################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AhdI AocI Aor51HI AscI AseI AsnI AspEI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BseRI BsiEI BsmI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI Hin1I HincII HindII HpaI KasI MamI MluI MluNI MscI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 2278 CGCG CG^CG AccIII 1167 TCCGGA T^CCGGA AgeI 1415 ACCGGT A^CCGGT Alw44I 389 GTGCAC G^TGCAC AlwNI 2804 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1476 GGGCCC GGGCC^C ApaLI 389 GTGCAC G^TGCAC BfaI 682 CTAG C^TAG BlpI 3039 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3039 GCTGAGC GC^T[ACGT]AGC BsaI 2195 GGTCTC GGTCTC(1/5) BseAI 1167 TCCGGA T^CCGGA BsiWI 194 CGTACG C^GTACG BsmBI 3005 CGTCTC CGTCTC(1/5) Bsp1407I 830 TGTACA T^GTACA BspEI 1167 TCCGGA T^CCGGA BsrBI 2262 GAGCGG CCGCTC(-3/-3) BsrGI 830 TGTACA T^GTACA BstUI 2278 CGCG CG^CG CelII 3039 GCTGAGC GC^T[ACGT]AGC CfoI 2440 GCGC GCG^C DraII 1682 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 2287 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 410 TGGCCG [CT]^GGCC[GA] EarI 2534 GAAGAG CTCTTC(1/4) EcoO109I 1682 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 329 GATATC GAT^ATC HaeII 2439 GGCGCT [GA]GCGC^[CT] HgaI 3036 GACGC GACGC(5/10) HhaI 2440 GCGC GCG^C HinP1I 2440 GCGC G^CGC Ksp632I 2534 GAAGAG CTCTTC(1/4) KspI 2277 CCGCGG CCGC^GG MaeI 682 CTAG C^TAG MfeI 1265 CAATTG C^AATTG MroI 1167 TCCGGA T^CCGGA MunI 1265 CAATTG C^AATTG MvnI 2278 CGCG CG^CG PinAI 1415 ACCGGT A^CCGGT SacII 2277 CCGCGG CCGC^GG SbfI 2946 CCTGCAGG CCTGCA^GG ScaI 2755 AGTACT AGT^ACT SphI 2848 GCATGC GCATG^C Sse8387I 2946 CCTGCAGG CCTGCA^GG SspBI 830 TGTACA T^GTACA SspI 972 AATATT AAT^ATT StuI 138 AGGCCT AGG^CCT SwaI 3266 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 2076 GGTACC G^GTACC 2505 GGTACC Asp700I 77 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2363 GAATTGGTTC Asp718I 2076 GGTACC G^GTACC 2505 GGTACC AspI 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1564 GACTTTGTC AvaII 1950 GGACC G^G[AT]CC 2981 GGACC BamHI 762 GGATCC G^GATCC 791 GGATCC BciVI 2416 GGATAC GTATCC(6/5) 1518 GTATCC BclI 62 TGATCA T^GATCA 2639 TGATCA BsaWI 1167 TCCGGA [AT]^CCGG[AT] 1415 ACCGGT DraI 1774 TTTAAA TTT^AAA 3267 TTTAAA EcoRI 2086 GAATTC G^AATTC 2624 GAATTC EcoT22I 856 ATGCAT ATGCA^T 1446 ATGCAT FauI 1310 CCCGC CCCGC(4/6) 2276 CCCGC FbaI 62 TGATCA T^GATCA 2639 TGATCA KpnI 2076 GGTACC GGTAC^C 2505 GGTACC NaeI 520 GCCGGC GCC^GGC 2383 GCCGGC NgoMIV 520 GCCGGC G^CCGGC 2383 GCCGGC NsiI 856 ATGCAT ATGCA^T 1446 ATGCAT NspI 1444 ACATGC [GA]CATG^[CT] 2848 GCATGC PflFI 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1564 GACTTTGTC Tth111I 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1564 GACTTTGTC XmnI 77 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2363 GAATTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI BanI BanII BbvI BcgI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DsaI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI