##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
AspEI AspHI AspI AviII BalI
BamHI BbrPI BciVI BlpI Bpu1102I
BsaBI BsiHKAI BsiWI BsmBI BsmI
BspHI BspMI BstAPI BstBI BstEII
BstPI Bsu36I CelII CpoI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HindIII
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfuI SgrAI
SpeI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 2115 GGCGCC G[GA]^CG[CT]C
AflII 2416 CTTAAG C^TTAAG
AflIII 2908 ACATGT A^C[GA][CT]GT
AlwNI 495 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 146 GGGCCC GGGCC^C
AseI 2494 ATTAAT AT^TAAT
AsnI 2494 ATTAAT AT^TAAT
AvaII 228 GGACC G^G[AT]CC
AvrII 3177 CCTAGG C^CTAGG
BbeI 2115 GGCGCC GGCGC^C
BbsI 1 GTCTTC GAAGAC(2/6)
BbvCI 2086 CCTCAGC CCTCAGC(-5/-2)
BcgI 2075 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2416 CTTAAG C^TTAAG
BglI 148 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2447 AGATCT A^GATCT
BlnI 3177 CCTAGG C^CTAGG
BpuAI 1 GTCTTC GAAGAC(2/6)
BsaAI 2553 TACGTA [CT]AC^GT[GA]
BsaHI 2115 GGCGCC G[GA]^CG[CT]C
BspDI 1536 ATCGAT AT^CGAT
BspLU11I 2908 ACATGT A^CATGT
BsrDI 2026 GCAATG GCAATG(2/0)
BsrFI 318 GCCGGT [GA]^CCGG[CT]
BssSI 2203 CTCGTG CACGAG(-5/-1)
BstYI 2447 AGATCT [GA]^GATC[CT]
Cfr10I 318 GCCGGT [GA]^CCGG[CT]
ClaI 1536 ATCGAT AT^CGAT
DraIII 765 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 152 CCACGG C^C[GA][CT]GG
EarI 544 GAAGAG CTCTTC(1/4)
EcoT14I 3177 CCTAGG C^C[AT][AT]GG
HaeII 2115 GGCGCC [GA]GCGC^[CT]
Hin1I 2115 GGCGCC G[GA]^CG[CT]C
HpaI 3204 GTTAAC GTT^AAC
KasI 2115 GGCGCC G^GCGCC
Ksp632I 544 GAAGAG CTCTTC(1/4)
MflI 2447 AGATCT [GA]^GATC[CT]
MslI 1627 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2115 GGCGCC GG^CGCC
NdeI 477 CATATG CA^TATG
PflMI 644 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1048 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2494 ATTAAT AT^TAAT
SalI 1315 GTCGAC G^TCGAC
ScaI 2803 AGTACT AGT^ACT
SfoI 2115 GGCGCC GGC^GCC
SmaI 2063 CCCGGG CCC^GGG
SmlI 2416 CTTAAG C^T[CT][GA]AG
SnaBI 2553 TACGTA TAC^GTA
SphI 1511 GCATGC GCATG^C
StuI 755 AGGCCT AGG^CCT
StyI 3177 CCTAGG C^C[AT][AT]GG
Van91I 644 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2447 AGATCT [GA]^GATC[CT]
XmaCI 2063 CCCGGG C^CCGGG
XmaI 2063 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 321 GGTACC G^GTACC
1888 GGTACC
AccIII 276 TCCGGA T^CCGGA
676 TCCGGA
AlwI 1263 GATCC GGATC(4/5)
2208 GATCC
Asp718I 321 GGTACC G^GTACC
1888 GGTACC
BclI 2562 TGATCA T^GATCA
2572 TGATCA
BsaI 2186 GGTCTC GGTCTC(1/5)
3563 GGTCTC
BsaWI 276 TCCGGA [AT]^CCGG[AT]
676 TCCGGA
BseAI 276 TCCGGA T^CCGGA
676 TCCGGA
BsgI 705 CTGCAC GTGCAG(16/14)
771 GTGCAG
BspEI 276 TCCGGA T^CCGGA
676 TCCGGA
BsrBI 69 GAGCGG CCGCTC(-3/-3)
79 GAGCGG
BssHII 24 GCGCGC G^CGCGC
26 GCGCGC
Bst1107I 1090 GTATAC GTA^TAC
3540 GTATAC
BstZ17I 1090 GTATAC GTA^TAC
3540 GTATAC
DraII 146 GGGCCCC [GA]G^G[ACGT]CC[CT]
3173 AGGCCCT
EcoO109I 146 GGGCCCC [GA]G^G[ACGT]CC[CT]
3173 AGGCCCT
EcoT22I 1459 ATGCAT ATGCA^T
2867 ATGCAT
FbaI 2562 TGATCA T^GATCA
2572 TGATCA
HgaI 223 GACGC GACGC(5/10)
823 GACGC
KpnI 321 GGTACC GGTAC^C
1888 GGTACC
MroI 276 TCCGGA T^CCGGA
676 TCCGGA
NsiI 1459 ATGCAT ATGCA^T
2867 ATGCAT
PvuII 1710 CAGCTG CAG^CTG
2466 CAGCTG
SfcI 660 CTACAG C^T[GA][CT]AG
1420 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI AvaI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I