##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvrII       BalI        BamHI       BanI        BbeI        
BbvCI       BcgI        BclI        BcnI        BglI        
BglII       BlnI        BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstZ17I     
Cac8I       Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRV       FauI        FbaI        FseI        
HaeII       HapII       Hin1I       HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PleI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SphI        Sse8387I    StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AlwNI        2501  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2590  CCTGAGG              CC^T[ACGT]AGG
AviII        1671  TGCGCA               TGC^GCA
BanII        2346  GGGCTC               G[GA]GC[CT]^C
BbrPI        1707  CACGTG               CAC^GTG
BbsI          119  GAAGAC               GAAGAC(2/6)
BbvI         3180  GCTGC                GCAGC(8/12)
BciVI        2366  GTATCC               GTATCC(6/5)
BlpI         2151  GCTTAGC              GC^T[ACGT]AGC
BmyI         2346  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I     2151  GCTTAGC              GC^T[ACGT]AGC
BpuAI         119  GAAGAC               GAAGAC(2/6)
BseRI        1907  CTCCTC               GAGGAG(10/8)
BsmI         1833  GCATTC               GAATGC(1/-1)
Bsp1286I     2346  GGGCTC               G[AGT]GC[ACT]^C
BspMI        2509  GCAGGT               ACCTGC(4/8)
BstBI        3061  TTCGAA               TT^CGAA
BstXI        2557  CCAACACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1222  AGATCC               [GA]^GATC[CT]
Bsu36I       2590  CCTGAGG              CC^T[ACGT]AGG
CelII        2151  GCTTAGC              GC^T[ACGT]AGC
Eco81I       2590  CCTGAGG              CC^T[ACGT]AGG
EcoT14I      2573  CCAAGG               C^C[AT][AT]GG
EcoT22I      2385  ATGCAT               ATGCA^T
Fnu4HI       3180  GCTGC                GC^[ACGT]GC
FspI         1671  TGCGCA               TGC^GCA
HgaI         3183  GCGTC                GACGC(5/10)
HincII       3301  GTTGAC               GT[CT]^[GA]AC
HindII       3301  GTTGAC               GT[CT]^[GA]AC
ItaI         3180  GCTGC                GC^[ACGT]GC
MflI         1222  AGATCC               [GA]^GATC[CT]
NsiI         2385  ATGCAT               ATGCA^T
NspV         3061  TTCGAA               TT^CGAA
PflMI        2732  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1707  CACGTG               CAC^GTG
PmeI         1311  GTTTAAAC             GTTT^AAAC
PmlI         1707  CACGTG               CAC^GTG
PshAI        1883  GACGAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2507  CTGCAG               CTGCA^G
SfuI         3061  TTCGAA               TT^CGAA
SnaBI          74  TACGTA               TAC^GTA
SspI         2886  AATATT               AAT^ATT
StyI         2573  CCAAGG               C^C[AT][AT]GG
TaqI         3062  TCGA                 T^CGA
TseI         3180  GCTGC                G^C[AT]GC
Tsp45I       1881  GTGAC                ^GT[GC]AC
TspRI         934  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I      3062  TCGA                 T^CGA
Van91I       2732  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1222  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI         2004  GTAGAC               GT^[AC][GT]AC
             2226  GTCTAC              
AccII        1892  CGCG                 CG^CG
             2534  CGCG                
AflII        2318  CTTAAG               C^TTAAG
             3450  CTTAAG              
AlwI         1223  GATCC                GGATC(4/5)
             2584  GGATC               
BfrI         2318  CTTAAG               C^TTAAG
             3450  CTTAAG              
BsaAI          74  TACGTA               [CT]AC^GT[GA]
             1707  CACGTG              
BsgI          243  CTGCAC               GTGCAG(16/14)
             2868  GTGCAG              
BsrDI        2768  CATTGC               GCAATG(2/0)
              457  GCAATG              
BstUI        1892  CGCG                 CG^CG
             2534  CGCG                
CfoI         1672  GCGC                 GCG^C
             2533  GCGC                
EcoNI        1142  CCTTTAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3320  CCTGAAGTAGG         
HhaI         1672  GCGC                 GCG^C
             2533  GCGC                
HinP1I       1672  GCGC                 G^CGC
             2533  GCGC                
HindIII       487  AAGCTT               A^AGCTT
             1410  AAGCTT              
MvnI         1892  CGCG                 CG^CG
             2534  CGCG                
SfaNI        3263  GATGC                GCATC(5/9)
             3316  GATGC               
SfcI         1828  CTACAG               C^T[GA][CT]AG
             2507  CTGCAG              
SpeI          560  ACTAGT               A^CTAGT
             1483  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
ApoI        AvaII       BfaI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1407I    BspLU11I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRI       EcoRII      FokI        HaeIII      HinfI       
HphI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MvaI        NdeII       NlaIII      NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SmlI        SspBI       
TfiI        Tru9I       Tsp509I