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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspHI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII EagI EarI
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI
SgrAI SmaI SpeI SphI SspBI
StuI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1913 GTAGAC GT^[AC][GT]AC
AflIII 1695 ACATGT A^C[GA][CT]GT
AhdI 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2000 CCTAAGG CC^T[ACGT]AGG
AspEI 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1283 GGTCC G^G[AT]CC
BalI 1442 TGGCCA TGG^CCA
BamHI 1675 GGATCC G^GATCC
BanII 2802 GAGCCC G[GA]GC[CT]^C
BclI 2783 TGATCA T^GATCA
BsmAI 1379 GAGAC GTCTC(1/5)
BsmI 166 GCATTC GAATGC(1/-1)
BspLU11I 1695 ACATGT A^CATGT
BssKI 1351 CCAGG ^CC[ACGT]GG
BstBI 1710 TTCGAA TT^CGAA
BstNI 1351 CCAGG CC^[AT]GG
Bsu36I 2000 CCTAAGG CC^T[ACGT]AGG
Cfr13I 1283 GGTCC G^G[ACGT]CC
DraIII 2766 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1275 GACTATTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1442 TGGCCA [CT]^GGCC[GA]
Eam1105I 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2000 CCTAAGG CC^T[ACGT]AGG
EcoNI 3050 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 1351 CCAGG ^CC[AT]GG
EcoT22I 2613 ATGCAT ATGCA^T
FauI 1735 GCGGG CCCGC(4/6)
FbaI 2783 TGATCA T^GATCA
MfeI 768 CAATTG C^AATTG
MluNI 1442 TGGCCA TGG^CCA
MscI 1442 TGGCCA TGG^CCA
MspA1I 192 CAGCTG C[AC]G^C[GT]G
MunI 768 CAATTG C^AATTG
MvaI 1351 CCAGG CC^[AT]GG
NsiI 2613 ATGCAT ATGCA^T
NspV 1710 TTCGAA TT^CGAA
PflMI 2765 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 192 CAGCTG CAG^CTG
Sau96I 1283 GGTCC G^G[ACGT]CC
SbfI 1586 CCTGCAGG CCTGCA^GG
ScaI 206 AGTACT AGT^ACT
ScrFI 1351 CCAGG CC^[ACGT]GG
SfaNI 535 GATGC GCATC(5/9)
SfuI 1710 TTCGAA TT^CGAA
SmlI 2088 CTCAAG C^T[CT][GA]AG
Sse8387I 1586 CCTGCAGG CCTGCA^GG
SwaI 267 ATTTAAAT ATTT^AAAT
Van91I 2765 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 909 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1773 CAGAGGCTG
AseI 110 ATTAAT AT^TAAT
1313 ATTAAT
AsnI 110 ATTAAT AT^TAAT
1313 ATTAAT
BbsI 1567 GAAGAC GAAGAC(2/6)
2910 GAAGAC
BciVI 761 GGATAC GTATCC(6/5)
128 GTATCC
BglII 291 AGATCT A^GATCT
1266 AGATCT
BmyI 2802 GAGCCC G[AGT]GC[ACT]^C
3059 GGGCAC
BpmI 295 CTGGAG CTGGAG(16/14)
2305 CTGGAG
BpuAI 1567 GAAGAC GAAGAC(2/6)
2910 GAAGAC
BsaAI 2571 TACGTA [CT]AC^GT[GA]
2930 TACGTA
BsmFI 2241 GGGAC GGGAC(10/14)
2017 GTCCC
Bsp1286I 2802 GAGCCC G[AGT]GC[ACT]^C
3059 GGGCAC
BspMI 930 ACCTGC ACCTGC(4/8)
1585 ACCTGC
EcoRV 56 GATATC GAT^ATC
503 GATATC
EcoT14I 405 CCATGG C^C[AT][AT]GG
893 CCATGG
HaeIII 1349 GGCC GG^CC
1443 GGCC
NcoI 405 CCATGG C^CATGG
893 CCATGG
PleI 50 GACTC GAGTC(4/5)
2951 GACTC
PshBI 110 ATTAAT AT^TAAT
1313 ATTAAT
PstI 932 CTGCAG CTGCA^G
1587 CTGCAG
SnaBI 2571 TACGTA TAC^GTA
2930 TACGTA
SspI 1316 AATATT AAT^ATT
3087 AATATT
StyI 405 CCATGG C^C[AT][AT]GG
893 CCATGG
XcmI 470 CCATCTGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1752 CCACTCACTATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BsaJI
BseRI BsiYI BslI BsrDI BsrI
BstF5I BstYI Cac8I DdeI DpnII
DraI DsaI Fnu4HI FokI HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI