##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AspEI AviII AvrII BamHI BbeI
BbrPI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KspI MamI
MluI MroI MspA1I MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SwaI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3766 GGTACC G^GTACC
AccI 571 GTATAC GT^[AC][GT]AC
AcyI 1756 GGCGTC G[GA]^CG[CT]C
Alw44I 1699 GTGCAC G^TGCAC
AlwNI 1066 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3480 CCTGAGG CC^T[ACGT]AGG
ApaLI 1699 GTGCAC G^TGCAC
Asp718I 3766 GGTACC G^GTACC
BbsI 1545 GAAGAC GAAGAC(2/6)
BbvCI 3599 GCTGAGG CCTCAGC(-5/-2)
BciVI 1144 GTATCC GTATCC(6/5)
BglII 1839 AGATCT A^GATCT
BpuAI 1545 GAAGAC GAAGAC(2/6)
BsaHI 1756 GGCGTC G[GA]^CG[CT]C
BsgI 1234 CTGCAC GTGCAG(16/14)
BspHI 522 TCATGA T^CATGA
BspLU11I 3242 ACATGT A^CATGT
BssSI 1178 CACGAG CACGAG(-5/-1)
Bst1107I 571 GTATAC GTA^TAC
BstXI 3447 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 571 GTATAC GTA^TAC
Bsu36I 3480 CCTGAGG CC^T[ACGT]AGG
CfoI 3664 GCGC GCG^C
DraII 999 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 994 CCATGG C^C[GA][CT]GG
Eco81I 3480 CCTGAGG CC^T[ACGT]AGG
EcoO109I 999 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 437 GAATTC G^AATTC
EcoT22I 2145 ATGCAT ATGCA^T
FauI 3468 GCGGG CCCGC(4/6)
HapII 3624 CCGG C^CGG
HgaI 1757 GCGTC GACGC(5/10)
HhaI 3664 GCGC GCG^C
Hin1I 1756 GGCGTC G[GA]^CG[CT]C
HinP1I 3664 GCGC G^CGC
HpaII 3624 CCGG C^CGG
KpnI 3766 GGTACC GGTAC^C
MslI 894 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 3624 CCGG C^CGG
NcoI 994 CCATGG C^CATGG
NsiI 2145 ATGCAT ATGCA^T
NspI 3242 ACATGT [GA]CATG^[CT]
PpuMI 999 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 522 TCATGA T^CATGA
SacI 1015 GAGCTC GAGCT^C
SmaI 3623 CCCGGG CCC^GGG
XbaI 1455 TCTAGA T^CTAGA
XcmI 985 CCAAATACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3623 CCCGGG C^CCGGG
XmaI 3623 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Asp700I 1837 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2980 GAAATCATTC
AspI 1262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1611 GACCGAGTC
AvaI 3591 CTCGGG C^[CT]CG[GA]G
3623 CCCGGG
BalI 3416 TGGCCA TGG^CCA
3507 TGGCCA
BanI 1579 GGCACC G^G[CT][GA]CC
3766 GGTACC
BanII 1015 GAGCTC G[GA]GC[CT]^C
2561 GGGCTC
BcgI 1177 GCACGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3799 GCATTTCTTTCG
BclI 816 TGATCA T^GATCA
2420 TGATCA
BcnI 3623 CCCGG CC^[GC]GG
3624 CCGGG
BpmI 1239 CTCCAG CTGGAG(16/14)
979 CTGGAG
BsaAI 236 CACGTA [CT]AC^GT[GA]
2972 CACGTA
BsoBI 3591 CTCGGG C^[CT]CG[GA]G
3623 CCCGGG
Bsp1407I 2548 TGTACA T^GTACA
3239 TGTACA
BspMI 1400 GCAGGT ACCTGC(4/8)
2207 GCAGGT
BsrGI 2548 TGTACA T^GTACA
3239 TGTACA
BstAPI 1203 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3631 GCAGAGGTTGC
BstBI 544 TTCGAA TT^CGAA
3135 TTCGAA
Cac8I 1701 GCACGC GC[ACGT]^[ACGT]GC
3468 GCGGGC
DraIII 809 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1411 CACCCTGTG
EaeI 3416 TGGCCA [CT]^GGCC[GA]
3507 TGGCCA
EarI 1805 CTCTTC CTCTTC(1/4)
3400 CTCTTC
EcoT14I 994 CCATGG C^C[AT][AT]GG
3049 CCAAGG
FbaI 816 TGATCA T^GATCA
2420 TGATCA
Ksp632I 1805 CTCTTC CTCTTC(1/4)
3400 CTCTTC
MfeI 597 CAATTG C^AATTG
3101 CAATTG
MluNI 3416 TGGCCA TGG^CCA
3507 TGGCCA
MscI 3416 TGGCCA TGG^CCA
3507 TGGCCA
MunI 597 CAATTG C^AATTG
3101 CAATTG
NciI 3623 CCCGG CC^[GC]GG
3624 CCGGG
NspV 544 TTCGAA TT^CGAA
3135 TTCGAA
PflFI 1262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1611 GACCGAGTC
SfuI 544 TTCGAA TT^CGAA
3135 TTCGAA
SpeI 141 ACTAGT A^CTAGT
2467 ACTAGT
SspBI 2548 TGTACA T^GTACA
3239 TGTACA
StyI 994 CCATGG C^C[AT][AT]GG
3049 CCAAGG
Tth111I 1262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1611 GACCGAGTC
XmnI 1837 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2980 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbvI BfaI BmyI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI PshBI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII