##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I AvrII BamHI BbrPI BclI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoT22I FbaI FseI HpaI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 920 GGTACC G^GTACC
AccI 1015 GTCTAC GT^[AC][GT]AC
AhdI 340 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2761 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2731 AGCGCT AGC^GCT
Asp718I 920 GGTACC G^GTACC
AspEI 340 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2415 TGCGCA TGC^GCA
BcgI 1544 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3436 GTATCC GTATCC(6/5)
BfaI 2194 CTAG C^TAG
BglII 1697 AGATCT A^GATCT
BlpI 2727 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2727 GCTAAGC GC^T[ACGT]AGC
Bsp1407I 512 TGTACA T^GTACA
BspLU11I 416 ACATGT A^CATGT
BsrGI 512 TGTACA T^GTACA
BstEII 2799 GGTTACC G^GT[ACGT]ACC
BstPI 2799 GGTTACC G^GT[ACGT]ACC
Bsu36I 2761 CCTCAGG CC^T[ACGT]AGG
CelII 2727 GCTAAGC GC^T[ACGT]AGC
DraIII 2946 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2485 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 340 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2731 AGCGCT AGC^GCT
Eco81I 2761 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2799 GGTTACC G^GT[ACGT]ACC
EcoRV 217 GATATC GAT^ATC
FspI 2415 TGCGCA TGC^GCA
HincII 3331 GTCAAC GT[CT]^[GA]AC
HindII 3331 GTCAAC GT[CT]^[GA]AC
HindIII 787 AAGCTT A^AGCTT
KpnI 920 GGTACC GGTAC^C
KspI 2401 CCGCGG CCGC^GG
MaeI 2194 CTAG C^TAG
NaeI 1520 GCCGGC GCC^GGC
NcoI 2745 CCATGG C^CATGG
NgoMIV 1520 GCCGGC G^CCGGC
NspI 416 ACATGT [GA]CATG^[CT]
PshAI 1484 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 506 GAGCTC GAGCT^C
SacII 2401 CCGCGG CCGC^GG
ScaI 365 AGTACT AGT^ACT
SfiI 1742 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 512 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 2402 CGCG CG^CG
2646 CGCG
AflIII 416 ACATGT A^C[GA][CT]GT
2286 ACACGT
ApaI 245 GGGCCC GGGCC^C
1229 GGGCCC
AspI 2215 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3740 GACTCCGTC
BbeI 269 GGCGCC GGCGC^C
1161 GGCGCC
BsaI 2020 GAGACC GGTCTC(1/5)
3553 GAGACC
BspHI 383 TCATGA T^CATGA
815 TCATGA
BspMI 1216 ACCTGC ACCTGC(4/8)
1719 GCAGGT
BsrFI 1464 GCCGGT [GA]^CCGG[CT]
1520 GCCGGC
BstUI 2402 CGCG CG^CG
2646 CGCG
BstYI 459 GGATCT [GA]^GATC[CT]
1697 AGATCT
Cfr10I 1464 GCCGGT [GA]^CCGG[CT]
1520 GCCGGC
EcoRI 625 GAATTC G^AATTC
2856 GAATTC
KasI 269 GGCGCC G^GCGCC
1161 GGCGCC
MflI 459 GGATCT [GA]^GATC[CT]
1697 AGATCT
MseI 1924 TTAA T^TAA
2304 TTAA
MslI 168 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2314 CACCAGTGTG
MvnI 2402 CGCG CG^CG
2646 CGCG
NarI 269 GGCGCC GG^CGCC
1161 GGCGCC
PflFI 2215 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3740 GACTCCGTC
PvuII 1449 CAGCTG CAG^CTG
1459 CAGCTG
RcaI 383 TCATGA T^CATGA
815 TCATGA
SfoI 269 GGCGCC GGC^GCC
1161 GGCGCC
TaqI 2018 TCGA T^CGA
3551 TCGA
Tru9I 1924 TTAA T^TAA
2304 TTAA
Tth111I 2215 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3740 GACTCCGTC
TthHB8I 2018 TCGA T^CGA
3551 TCGA
XcmI 80 CCACGAGCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCACGGAGTACCTGG
XhoII 459 GGATCT [GA]^GATC[CT]
1697 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XmaCI XmaI