##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1175 GACGTC GACGT^C
AcsI 971 AAATTC [GA]^AATT[CT]
ApaI 1650 GGGCCC GGGCC^C
ApoI 971 AAATTC [GA]^AATT[CT]
Asp700I 2906 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 76 TGCGCA TGC^GCA
BamHI 2934 GGATCC G^GATCC
BcgI 2894 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 3360 CGGCCG CG[GA][CT]^CG
BsmI 1689 GCATTC GAATGC(1/-1)
BssSI 2295 CACGAG CACGAG(-5/-1)
DrdI 2043 GACAACTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3360 CGGCCG C^GGCCG
EarI 2312 GAAGAG CTCTTC(1/4)
EclXI 3360 CGGCCG C^GGCCG
Eco52I 3360 CGGCCG C^GGCCG
FspI 76 TGCGCA TGC^GCA
HincII 2417 GTTAAC GT[CT]^[GA]AC
HindII 2417 GTTAAC GT[CT]^[GA]AC
HpaI 2417 GTTAAC GTT^AAC
Ksp632I 2312 GAAGAG CTCTTC(1/4)
KspI 3018 CCGCGG CCGC^GG
NotI 3359 GCGGCCGC GC^GGCCGC
NruI 92 TCGCGA TCG^CGA
NspI 546 ACATGC [GA]CATG^[CT]
SacII 3018 CCGCGG CCGC^GG
SexAI 524 ACCAGGT A^CC[AT]GGT
XmnI 2906 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 529 GTAC GT^AC
568 GTAC
AocI 641 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
BalI 668 TGGCCA TGG^CCA
3321 TGGCCA
BbeI 1511 GGCGCC GGCGC^C
1669 GGCGCC
BsgI 1538 CTGCAC GTGCAG(16/14)
1829 CTGCAC
BsmBI 688 CGTCTC CGTCTC(1/5)
1343 CGTCTC
BspHI 296 TCATGA T^CATGA
1022 TCATGA
BsrBI 1952 GAGCGG CCGCTC(-3/-3)
2969 GAGCGG
BsrDI 820 CATTGC GCAATG(2/0)
290 GCAATG
BsrI 104 ACTGG ACTGG(1/-1)
2744 CCAGT
BstAPI 369 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1540 GCACCTTCTGC
Bsu36I 641 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
Eco81I 641 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
HindIII 2184 AAGCTT A^AGCTT
2909 AAGCTT
KasI 1511 GGCGCC G^GCGCC
1669 GGCGCC
MaeII 1176 ACGT A^CGT
1974 ACGT
MluNI 668 TGGCCA TGG^CCA
3321 TGGCCA
MscI 668 TGGCCA TGG^CCA
3321 TGGCCA
NarI 1511 GGCGCC GG^CGCC
1669 GGCGCC
PflMI 914 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2137 CCAGATCCTGG
RcaI 296 TCATGA T^CATGA
1022 TCATGA
RsaI 529 GTAC GT^AC
568 GTAC
SacI 899 GAGCTC GAGCT^C
2622 GAGCTC
SbfI 1984 CCTGCAGG CCTGCA^GG
2269 CCTGCAGG
SfoI 1511 GGCGCC GGC^GCC
1669 GGCGCC
SmaI 2854 CCCGGG CCC^GGG
3132 CCCGGG
Sse8387I 1984 CCTGCAGG CCTGCA^GG
2269 CCTGCAGG
StuI 2709 AGGCCT AGG^CCT
2898 AGGCCT
TaqI 656 TCGA T^CGA
1708 TCGA
TthHB8I 656 TCGA T^CGA
1708 TCGA
Van91I 914 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2137 CCAGATCCTGG
XcmI 1210 CCATGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3210 CCAGCCGGCTGCTGG
XmaCI 2854 CCCGGG C^CCGGG
3132 CCCGGG
XmaI 2854 CCCGGG C^CCGGG
3132 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII