##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
AflIII      AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BseRI       BsiEI       
BsmI        BspDI       BspHI       BspLU11I    BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
MamI        MluI        MluNI       MscI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII        2278  CGCG                 CG^CG
AccIII       1167  TCCGGA               T^CCGGA
AgeI         1415  ACCGGT               A^CCGGT
Alw44I        389  GTGCAC               G^TGCAC
AlwNI        2804  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1476  GGGCCC               GGGCC^C
ApaLI         389  GTGCAC               G^TGCAC
BfaI          682  CTAG                 C^TAG
BlpI         3039  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3039  GCTGAGC              GC^T[ACGT]AGC
BsaI         2195  GGTCTC               GGTCTC(1/5)
BseAI        1167  TCCGGA               T^CCGGA
BsiWI         194  CGTACG               C^GTACG
BsmBI        3005  CGTCTC               CGTCTC(1/5)
Bsp1407I      830  TGTACA               T^GTACA
BspEI        1167  TCCGGA               T^CCGGA
BsrBI        2262  GAGCGG               CCGCTC(-3/-3)
BsrGI         830  TGTACA               T^GTACA
BstUI        2278  CGCG                 CG^CG
CelII        3039  GCTGAGC              GC^T[ACGT]AGC
CfoI         2440  GCGC                 GCG^C
DraII        1682  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII       2287  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          410  TGGCCG               [CT]^GGCC[GA]
EarI         2534  GAAGAG               CTCTTC(1/4)
EcoO109I     1682  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         329  GATATC               GAT^ATC
HaeII        2439  GGCGCT               [GA]GCGC^[CT]
HgaI         3036  GACGC                GACGC(5/10)
HhaI         2440  GCGC                 GCG^C
HinP1I       2440  GCGC                 G^CGC
Ksp632I      2534  GAAGAG               CTCTTC(1/4)
KspI         2277  CCGCGG               CCGC^GG
MaeI          682  CTAG                 C^TAG
MfeI         1265  CAATTG               C^AATTG
MroI         1167  TCCGGA               T^CCGGA
MunI         1265  CAATTG               C^AATTG
MvnI         2278  CGCG                 CG^CG
PinAI        1415  ACCGGT               A^CCGGT
SacII        2277  CCGCGG               CCGC^GG
SbfI         2946  CCTGCAGG             CCTGCA^GG
ScaI         2755  AGTACT               AGT^ACT
SphI         2848  GCATGC               GCATG^C
Sse8387I     2946  CCTGCAGG             CCTGCA^GG
SspBI         830  TGTACA               T^GTACA
SspI          972  AATATT               AAT^ATT
StuI          138  AGGCCT               AGG^CCT
SwaI         3266  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I       2076  GGTACC               G^GTACC
             2505  GGTACC              
Asp700I        77  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2363  GAATTGGTTC          
Asp718I      2076  GGTACC               G^GTACC
             2505  GGTACC              
AspI         1339  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1564  GACTTTGTC           
AvaII        1950  GGACC                G^G[AT]CC
             2981  GGACC               
BamHI         762  GGATCC               G^GATCC
              791  GGATCC              
BciVI        2416  GGATAC               GTATCC(6/5)
             1518  GTATCC              
BclI           62  TGATCA               T^GATCA
             2639  TGATCA              
BsaWI        1167  TCCGGA               [AT]^CCGG[AT]
             1415  ACCGGT              
DraI         1774  TTTAAA               TTT^AAA
             3267  TTTAAA              
EcoRI        2086  GAATTC               G^AATTC
             2624  GAATTC              
EcoT22I       856  ATGCAT               ATGCA^T
             1446  ATGCAT              
FauI         1310  CCCGC                CCCGC(4/6)
             2276  CCCGC               
FbaI           62  TGATCA               T^GATCA
             2639  TGATCA              
KpnI         2076  GGTACC               GGTAC^C
             2505  GGTACC              
NaeI          520  GCCGGC               GCC^GGC
             2383  GCCGGC              
NgoMIV        520  GCCGGC               G^CCGGC
             2383  GCCGGC              
NsiI          856  ATGCAT               ATGCA^T
             1446  ATGCAT              
NspI         1444  ACATGC               [GA]CATG^[CT]
             2848  GCATGC              
PflFI        1339  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1564  GACTTTGTC           
Tth111I      1339  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1564  GACTTTGTC           
XmnI           77  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2363  GAATTGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        BanI        BanII       
BbvI        BcgI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EcoNI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HindIII     HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI