##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AccIII AgeI AhdI AlwNI Aor51HI
AscI AseI AsnI AspEI AvaI
AviII BamHI BbeI BglII BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HincII HindII HpaI
KasI KspI MfeI MflI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1495 GACGTC GACGT^C
Acc65I 2309 GGTACC G^GTACC
AccI 1905 GTAGAC GT^[AC][GT]AC
AccII 194 CGCG CG^CG
AclI 2818 AACGTT AA^CGTT
AcyI 1495 GACGTC G[GA]^CG[CT]C
AflII 2062 CTTAAG C^TTAAG
Alw44I 341 GTGCAC G^TGCAC
AlwI 2327 GATCC GGATC(4/5)
AocI 1262 CCTCAGG CC^T[ACGT]AGG
ApaLI 341 GTGCAC G^TGCAC
Asp718I 2309 GGTACC G^GTACC
AspI 3350 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 590 TGGCCA TGG^CCA
BbrPI 131 CACGTG CAC^GTG
BcgI 308 CGACGTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 841 TGATCA T^GATCA
BfrI 2062 CTTAAG C^TTAAG
BlpI 2257 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2257 GCTGAGC GC^T[ACGT]AGC
BsaAI 131 CACGTG [CT]AC^GT[GA]
BsaBI 1310 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1495 GACGTC G[GA]^CG[CT]C
BsmBI 1497 CGTCTC CGTCTC(1/5)
BspLU11I 555 ACATGT A^CATGT
BstAPI 665 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 194 CGCG CG^CG
Bsu36I 1262 CCTCAGG CC^T[ACGT]AGG
CelII 2257 GCTGAGC GC^T[ACGT]AGC
DraIII 2891 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1855 GACAAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1262 CCTCAGG CC^T[ACGT]AGG
EcoRI 2279 GAATTC G^AATTC
EcoT22I 290 ATGCAT ATGCA^T
FbaI 841 TGATCA T^GATCA
HgaI 467 GACGC GACGC(5/10)
Hin1I 1495 GACGTC G[GA]^CG[CT]C
KpnI 2309 GGTACC GGTAC^C
MamI 1310 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 590 TGGCCA TGG^CCA
MscI 590 TGGCCA TGG^CCA
MvnI 194 CGCG CG^CG
NruI 193 TCGCGA TCG^CGA
NsiI 290 ATGCAT ATGCA^T
PflFI 3350 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 131 CACGTG CAC^GTG
PmlI 131 CACGTG CAC^GTG
Psp1406I 2818 AACGTT AA^CGTT
SapI 1828 GCTCTTC GCTCTTC(1/4)
ScaI 1671 AGTACT AGT^ACT
SfiI 1049 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 3350 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 1883 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2657 GAAGCAGTTC
AvrII 1548 CCTAGG C^CTAGG
3333 CCTAGG
BbsI 551 GAAGAC GAAGAC(2/6)
221 GTCTTC
BbvCI 3138 GCTGAGG CCTCAGC(-5/-2)
3271 GCTGAGG
BlnI 1548 CCTAGG C^CTAGG
3333 CCTAGG
BpuAI 551 GAAGAC GAAGAC(2/6)
221 GTCTTC
BsaWI 409 ACCGGA [AT]^CCGG[AT]
1227 ACCGGA
BsgI 3322 CTGCAC GTGCAG(16/14)
2274 GTGCAG
BsrDI 460 GCAATG GCAATG(2/0)
1188 GCAATG
DraI 1911 TTTAAA TTT^AAA
2192 TTTAAA
HaeII 9 GGCGCT [GA]GCGC^[CT]
1114 AGCGCC
HindIII 2466 AAGCTT A^AGCTT
3067 AAGCTT
MslI 1607 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3056 CACTACTGTG
NcoI 1011 CCATGG C^CATGG
1290 CCATGG
SmlI 1342 CTTGAG C^T[CT][GA]AG
2062 CTTAAG
SspI 1915 AATATT AAT^ATT
2187 AATATT
StuI 1551 AGGCCT AGG^CCT
2621 AGGCCT
Tsp45I 742 GTGAC ^GT[GC]AC
1387 GTCAC
XcmI 73 CCAGGGGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2873 CCACTTTCTCACTGG
XmnI 1883 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2657 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApaI ApoI AspHI AvaII BanI
BanII BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I