##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BbrPI BbsI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FauI FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 405 CGCG CG^CG
AhdI 2568 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1377 CCTTAGG CC^T[ACGT]AGG
AspEI 2568 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 2243 GGACC G^G[AT]CC
AviII 1953 TGCGCA TGC^GCA
BbvCI 2784 CCTCAGC CCTCAGC(-5/-2)
BcgI 2773 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2218 TGATCA T^GATCA
BsaBI 2892 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 3491 CCGCTC CCGCTC(-3/-3)
BssSI 2610 CACGAG CACGAG(-5/-1)
BstAPI 459 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 3216 CATCC GGATG(2/0)
BstUI 405 CGCG CG^CG
BstXI 3188 CCAGTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2798 GGATCT [GA]^GATC[CT]
Bsu36I 1377 CCTTAGG CC^T[ACGT]AGG
DraIII 88 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3291 GACCCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2948 CCGTGG C^C[GA][CT]GG
Eam1105I 2568 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1377 CCTTAGG CC^T[ACGT]AGG
FbaI 2218 TGATCA T^GATCA
FokI 3216 CATCC GGATG(9/13)
FspI 1953 TGCGCA TGC^GCA
HgaI 872 GCGTC GACGC(5/10)
MamI 2892 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2798 GGATCT [GA]^GATC[CT]
MspA1I 750 CAGCTG C[AC]G^C[GT]G
MvnI 405 CGCG CG^CG
NdeI 1583 CATATG CA^TATG
PaeR7I 1982 CTCGAG C^TCGAG
PmeI 124 GTTTAAAC GTTT^AAAC
PpuMI 2242 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 750 CAGCTG CAG^CTG
SmaI 2793 CCCGGG CCC^GGG
SpeI 1234 ACTAGT A^CTAGT
SphI 3449 GCATGC GCATG^C
SspI 2423 AATATT AAT^ATT
XbaI 817 TCTAGA T^CTAGA
XhoI 1982 CTCGAG C^TCGAG
XhoII 2798 GGATCT [GA]^GATC[CT]
XmaCI 2793 CCCGGG C^CCGGG
XmaI 2793 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 786 ACACGT A^C[GA][CT]GT
2411 ACACGT
AlwNI 460 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
2499 CAGCATCTG
AspHI 1788 GAGCTC G[AT]GC[AT]^C
2310 GAGCTC
AvaI 1982 CTCGAG C^[CT]CG[GA]G
2793 CCCGGG
BalI 2685 TGGCCA TGG^CCA
3348 TGGCCA
BanI 303 GGCACC G^G[CT][GA]CC
1288 GGCACC
BanII 1788 GAGCTC G[GA]GC[CT]^C
2310 GAGCTC
BsaAI 787 CACGTA [CT]AC^GT[GA]
2412 CACGTA
BsiHKAI 1788 GAGCTC G[AT]GC[AT]^C
2310 GAGCTC
BsmI 760 GAATGC GAATGC(1/-1)
1915 GAATGC
BsoBI 1982 CTCGAG C^[CT]CG[GA]G
2793 CCCGGG
Bsp1407I 2408 TGTACA T^GTACA
3163 TGTACA
BspMI 361 GCAGGT ACCTGC(4/8)
2616 GCAGGT
BsrGI 2408 TGTACA T^GTACA
3163 TGTACA
CfoI 404 GCGC GCG^C
1954 GCGC
DraI 125 TTTAAA TTT^AAA
1960 TTTAAA
EcoNI 204 CCTTAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
355 CCTGGGGCAGG
EcoT22I 762 ATGCAT ATGCA^T
2854 ATGCAT
HapII 2552 CCGG C^CGG
2794 CCGG
HhaI 404 GCGC GCG^C
1954 GCGC
HinP1I 404 GCGC G^CGC
1954 GCGC
HpaII 2552 CCGG C^CGG
2794 CCGG
MluNI 2685 TGGCCA TGG^CCA
3348 TGGCCA
MscI 2685 TGGCCA TGG^CCA
3348 TGGCCA
MspI 2552 CCGG C^CGG
2794 CCGG
NsiI 762 ATGCAT ATGCA^T
2854 ATGCAT
NspI 2903 GCATGT [GA]CATG^[CT]
3449 GCATGC
SacI 1788 GAGCTC GAGCT^C
2310 GAGCTC
SspBI 2408 TGTACA T^GTACA
3163 TGTACA
TfiI 525 GAATC G^A[AT]TC
2774 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI