##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AvrII       BbsI        BcgI        
BciVI       BfaI        BfrI        BglII       BlnI        
BpuAI       BsaBI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DrdI        Eam1105I    EcoRI       EcoRV       
EcoT22I     HincII      HindII      HpaI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SbfI        SexAI       SfiI        
SfuI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AgeI         2032  ACCGGT               A^CCGGT
Alw44I       2025  GTGCAC               G^TGCAC
AocI          943  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2194  AGCGCT               AGC^GCT
ApaLI        2025  GTGCAC               G^TGCAC
Asp700I      1736  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1084  TGGCCA               TGG^CCA
BamHI         407  GGATCC               G^GATCC
BbrPI        1404  CACGTG               CAC^GTG
BbvCI         696  GCTGAGG              CCTCAGC(-5/-2)
BclI          474  TGATCA               T^GATCA
BsaAI        1404  CACGTG               [CT]AC^GT[GA]
BsmBI          56  CGTCTC               CGTCTC(1/5)
BsmI         1836  GAATGC               GAATGC(1/-1)
BstAPI       1346  GCACCGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         407  GGATCC               [GA]^GATC[CT]
Bsu36I        943  CCTCAGG              CC^T[ACGT]AGG
DraI          345  TTTAAA               TTT^AAA
DraIII       1347  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          795  CTCTTC               CTCTTC(1/4)
Eco47III     2194  AGCGCT               AGC^GCT
Eco81I        943  CCTCAGG              CC^T[ACGT]AGG
FbaI          474  TGATCA               T^GATCA
FseI         1206  GGCCGGCC             GGCCGG^CC
HindIII       630  AAGCTT               A^AGCTT
Ksp632I       795  CTCTTC               CTCTTC(1/4)
KspI         3038  CCGCGG               CCGC^GG
MflI          407  GGATCC               [GA]^GATC[CT]
MluNI        1084  TGGCCA               TGG^CCA
MscI         1084  TGGCCA               TGG^CCA
NcoI         1787  CCATGG               C^CATGG
PflMI        1087  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2032  ACCGGT               A^CCGGT
PmaCI        1404  CACGTG               CAC^GTG
PmlI         1404  CACGTG               CAC^GTG
SacII        3038  CCGCGG               CCGC^GG
SapI          794  GCTCTTC              GCTCTTC(1/4)
SgrAI        2219  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         1503  CTCAAG               C^T[CT][GA]AG
Van91I       1087  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         407  GGATCC               [GA]^GATC[CT]
XmnI         1736  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          580  GAATTT               [GA]^AATT[CT]
             1739  GAATTT              
AlwNI        2154  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2823  CAGTCGCTG           
ApoI          580  GAATTT               [GA]^AATT[CT]
             1739  GAATTT              
BbeI         2116  GGCGCC               GGCGC^C
             2550  GGCGCC              
BlpI            2  GCTCAGC              GC^T[ACGT]AGC
             2365  GCTGAGC             
Bpu1102I        2  GCTCAGC              GC^T[ACGT]AGC
             2365  GCTGAGC             
BspLU11I      954  ACATGT               A^CATGT
             1327  ACATGT              
BsrBI         279  CCGCTC               CCGCTC(-3/-3)
              149  GAGCGG              
BssHII       1342  GCGCGC               G^CGCGC
             2287  GCGCGC              
BstXI        1123  CCAGCCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1213  CCACGGTCATGG        
CelII           2  GCTCAGC              GC^T[ACGT]AGC
             2365  GCTGAGC             
EagI           89  CGGCCG               C^GGCCG
             1205  CGGCCG              
EclXI          89  CGGCCG               C^GGCCG
             1205  CGGCCG              
Eco52I         89  CGGCCG               C^GGCCG
             1205  CGGCCG              
EcoNI         398  CCTCATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2768  CCTGTGCCAGG         
KasI         2116  GGCGCC               G^GCGCC
             2550  GGCGCC              
NarI         2116  GGCGCC               GG^CGCC
             2550  GGCGCC              
PleI         2560  GACTC                GAGTC(4/5)
              707  GAGTC               
PvuII        1898  CAGCTG               CAG^CTG
             2154  CAGCTG              
ScaI         1369  AGTACT               AGT^ACT
             1594  AGTACT              
SfoI         2116  GGCGCC               GGC^GCC
             2550  GGCGCC              
SphI         1534  GCATGC               GCATG^C
             1649  GCATGC              
StuI         1294  AGGCCT               AGG^CCT
             2882  AGGCCT              
XcmI          736  CCAGGGCTTCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1027  CCAACCCGGACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        ApaI        AspHI       AspI        
AvaI        AvaII       AviII       BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflFI       PpuMI       PshAI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XmaCI       XmaI