##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BciVI       BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspHI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO109I    EcoO65I     
FseI        FspI        HindIII     HpaI        KpnI        
MluI        MroI        NaeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SgrAI       SmaI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         3015  GTCTAC               GT^[AC][GT]AC
AflIII        712  ACATGT               A^C[GA][CT]GT
AlwNI        1754  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1553  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       175  AGCGCT               AGC^GCT
AvaI         1459  CTCGGG               C^[CT]CG[GA]G
BanI          393  GGCGCC               G^G[CT][GA]CC
BanII          71  GAGCTC               G[GA]GC[CT]^C
BbeI          393  GGCGCC               GGCGC^C
BbsI         2303  GTCTTC               GAAGAC(2/6)
BcgI          731  CGAGGATTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2785  TGATCA               T^GATCA
BglII        1781  AGATCT               A^GATCT
BpuAI        2303  GTCTTC               GAAGAC(2/6)
BsaAI         694  TACGTA               [CT]AC^GT[GA]
BsaBI         302  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI          47  GAGACG               CGTCTC(1/5)
BsmI         1214  GAATGC               GAATGC(1/-1)
BsoBI        1459  CTCGGG               C^[CT]CG[GA]G
BspLU11I      712  ACATGT               A^CATGT
BspMI         190  GCAGGT               ACCTGC(4/8)
BsrDI        1022  CATTGC               GCAATG(2/0)
BstBI         814  TTCGAA               TT^CGAA
Bsu36I       1553  CCTAAGG              CC^T[ACGT]AGG
Eco47III      175  AGCGCT               AGC^GCT
Eco81I       1553  CCTAAGG              CC^T[ACGT]AGG
EcoNI          53  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         414  GATATC               GAT^ATC
FbaI         2785  TGATCA               T^GATCA
KasI          393  GGCGCC               G^GCGCC
KspI          163  CCGCGG               CCGC^GG
MamI          302  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2201  CAATTG               C^AATTG
MunI         2201  CAATTG               C^AATTG
NarI          393  GGCGCC               GG^CGCC
NspV          814  TTCGAA               TT^CGAA
PmeI         3334  GTTTAAAC             GTTT^AAAC
SacI           71  GAGCTC               GAGCT^C
SacII         163  CCGCGG               CCGC^GG
SapI           32  GCTCTTC              GCTCTTC(1/4)
SfoI          393  GGCGCC               GGC^GCC
SfuI          814  TTCGAA               TT^CGAA
SnaBI         694  TACGTA               TAC^GTA
XcmI          373  CCATCGTTCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          134  GATCC                GGATC(4/5)
             3270  GGATC               
AspHI          71  GAGCTC               G[AT]GC[AT]^C
             3001  GAGCAC              
BalI          719  TGGCCA               TGG^CCA
             2323  TGGCCA              
BseRI        3543  CTCCTC               GAGGAG(10/8)
             1650  GAGGAG              
BsgI          285  CTGCAC               GTGCAG(16/14)
             2514  GTGCAG              
BsiHKAI        71  GAGCTC               G[AT]GC[AT]^C
             3001  GAGCAC              
EcoT22I      1593  ATGCAT               ATGCA^T
             2179  ATGCAT              
FauI          211  CCCGC                CCCGC(4/6)
               93  GCGGG               
HincII        609  GTTGAC               GT[CT]^[GA]AC
             1663  GTCAAC              
HindII        609  GTTGAC               GT[CT]^[GA]AC
             1663  GTCAAC              
MluNI         719  TGGCCA               TGG^CCA
             2323  TGGCCA              
MscI          719  TGGCCA               TGG^CCA
             2323  TGGCCA              
NsiI         1593  ATGCAT               ATGCA^T
             2179  ATGCAT              
NspI          602  ACATGC               [GA]CATG^[CT]
              712  ACATGT              
PleI         1144  GACTC                GAGTC(4/5)
             1938  GAGTC               
PvuII        1545  CAGCTG               CAG^CTG
             3329  CAGCTG              
SpeI         1352  ACTAGT               A^CTAGT
             3464  ACTAGT              
XbaI         1380  TCTAGA               T^CTAGA
             1787  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflII       AluI        ApoI        AseI        AsnI        
Asp700I     AvaII       BbvI        BcnI        BfaI        
BfrI        BmyI        BsaHI       BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DsaI        EaeI        
EarI        EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI