##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BbeI BbrPI BcgI
BfrI BglII BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1884 GGTACC G^GTACC
AcsI 1925 GAATTT [GA]^AATT[CT]
AflIII 171 ACATGT A^C[GA][CT]GT
Aor51HI 2538 AGCGCT AGC^GCT
ApoI 1925 GAATTT [GA]^AATT[CT]
Asp718I 1884 GGTACC G^GTACC
AvrII 647 CCTAGG C^CTAGG
BamHI 1024 GGATCC G^GATCC
BbvCI 1349 CCTCAGC CCTCAGC(-5/-2)
BciVI 2617 GTATCC GTATCC(6/5)
BclI 2391 TGATCA T^GATCA
BlnI 647 CCTAGG C^CTAGG
BsmBI 2028 GAGACG CGTCTC(1/5)
BsmI 930 GAATGC GAATGC(1/-1)
BspLU11I 171 ACATGT A^CATGT
BsrFI 691 ACCGGC [GA]^CCGG[CT]
BstXI 2123 CCACATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 691 ACCGGC [GA]^CCGG[CT]
DraIII 698 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3222 CGGCCG C^GGCCG
EclXI 3222 CGGCCG C^GGCCG
Eco47III 2538 AGCGCT AGC^GCT
Eco52I 3222 CGGCCG C^GGCCG
FbaI 2391 TGATCA T^GATCA
HgaI 1088 GACGC GACGC(5/10)
HindIII 162 AAGCTT A^AGCTT
KpnI 1884 GGTACC GGTAC^C
MspA1I 2890 CAGCGG C[AC]G^C[GT]G
NheI 461 GCTAGC G^CTAGC
NotI 3221 GCGGCCGC GC^GGCCGC
PaeR7I 992 CTCGAG C^TCGAG
PflMI 3095 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 3054 CCTGCAGG CCTGCA^GG
ScaI 232 AGTACT AGT^ACT
SfaNI 1136 GCATC GCATC(5/9)
Sse8387I 3054 CCTGCAGG CCTGCA^GG
Van91I 3095 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 992 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 128 CGCG CG^CG
1002 CGCG
AlwNI 2314 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3049 CAGGCCCTG
AocI 66 CCTCAGG CC^T[ACGT]AGG
2353 CCTGAGG
ApaI 1067 GGGCCC GGGCC^C
3015 GGGCCC
AvaI 956 CCCGAG C^[CT]CG[GA]G
992 CTCGAG
BalI 40 TGGCCA TGG^CCA
2489 TGGCCA
BbsI 389 GAAGAC GAAGAC(2/6)
517 GAAGAC
BglI 2886 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3119 GCCTGGCTGGC
BpuAI 389 GAAGAC GAAGAC(2/6)
517 GAAGAC
BsaI 50 GGTCTC GGTCTC(1/5)
1907 GGTCTC
BsiEI 2506 CGGTCG CG[GA][CT]^CG
3222 CGGCCG
BsoBI 956 CCCGAG C^[CT]CG[GA]G
992 CTCGAG
BspMI 1887 ACCTGC ACCTGC(4/8)
2480 ACCTGC
BsrBI 2975 CCGCTC CCGCTC(-3/-3)
2765 GAGCGG
BssSI 2881 CTCGTG CACGAG(-5/-1)
3064 CTCGTG
BstUI 128 CGCG CG^CG
1002 CGCG
Bsu36I 66 CCTCAGG CC^T[ACGT]AGG
2353 CCTGAGG
EarI 980 GAAGAG CTCTTC(1/4)
2445 GAAGAG
Eco81I 66 CCTCAGG CC^T[ACGT]AGG
2353 CCTGAGG
HaeII 2538 AGCGCT [GA]GCGC^[CT]
3091 AGCGCC
HincII 1362 GTCAAC GT[CT]^[GA]AC
2095 GTTGAC
HindII 1362 GTCAAC GT[CT]^[GA]AC
2095 GTTGAC
Ksp632I 980 GAAGAG CTCTTC(1/4)
2445 GAAGAG
MfeI 245 CAATTG C^AATTG
914 CAATTG
MluNI 40 TGGCCA TGG^CCA
2489 TGGCCA
MscI 40 TGGCCA TGG^CCA
2489 TGGCCA
MslI 1210 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2086 CACGTCTGTG
MunI 245 CAATTG C^AATTG
914 CAATTG
MvnI 128 CGCG CG^CG
1002 CGCG
NspI 171 ACATGT [GA]CATG^[CT]
1234 GCATGT
SmlI 992 CTCGAG C^T[CT][GA]AG
1029 CTCAAG
TaqI 54 TCGA T^CGA
993 TCGA
TthHB8I 54 TCGA T^CGA
993 TCGA
XcmI 261 CCAAGTATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
697 CCACTCTGTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII