##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BcgI BfrI BglI
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BspEI BspHI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CfoI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI StuI StyI SwaI Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 2433 ACATGT A^C[GA][CT]GT
Alw44I 574 GTGCAC G^TGCAC
ApaLI 574 GTGCAC G^TGCAC
AspHI 574 GTGCAC G[AT]GC[AT]^C
AvaI 2045 CTCGGG C^[CT]CG[GA]G
BanII 382 GAGCCC G[GA]GC[CT]^C
BbrPI 2023 CACGTG CAC^GTG
BbvCI 1920 GCTGAGG CCTCAGC(-5/-2)
BbvI 2 GCTGC GCAGC(8/12)
BciVI 132 GTATCC GTATCC(6/5)
BclI 2762 TGATCA T^GATCA
BlpI 271 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 271 GCTCAGC GC^T[ACGT]AGC
BsaAI 2023 CACGTG [CT]AC^GT[GA]
BseRI 247 GAGGAG GAGGAG(10/8)
BsiHKAI 574 GTGCAC G[AT]GC[AT]^C
BsmBI 2148 CGTCTC CGTCTC(1/5)
BsmI 256 GAATGC GAATGC(1/-1)
BsoBI 2045 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2169 TGTACA T^GTACA
BspDI 1169 ATCGAT AT^CGAT
BspLU11I 2433 ACATGT A^CATGT
BsrGI 2169 TGTACA T^GTACA
Bst1107I 3103 GTATAC GTA^TAC
BstXI 1905 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3103 GTATAC GTA^TAC
Cac8I 749 GCATGC GC[ACGT]^[ACGT]GC
CelII 271 GCTCAGC GC^T[ACGT]AGC
ClaI 1169 ATCGAT AT^CGAT
DraII 87 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 87 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 2762 TGATCA T^GATCA
Fnu4HI 2 GCTGC GC^[ACGT]GC
HincII 767 GTCAAC GT[CT]^[GA]AC
HindII 767 GTCAAC GT[CT]^[GA]AC
ItaI 2 GCTGC GC^[ACGT]GC
MaeII 2024 ACGT A^CGT
MwoI 2477 GCTAGGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI 2023 CACGTG CAC^GTG
PmlI 2023 CACGTG CAC^GTG
PpuMI 87 GGGACCC [GA]G^G[AT]CC[CT]
PstI 53 CTGCAG CTGCA^G
SbfI 52 CCTGCAGG CCTGCA^GG
SexAI 317 ACCTGGT A^CC[AT]GGT
SphI 749 GCATGC GCATG^C
Sse8387I 52 CCTGCAGG CCTGCA^GG
SspBI 2169 TGTACA T^GTACA
TseI 2 GCTGC G^C[AT]GC
XbaI 2738 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2725 GTAGAC GT^[AC][GT]AC
3103 GTATAC
AciI 551 CCGC CCGC(-3/-1)
1881 GCGG
AgeI 1001 ACCGGT A^CCGGT
1421 ACCGGT
Asp700I 711 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
879 GAAACCCTTC
AspI 352 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2143 GACTCCGTC
BbsI 322 GTCTTC GAAGAC(2/6)
1089 GTCTTC
BcnI 85 CCGGG CC^[GC]GG
1875 CCGGG
BglII 2615 AGATCT A^GATCT
2715 AGATCT
BmyI 382 GAGCCC G[AGT]GC[ACT]^C
574 GTGCAC
BpuAI 322 GTCTTC GAAGAC(2/6)
1089 GTCTTC
BsaI 66 GAGACC GGTCTC(1/5)
1953 GAGACC
BsaWI 1001 ACCGGT [AT]^CCGG[AT]
1421 ACCGGT
BsgI 1439 CTGCAC GTGCAG(16/14)
2116 CTGCAC
BsmFI 87 GGGAC GGGAC(10/14)
1425 GTCCC
Bsp1286I 382 GAGCCC G[AGT]GC[ACT]^C
574 GTGCAC
BspMI 51 ACCTGC ACCTGC(4/8)
55 GCAGGT
BsrFI 1001 ACCGGT [GA]^CCGG[CT]
1421 ACCGGT
BstYI 2615 AGATCT [GA]^GATC[CT]
2715 AGATCT
Cfr10I 1001 ACCGGT [GA]^CCGG[CT]
1421 ACCGGT
MflI 2615 AGATCT [GA]^GATC[CT]
2715 AGATCT
MslI 968 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2182 CACAAGTGTG
NciI 85 CCGGG CC^[GC]GG
1875 CCGGG
NspI 749 GCATGC [GA]CATG^[CT]
2433 ACATGT
PflFI 352 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2143 GACTCCGTC
PinAI 1001 ACCGGT A^CCGGT
1421 ACCGGT
PleI 2143 GACTC GAGTC(4/5)
1339 GAGTC
SfaNI 155 GATGC GCATC(5/9)
2891 GATGC
SfcI 53 CTGCAG C^T[GA][CT]AG
2846 CTACAG
SmlI 139 CTCAAG C^T[CT][GA]AG
2495 CTTGAG
TaqI 1170 TCGA T^CGA
1951 TCGA
Tth111I 352 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2143 GACTCCGTC
TthHB8I 1170 TCGA T^CGA
1951 TCGA
XhoII 2615 AGATCT [GA]^GATC[CT]
2715 AGATCT
XmnI 711 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
879 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BalI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRI EcoRII
FokI HaeIII HapII HindIII HinfI
HpaII HphI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MspI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SspI TfiI Tru9I Tsp45I Tsp509I
TspRI