##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BclI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FbaI FseI FspI
KpnI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 2758 AACGTT AA^CGTT
AcsI 4101 AAATTC [GA]^AATT[CT]
AflII 464 CTTAAG C^TTAAG
Aor51HI 4694 AGCGCT AGC^GCT
ApoI 4101 AAATTC [GA]^AATT[CT]
BamHI 2751 GGATCC G^GATCC
BbeI 2174 GGCGCC GGCGC^C
BbrPI 770 CACGTG CAC^GTG
BbvCI 4677 GCTGAGG CCTCAGC(-5/-2)
BciVI 684 GGATAC GTATCC(6/5)
BfrI 464 CTTAAG C^TTAAG
BsaAI 770 CACGTG [CT]AC^GT[GA]
BsaBI 685 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2191 CGTCTC CGTCTC(1/5)
BspMI 4073 ACCTGC ACCTGC(4/8)
BstAPI 1636 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3461 GGTTACC G^GT[ACGT]ACC
BstPI 3461 GGTTACC G^GT[ACGT]ACC
BstXI 912 CCAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2181 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4694 AGCGCT AGC^GCT
EcoO65I 3461 GGTTACC G^GT[ACGT]ACC
EcoT22I 152 ATGCAT ATGCA^T
HindIII 1880 AAGCTT A^AGCTT
HpaI 638 GTTAAC GTT^AAC
KasI 2174 GGCGCC G^GCGCC
MamI 685 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2174 GGCGCC GG^CGCC
NsiI 152 ATGCAT ATGCA^T
PflMI 756 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 770 CACGTG CAC^GTG
PmlI 770 CACGTG CAC^GTG
Psp1406I 2758 AACGTT AA^CGTT
SbfI 2447 CCTGCAGG CCTGCA^GG
SfoI 2174 GGCGCC GGC^GCC
SmaI 2306 CCCGGG CCC^GGG
Sse8387I 2447 CCTGCAGG CCTGCA^GG
SspI 618 AATATT AAT^ATT
Van91I 756 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2306 CCCGGG C^CCGGG
XmaI 2306 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1151 GTCTAC GT^[AC][GT]AC
4322 GTCTAC
AspI 3332 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4316 GACACAGTC
BcgI 1573 GCAGCCGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2206 GCAGACGGATCG
Bsp1407I 47 TGTACA T^GTACA
774 TGTACA
BspLU11I 645 ACATGT A^CATGT
1369 ACATGT
BsrDI 237 CATTGC GCAATG(2/0)
1222 CATTGC
BsrGI 47 TGTACA T^GTACA
774 TGTACA
HaeII 2174 GGCGCC [GA]GCGC^[CT]
4694 AGCGCT
HgaI 2330 GACGC GACGC(5/10)
2591 GCGTC
HincII 638 GTTAAC GT[CT]^[GA]AC
4460 GTCAAC
HindII 638 GTTAAC GT[CT]^[GA]AC
4460 GTCAAC
KspI 1858 CCGCGG CCGC^GG
3256 CCGCGG
NaeI 2171 GCCGGC GCC^GGC
2581 GCCGGC
NcoI 2691 CCATGG C^CATGG
4526 CCATGG
NgoMIV 2171 GCCGGC G^CCGGC
2581 GCCGGC
PflFI 3332 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4316 GACACAGTC
SacII 1858 CCGCGG CCGC^GG
3256 CCGCGG
SexAI 1440 ACCTGGT A^CC[AT]GGT
3545 ACCTGGT
SspBI 47 TGTACA T^GTACA
774 TGTACA
Tth111I 3332 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4316 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII