################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII EagI EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SgrAI SmaI SpeI SphI SspBI StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1913 GTAGAC GT^[AC][GT]AC AflIII 1695 ACATGT A^C[GA][CT]GT AhdI 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2000 CCTAAGG CC^T[ACGT]AGG AspEI 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1283 GGTCC G^G[AT]CC BalI 1442 TGGCCA TGG^CCA BamHI 1675 GGATCC G^GATCC BanII 2802 GAGCCC G[GA]GC[CT]^C BclI 2783 TGATCA T^GATCA BsmAI 1379 GAGAC GTCTC(1/5) BsmI 166 GCATTC GAATGC(1/-1) BspLU11I 1695 ACATGT A^CATGT BssKI 1351 CCAGG ^CC[ACGT]GG BstBI 1710 TTCGAA TT^CGAA BstNI 1351 CCAGG CC^[AT]GG Bsu36I 2000 CCTAAGG CC^T[ACGT]AGG Cfr13I 1283 GGTCC G^G[ACGT]CC DraIII 2766 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1275 GACTATTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1442 TGGCCA [CT]^GGCC[GA] Eam1105I 1101 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2000 CCTAAGG CC^T[ACGT]AGG EcoNI 3050 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 1351 CCAGG ^CC[AT]GG EcoT22I 2613 ATGCAT ATGCA^T FauI 1735 GCGGG CCCGC(4/6) FbaI 2783 TGATCA T^GATCA MfeI 768 CAATTG C^AATTG MluNI 1442 TGGCCA TGG^CCA MscI 1442 TGGCCA TGG^CCA MspA1I 192 CAGCTG C[AC]G^C[GT]G MunI 768 CAATTG C^AATTG MvaI 1351 CCAGG CC^[AT]GG NsiI 2613 ATGCAT ATGCA^T NspV 1710 TTCGAA TT^CGAA PflMI 2765 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 192 CAGCTG CAG^CTG Sau96I 1283 GGTCC G^G[ACGT]CC SbfI 1586 CCTGCAGG CCTGCA^GG ScaI 206 AGTACT AGT^ACT ScrFI 1351 CCAGG CC^[ACGT]GG SfaNI 535 GATGC GCATC(5/9) SfuI 1710 TTCGAA TT^CGAA SmlI 2088 CTCAAG C^T[CT][GA]AG Sse8387I 1586 CCTGCAGG CCTGCA^GG SwaI 267 ATTTAAAT ATTT^AAAT Van91I 2765 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 909 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1773 CAGAGGCTG AseI 110 ATTAAT AT^TAAT 1313 ATTAAT AsnI 110 ATTAAT AT^TAAT 1313 ATTAAT BbsI 1567 GAAGAC GAAGAC(2/6) 2910 GAAGAC BciVI 761 GGATAC GTATCC(6/5) 128 GTATCC BglII 291 AGATCT A^GATCT 1266 AGATCT BmyI 2802 GAGCCC G[AGT]GC[ACT]^C 3059 GGGCAC BpmI 295 CTGGAG CTGGAG(16/14) 2305 CTGGAG BpuAI 1567 GAAGAC GAAGAC(2/6) 2910 GAAGAC BsaAI 2571 TACGTA [CT]AC^GT[GA] 2930 TACGTA BsmFI 2241 GGGAC GGGAC(10/14) 2017 GTCCC Bsp1286I 2802 GAGCCC G[AGT]GC[ACT]^C 3059 GGGCAC BspMI 930 ACCTGC ACCTGC(4/8) 1585 ACCTGC EcoRV 56 GATATC GAT^ATC 503 GATATC EcoT14I 405 CCATGG C^C[AT][AT]GG 893 CCATGG HaeIII 1349 GGCC GG^CC 1443 GGCC NcoI 405 CCATGG C^CATGG 893 CCATGG PleI 50 GACTC GAGTC(4/5) 2951 GACTC PshBI 110 ATTAAT AT^TAAT 1313 ATTAAT PstI 932 CTGCAG CTGCA^G 1587 CTGCAG SnaBI 2571 TACGTA TAC^GTA 2930 TACGTA SspI 1316 AATATT AAT^ATT 3087 AATATT StyI 405 CCATGG C^C[AT][AT]GG 893 CCATGG XcmI 470 CCATCTGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1752 CCACTCACTATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BsaJI BseRI BsiYI BslI BsrDI BsrI BstF5I BstYI Cac8I DdeI DpnII DraI DsaI Fnu4HI FokI HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI