################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI AscI AseI AsnI Asp718I AspEI AvrII BbsI BcgI BciVI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eam1105I EcoRI EcoRV EcoT22I HincII HindII HpaI KpnI MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AgeI 2032 ACCGGT A^CCGGT Alw44I 2025 GTGCAC G^TGCAC AocI 943 CCTCAGG CC^T[ACGT]AGG Aor51HI 2194 AGCGCT AGC^GCT ApaLI 2025 GTGCAC G^TGCAC Asp700I 1736 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1084 TGGCCA TGG^CCA BamHI 407 GGATCC G^GATCC BbrPI 1404 CACGTG CAC^GTG BbvCI 696 GCTGAGG CCTCAGC(-5/-2) BclI 474 TGATCA T^GATCA BsaAI 1404 CACGTG [CT]AC^GT[GA] BsmBI 56 CGTCTC CGTCTC(1/5) BsmI 1836 GAATGC GAATGC(1/-1) BstAPI 1346 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 407 GGATCC [GA]^GATC[CT] Bsu36I 943 CCTCAGG CC^T[ACGT]AGG DraI 345 TTTAAA TTT^AAA DraIII 1347 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 795 CTCTTC CTCTTC(1/4) Eco47III 2194 AGCGCT AGC^GCT Eco81I 943 CCTCAGG CC^T[ACGT]AGG FbaI 474 TGATCA T^GATCA FseI 1206 GGCCGGCC GGCCGG^CC HindIII 630 AAGCTT A^AGCTT Ksp632I 795 CTCTTC CTCTTC(1/4) KspI 3038 CCGCGG CCGC^GG MflI 407 GGATCC [GA]^GATC[CT] MluNI 1084 TGGCCA TGG^CCA MscI 1084 TGGCCA TGG^CCA NcoI 1787 CCATGG C^CATGG PflMI 1087 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2032 ACCGGT A^CCGGT PmaCI 1404 CACGTG CAC^GTG PmlI 1404 CACGTG CAC^GTG SacII 3038 CCGCGG CCGC^GG SapI 794 GCTCTTC GCTCTTC(1/4) SgrAI 2219 CACCGGCG C[GA]^CCGG[CT]G SmlI 1503 CTCAAG C^T[CT][GA]AG Van91I 1087 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 407 GGATCC [GA]^GATC[CT] XmnI 1736 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 580 GAATTT [GA]^AATT[CT] 1739 GAATTT AlwNI 2154 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2823 CAGTCGCTG ApoI 580 GAATTT [GA]^AATT[CT] 1739 GAATTT BbeI 2116 GGCGCC GGCGC^C 2550 GGCGCC BlpI 2 GCTCAGC GC^T[ACGT]AGC 2365 GCTGAGC Bpu1102I 2 GCTCAGC GC^T[ACGT]AGC 2365 GCTGAGC BspLU11I 954 ACATGT A^CATGT 1327 ACATGT BsrBI 279 CCGCTC CCGCTC(-3/-3) 149 GAGCGG BssHII 1342 GCGCGC G^CGCGC 2287 GCGCGC BstXI 1123 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1213 CCACGGTCATGG CelII 2 GCTCAGC GC^T[ACGT]AGC 2365 GCTGAGC EagI 89 CGGCCG C^GGCCG 1205 CGGCCG EclXI 89 CGGCCG C^GGCCG 1205 CGGCCG Eco52I 89 CGGCCG C^GGCCG 1205 CGGCCG EcoNI 398 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2768 CCTGTGCCAGG KasI 2116 GGCGCC G^GCGCC 2550 GGCGCC NarI 2116 GGCGCC GG^CGCC 2550 GGCGCC PleI 2560 GACTC GAGTC(4/5) 707 GAGTC PvuII 1898 CAGCTG CAG^CTG 2154 CAGCTG ScaI 1369 AGTACT AGT^ACT 1594 AGTACT SfoI 2116 GGCGCC GGC^GCC 2550 GGCGCC SphI 1534 GCATGC GCATG^C 1649 GCATGC StuI 1294 AGGCCT AGG^CCT 2882 AGGCCT XcmI 736 CCAGGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1027 CCAACCCGGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI ApaI AspHI AspI AvaI AvaII AviII BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PpuMI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XmaCI XmaI