##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        ScaI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AatII        1175  GACGTC               GACGT^C
AcsI          971  AAATTC               [GA]^AATT[CT]
ApaI         1650  GGGCCC               GGGCC^C
ApoI          971  AAATTC               [GA]^AATT[CT]
Asp700I      2906  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          76  TGCGCA               TGC^GCA
BamHI        2934  GGATCC               G^GATCC
BcgI         2894  GCAGAGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI        3360  CGGCCG               CG[GA][CT]^CG
BsmI         1689  GCATTC               GAATGC(1/-1)
BssSI        2295  CACGAG               CACGAG(-5/-1)
DrdI         2043  GACAACTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3360  CGGCCG               C^GGCCG
EarI         2312  GAAGAG               CTCTTC(1/4)
EclXI        3360  CGGCCG               C^GGCCG
Eco52I       3360  CGGCCG               C^GGCCG
FspI           76  TGCGCA               TGC^GCA
HincII       2417  GTTAAC               GT[CT]^[GA]AC
HindII       2417  GTTAAC               GT[CT]^[GA]AC
HpaI         2417  GTTAAC               GTT^AAC
Ksp632I      2312  GAAGAG               CTCTTC(1/4)
KspI         3018  CCGCGG               CCGC^GG
NotI         3359  GCGGCCGC             GC^GGCCGC
NruI           92  TCGCGA               TCG^CGA
NspI          546  ACATGC               [GA]CATG^[CT]
SacII        3018  CCGCGG               CCGC^GG
SexAI         524  ACCAGGT              A^CC[AT]GGT
XmnI         2906  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          529  GTAC                 GT^AC
              568  GTAC                
AocI          641  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
BalI          668  TGGCCA               TGG^CCA
             3321  TGGCCA              
BbeI         1511  GGCGCC               GGCGC^C
             1669  GGCGCC              
BsgI         1538  CTGCAC               GTGCAG(16/14)
             1829  CTGCAC              
BsmBI         688  CGTCTC               CGTCTC(1/5)
             1343  CGTCTC              
BspHI         296  TCATGA               T^CATGA
             1022  TCATGA              
BsrBI        1952  GAGCGG               CCGCTC(-3/-3)
             2969  GAGCGG              
BsrDI         820  CATTGC               GCAATG(2/0)
              290  GCAATG              
BsrI          104  ACTGG                ACTGG(1/-1)
             2744  CCAGT               
BstAPI        369  GCAACTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1540  GCACCTTCTGC         
Bsu36I        641  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
Eco81I        641  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
HindIII      2184  AAGCTT               A^AGCTT
             2909  AAGCTT              
KasI         1511  GGCGCC               G^GCGCC
             1669  GGCGCC              
MaeII        1176  ACGT                 A^CGT
             1974  ACGT                
MluNI         668  TGGCCA               TGG^CCA
             3321  TGGCCA              
MscI          668  TGGCCA               TGG^CCA
             3321  TGGCCA              
NarI         1511  GGCGCC               GG^CGCC
             1669  GGCGCC              
PflMI         914  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2137  CCAGATCCTGG         
RcaI          296  TCATGA               T^CATGA
             1022  TCATGA              
RsaI          529  GTAC                 GT^AC
              568  GTAC                
SacI          899  GAGCTC               GAGCT^C
             2622  GAGCTC              
SbfI         1984  CCTGCAGG             CCTGCA^GG
             2269  CCTGCAGG            
SfoI         1511  GGCGCC               GGC^GCC
             1669  GGCGCC              
SmaI         2854  CCCGGG               CCC^GGG
             3132  CCCGGG              
Sse8387I     1984  CCTGCAGG             CCTGCA^GG
             2269  CCTGCAGG            
StuI         2709  AGGCCT               AGG^CCT
             2898  AGGCCT              
TaqI          656  TCGA                 T^CGA
             1708  TCGA                
TthHB8I       656  TCGA                 T^CGA
             1708  TCGA                
Van91I        914  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2137  CCAGATCCTGG         
XcmI         1210  CCATGCAGCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3210  CCAGCCGGCTGCTGG     
XmaCI        2854  CCCGGG               C^CCGGG
             3132  CCCGGG              
XmaI         2854  CCCGGG               C^CCGGG
             3132  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII