################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI KpnI MamI MfeI MluI MroI MunI NdeI NheI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AatII 1175 GACGTC GACGT^C AcsI 971 AAATTC [GA]^AATT[CT] ApaI 1650 GGGCCC GGGCC^C ApoI 971 AAATTC [GA]^AATT[CT] Asp700I 2906 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 76 TGCGCA TGC^GCA BamHI 2934 GGATCC G^GATCC BcgI 2894 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiEI 3360 CGGCCG CG[GA][CT]^CG BsmI 1689 GCATTC GAATGC(1/-1) BssSI 2295 CACGAG CACGAG(-5/-1) DrdI 2043 GACAACTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3360 CGGCCG C^GGCCG EarI 2312 GAAGAG CTCTTC(1/4) EclXI 3360 CGGCCG C^GGCCG Eco52I 3360 CGGCCG C^GGCCG FspI 76 TGCGCA TGC^GCA HincII 2417 GTTAAC GT[CT]^[GA]AC HindII 2417 GTTAAC GT[CT]^[GA]AC HpaI 2417 GTTAAC GTT^AAC Ksp632I 2312 GAAGAG CTCTTC(1/4) KspI 3018 CCGCGG CCGC^GG NotI 3359 GCGGCCGC GC^GGCCGC NruI 92 TCGCGA TCG^CGA NspI 546 ACATGC [GA]CATG^[CT] SacII 3018 CCGCGG CCGC^GG SexAI 524 ACCAGGT A^CC[AT]GGT XmnI 2906 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 529 GTAC GT^AC 568 GTAC AocI 641 CCTGAGG CC^T[ACGT]AGG 2278 CCTGAGG BalI 668 TGGCCA TGG^CCA 3321 TGGCCA BbeI 1511 GGCGCC GGCGC^C 1669 GGCGCC BsgI 1538 CTGCAC GTGCAG(16/14) 1829 CTGCAC BsmBI 688 CGTCTC CGTCTC(1/5) 1343 CGTCTC BspHI 296 TCATGA T^CATGA 1022 TCATGA BsrBI 1952 GAGCGG CCGCTC(-3/-3) 2969 GAGCGG BsrDI 820 CATTGC GCAATG(2/0) 290 GCAATG BsrI 104 ACTGG ACTGG(1/-1) 2744 CCAGT BstAPI 369 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1540 GCACCTTCTGC Bsu36I 641 CCTGAGG CC^T[ACGT]AGG 2278 CCTGAGG Eco81I 641 CCTGAGG CC^T[ACGT]AGG 2278 CCTGAGG HindIII 2184 AAGCTT A^AGCTT 2909 AAGCTT KasI 1511 GGCGCC G^GCGCC 1669 GGCGCC MaeII 1176 ACGT A^CGT 1974 ACGT MluNI 668 TGGCCA TGG^CCA 3321 TGGCCA MscI 668 TGGCCA TGG^CCA 3321 TGGCCA NarI 1511 GGCGCC GG^CGCC 1669 GGCGCC PflMI 914 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2137 CCAGATCCTGG RcaI 296 TCATGA T^CATGA 1022 TCATGA RsaI 529 GTAC GT^AC 568 GTAC SacI 899 GAGCTC GAGCT^C 2622 GAGCTC SbfI 1984 CCTGCAGG CCTGCA^GG 2269 CCTGCAGG SfoI 1511 GGCGCC GGC^GCC 1669 GGCGCC SmaI 2854 CCCGGG CCC^GGG 3132 CCCGGG Sse8387I 1984 CCTGCAGG CCTGCA^GG 2269 CCTGCAGG StuI 2709 AGGCCT AGG^CCT 2898 AGGCCT TaqI 656 TCGA T^CGA 1708 TCGA TthHB8I 656 TCGA T^CGA 1708 TCGA Van91I 914 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2137 CCAGATCCTGG XcmI 1210 CCATGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3210 CCAGCCGGCTGCTGG XmaCI 2854 CCCGGG C^CCGGG 3132 CCCGGG XmaI 2854 CCCGGG C^CCGGG 3132 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII