################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BbeI BbvCI BcgI BclI BcnI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Cac8I Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV FauI FbaI FseI HaeII HapII Hin1I HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI PacI PaeR7I PflFI PinAI PleI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AlwNI 2501 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2590 CCTGAGG CC^T[ACGT]AGG AviII 1671 TGCGCA TGC^GCA BanII 2346 GGGCTC G[GA]GC[CT]^C BbrPI 1707 CACGTG CAC^GTG BbsI 119 GAAGAC GAAGAC(2/6) BbvI 3180 GCTGC GCAGC(8/12) BciVI 2366 GTATCC GTATCC(6/5) BlpI 2151 GCTTAGC GC^T[ACGT]AGC BmyI 2346 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 2151 GCTTAGC GC^T[ACGT]AGC BpuAI 119 GAAGAC GAAGAC(2/6) BseRI 1907 CTCCTC GAGGAG(10/8) BsmI 1833 GCATTC GAATGC(1/-1) Bsp1286I 2346 GGGCTC G[AGT]GC[ACT]^C BspMI 2509 GCAGGT ACCTGC(4/8) BstBI 3061 TTCGAA TT^CGAA BstXI 2557 CCAACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1222 AGATCC [GA]^GATC[CT] Bsu36I 2590 CCTGAGG CC^T[ACGT]AGG CelII 2151 GCTTAGC GC^T[ACGT]AGC Eco81I 2590 CCTGAGG CC^T[ACGT]AGG EcoT14I 2573 CCAAGG C^C[AT][AT]GG EcoT22I 2385 ATGCAT ATGCA^T Fnu4HI 3180 GCTGC GC^[ACGT]GC FspI 1671 TGCGCA TGC^GCA HgaI 3183 GCGTC GACGC(5/10) HincII 3301 GTTGAC GT[CT]^[GA]AC HindII 3301 GTTGAC GT[CT]^[GA]AC ItaI 3180 GCTGC GC^[ACGT]GC MflI 1222 AGATCC [GA]^GATC[CT] NsiI 2385 ATGCAT ATGCA^T NspV 3061 TTCGAA TT^CGAA PflMI 2732 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1707 CACGTG CAC^GTG PmeI 1311 GTTTAAAC GTTT^AAAC PmlI 1707 CACGTG CAC^GTG PshAI 1883 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2507 CTGCAG CTGCA^G SfuI 3061 TTCGAA TT^CGAA SnaBI 74 TACGTA TAC^GTA SspI 2886 AATATT AAT^ATT StyI 2573 CCAAGG C^C[AT][AT]GG TaqI 3062 TCGA T^CGA TseI 3180 GCTGC G^C[AT]GC Tsp45I 1881 GTGAC ^GT[GC]AC TspRI 934 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 3062 TCGA T^CGA Van91I 2732 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1222 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2004 GTAGAC GT^[AC][GT]AC 2226 GTCTAC AccII 1892 CGCG CG^CG 2534 CGCG AflII 2318 CTTAAG C^TTAAG 3450 CTTAAG AlwI 1223 GATCC GGATC(4/5) 2584 GGATC BfrI 2318 CTTAAG C^TTAAG 3450 CTTAAG BsaAI 74 TACGTA [CT]AC^GT[GA] 1707 CACGTG BsgI 243 CTGCAC GTGCAG(16/14) 2868 GTGCAG BsrDI 2768 CATTGC GCAATG(2/0) 457 GCAATG BstUI 1892 CGCG CG^CG 2534 CGCG CfoI 1672 GCGC GCG^C 2533 GCGC EcoNI 1142 CCTTTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3320 CCTGAAGTAGG HhaI 1672 GCGC GCG^C 2533 GCGC HinP1I 1672 GCGC G^CGC 2533 GCGC HindIII 487 AAGCTT A^AGCTT 1410 AAGCTT MvnI 1892 CGCG CG^CG 2534 CGCG SfaNI 3263 GATGC GCATC(5/9) 3316 GATGC SfcI 1828 CTACAG C^T[GA][CT]AG 2507 CTGCAG SpeI 560 ACTAGT A^CTAGT 1483 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI AvaII BfaI BpmI BsaJI BsiYI BslI BsmAI Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII FokI HaeIII HinfI HphI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NspI RsaI Sau3AI Sau96I ScrFI SmlI SspBI TfiI Tru9I Tsp509I