##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspI        BamHI       BbeI        BbrPI       
BcgI        BcnI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MfeI        MflI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        Tth111I     XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       1016  GGTACC               G^GTACC
AflII          56  CTTAAG               C^TTAAG
AlwI         1478  GGATC                GGATC(4/5)
Asp700I      1991  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1016  GGTACC               G^GTACC
AviII        2510  TGCGCA               TGC^GCA
AvrII        1628  CCTAGG               C^CTAGG
BalI          976  TGGCCA               TGG^CCA
BanI         1016  GGTACC               G^G[CT][GA]CC
BbvCI        1293  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3038  GGATAC               GTATCC(6/5)
BclI         2384  TGATCA               T^GATCA
BfrI           56  CTTAAG               C^TTAAG
BlnI         1628  CCTAGG               C^CTAGG
BsaBI        2683  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1408  GGTCTC               GGTCTC(1/5)
BsmBI         920  GAGACG               CGTCTC(1/5)
BsmFI        1660  GGGAC                GGGAC(10/14)
BsmI         2513  GCATTC               GAATGC(1/-1)
BspHI          44  TCATGA               T^CATGA
BspMI         420  ACCTGC               ACCTGC(4/8)
Bst1107I      942  GTATAC               GTA^TAC
BstXI         691  CCAGCATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       942  GTATAC               GTA^TAC
CfoI         2511  GCGC                 GCG^C
EaeI          976  TGGCCA               [CT]^GGCC[GA]
EarI         2824  CTCTTC               CTCTTC(1/4)
FbaI         2384  TGATCA               T^GATCA
FspI         2510  TGCGCA               TGC^GCA
HapII         586  CCGG                 C^CGG
HhaI         2511  GCGC                 GCG^C
HinP1I       2511  GCGC                 G^CGC
HpaII         586  CCGG                 C^CGG
KpnI         1016  GGTACC               GGTAC^C
Ksp632I      2824  CTCTTC               CTCTTC(1/4)
MaeII         923  ACGT                 A^CGT
MamI         2683  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         976  TGGCCA               TGG^CCA
MscI          976  TGGCCA               TGG^CCA
MspI          586  CCGG                 C^CGG
NdeI         2878  CATATG               CA^TATG
NspI          661  GCATGC               [GA]CATG^[CT]
PstI          739  CTGCAG               CTGCA^G
RcaI           44  TCATGA               T^CATGA
SacI         1849  GAGCTC               GAGCT^C
SexAI        1012  ACCTGGT              A^CC[AT]GGT
SphI          661  GCATGC               GCATG^C
StuI         2366  AGGCCT               AGG^CCT
XmnI         1991  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         954  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1008  CAGGACCTG           
AocI          818  CCTGAGG              CC^T[ACGT]AGG
             2276  CCTTAGG             
AvaI         1446  CCCGAG               C^[CT]CG[GA]G
             2362  CCCGAG              
AvaII        1010  GGACC                G^G[AT]CC
             1661  GGACC               
BsoBI        1446  CCCGAG               C^[CT]CG[GA]G
             2362  CCCGAG              
Bsp1407I     1191  TGTACA               T^GTACA
             1888  TGTACA              
BsrGI        1191  TGTACA               T^GTACA
             1888  TGTACA              
Bsu36I        818  CCTGAGG              CC^T[ACGT]AGG
             2276  CCTTAGG             
DraII        1009  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1660  GGGACCT             
Eco81I        818  CCTGAGG              CC^T[ACGT]AGG
             2276  CCTTAGG             
EcoO109I     1009  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1660  GGGACCT             
EcoRV         703  GATATC               GAT^ATC
             1098  GATATC              
PflMI         425  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              979  CCAAGGAATGG         
PpuMI        1009  AGGACCT              [GA]G^G[AT]CC[CT]
             1660  GGGACCT             
PvuII         157  CAGCTG               CAG^CTG
             1696  CAGCTG              
ScaI         1076  AGTACT               AGT^ACT
             2913  AGTACT              
SmlI           56  CTTAAG               C^T[CT][GA]AG
             1858  CTCAAG              
SspBI        1191  TGTACA               T^GTACA
             1888  TGTACA              
SspI         2611  AATATT               AAT^ATT
             2843  AATATT              
Van91I        425  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              979  CCAAGGAATGG         
XbaI         2059  TCTAGA               T^CTAGA
             2194  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
ApoI        AspHI       BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HindIII     HinfI       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I