##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspEI       
BbeI        BcgI        BclI        BfrI        BsaBI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI         1107  GACGCC               G[GA]^CG[CT]C
Alw44I       1242  GTGCAC               G^TGCAC
ApaLI        1242  GTGCAC               G^TGCAC
AspI          633  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         575  CCTAGG               C^CTAGG
BamHI        3026  GGATCC               G^GATCC
BbrPI        2173  CACGTG               CAC^GTG
BbvCI        2231  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1652  GTATCC               GTATCC(6/5)
BlnI          575  CCTAGG               C^CTAGG
BsaHI        1107  GACGCC               G[GA]^CG[CT]C
BsaI          939  GAGACC               GGTCTC(1/5)
BsaWI        1476  TCCGGT               [AT]^CCGG[AT]
BsmBI        2711  GAGACG               CGTCTC(1/5)
BspMI         962  GCAGGT               ACCTGC(4/8)
BsrFI        2613  GCCGGT               [GA]^CCGG[CT]
BssSI        2855  CTCGTG               CACGAG(-5/-1)
BstEII        112  GGTGACC              G^GT[ACGT]ACC
BstPI         112  GGTGACC              G^GT[ACGT]ACC
Cfr10I       2613  GCCGGT               [GA]^CCGG[CT]
DraIII       2742  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       112  GGTGACC              G^GT[ACGT]ACC
Hin1I        1107  GACGCC               G[GA]^CG[CT]C
HindIII      2820  AAGCTT               A^AGCTT
NdeI          983  CATATG               CA^TATG
NspI         2151  GCATGC               [GA]CATG^[CT]
PflFI         633  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2173  CACGTG               CAC^GTG
PmlI         2173  CACGTG               CAC^GTG
PvuII        1914  CAGCTG               CAG^CTG
SapI         2237  GAAGAGC              GCTCTTC(1/4)
SmaI         2563  CCCGGG               CCC^GGG
SphI         2151  GCATGC               GCATG^C
Tth111I       633  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2563  CCCGGG               C^CCGGG
XmaI         2563  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          733  GTAGAC               GT^[AC][GT]AC
             1480  GTAGAC              
AcsI          523  AAATTC               [GA]^AATT[CT]
             3178  AAATTT              
ApoI          523  AAATTC               [GA]^AATT[CT]
             3178  AAATTT              
Asp700I       740  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              913  GAACTGCTTC          
BalI          781  TGGCCA               TGG^CCA
             1640  TGGCCA              
BglI          170  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              980  GCCCATATGGC         
BglII        1120  AGATCT               A^GATCT
             2621  AGATCT              
BlpI           17  GCTCAGC              GC^T[ACGT]AGC
             2146  GCTGAGC             
Bpu1102I       17  GCTCAGC              GC^T[ACGT]AGC
             2146  GCTGAGC             
BsmI           43  GCATTC               GAATGC(1/-1)
             2338  GCATTC              
CelII          17  GCTCAGC              GC^T[ACGT]AGC
             2146  GCTGAGC             
DsaI          502  CCATGG               C^C[GA][CT]GG
             1637  CCATGG              
EcoNI         924  CCTAGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3038  CCTATGGAAGG         
MluNI         781  TGGCCA               TGG^CCA
             1640  TGGCCA              
MscI          781  TGGCCA               TGG^CCA
             1640  TGGCCA              
MseI         1383  TTAA                 T^TAA
             2385  TTAA                
MspA1I       1914  CAGCTG               C[AC]G^C[GT]G
             2953  CAGCGG              
NcoI          502  CCATGG               C^CATGG
             1637  CCATGG              
SacI           15  GAGCTC               GAGCT^C
             1341  GAGCTC              
TfiI         1280  GATTC                G^A[AT]TC
             3134  GATTC               
Tru9I        1383  TTAA                 T^TAA
             2385  TTAA                
XcmI         1942  CCAAATAGCATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2172  CCACGTGGGACCTGG     
XmnI          740  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              913  GAACTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
AviII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII