################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BglI BlnI BlpI BpmI Bpu1102I BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO109I EcoO65I FseI FspI HindIII HpaI KpnI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SgrAI SmaI SphI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 3015 GTCTAC GT^[AC][GT]AC AflIII 712 ACATGT A^C[GA][CT]GT AlwNI 1754 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1553 CCTAAGG CC^T[ACGT]AGG Aor51HI 175 AGCGCT AGC^GCT AvaI 1459 CTCGGG C^[CT]CG[GA]G BanI 393 GGCGCC G^G[CT][GA]CC BanII 71 GAGCTC G[GA]GC[CT]^C BbeI 393 GGCGCC GGCGC^C BbsI 2303 GTCTTC GAAGAC(2/6) BcgI 731 CGAGGATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2785 TGATCA T^GATCA BglII 1781 AGATCT A^GATCT BpuAI 2303 GTCTTC GAAGAC(2/6) BsaAI 694 TACGTA [CT]AC^GT[GA] BsaBI 302 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 47 GAGACG CGTCTC(1/5) BsmI 1214 GAATGC GAATGC(1/-1) BsoBI 1459 CTCGGG C^[CT]CG[GA]G BspLU11I 712 ACATGT A^CATGT BspMI 190 GCAGGT ACCTGC(4/8) BsrDI 1022 CATTGC GCAATG(2/0) BstBI 814 TTCGAA TT^CGAA Bsu36I 1553 CCTAAGG CC^T[ACGT]AGG Eco47III 175 AGCGCT AGC^GCT Eco81I 1553 CCTAAGG CC^T[ACGT]AGG EcoNI 53 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 414 GATATC GAT^ATC FbaI 2785 TGATCA T^GATCA KasI 393 GGCGCC G^GCGCC KspI 163 CCGCGG CCGC^GG MamI 302 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2201 CAATTG C^AATTG MunI 2201 CAATTG C^AATTG NarI 393 GGCGCC GG^CGCC NspV 814 TTCGAA TT^CGAA PmeI 3334 GTTTAAAC GTTT^AAAC SacI 71 GAGCTC GAGCT^C SacII 163 CCGCGG CCGC^GG SapI 32 GCTCTTC GCTCTTC(1/4) SfoI 393 GGCGCC GGC^GCC SfuI 814 TTCGAA TT^CGAA SnaBI 694 TACGTA TAC^GTA XcmI 373 CCATCGTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 134 GATCC GGATC(4/5) 3270 GGATC AspHI 71 GAGCTC G[AT]GC[AT]^C 3001 GAGCAC BalI 719 TGGCCA TGG^CCA 2323 TGGCCA BseRI 3543 CTCCTC GAGGAG(10/8) 1650 GAGGAG BsgI 285 CTGCAC GTGCAG(16/14) 2514 GTGCAG BsiHKAI 71 GAGCTC G[AT]GC[AT]^C 3001 GAGCAC EcoT22I 1593 ATGCAT ATGCA^T 2179 ATGCAT FauI 211 CCCGC CCCGC(4/6) 93 GCGGG HincII 609 GTTGAC GT[CT]^[GA]AC 1663 GTCAAC HindII 609 GTTGAC GT[CT]^[GA]AC 1663 GTCAAC MluNI 719 TGGCCA TGG^CCA 2323 TGGCCA MscI 719 TGGCCA TGG^CCA 2323 TGGCCA NsiI 1593 ATGCAT ATGCA^T 2179 ATGCAT NspI 602 ACATGC [GA]CATG^[CT] 712 ACATGT PleI 1144 GACTC GAGTC(4/5) 1938 GAGTC PvuII 1545 CAGCTG CAG^CTG 3329 CAGCTG SpeI 1352 ACTAGT A^CTAGT 3464 ACTAGT XbaI 1380 TCTAGA T^CTAGA 1787 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AluI ApoI AseI AsnI Asp700I AvaII BbvI BcnI BfaI BfrI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI