761 CCTGAGG CC^T[ACGT]AGG 3209 CCTTAGG HpaI 790 GTTAAC GTT^AAC 1582 GTTAAC MaeII 786 ACGT A^CGT 2499 ACGT NcoI 380 CCATGG C^CATGG 3448 CCATGG NheI 735 GCTAGC G^CTAGC 1983 GCTAGC PleI 3328 GACTC GAGTC(4/5) 4061 GAGTC PvuII 767 CAGCTG CAG^CTG 3256 CAGCTG SexAI 959 ACCAGGT A^CC[AT]GGT 2288 ACCAGGT ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI