##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BciVI BglI
BlnI BlpI BpmI Bpu1102I BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
FseI FspI HindIII HpaI KpnI
MluI MroI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfcI SfiI SgrAI SmaI SphI
Sse8387I StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 3015 GTCTAC GT^[AC][GT]AC
AflIII 712 ACATGT A^C[GA][CT]GT
AlwNI 1754 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1553 CCTAAGG CC^T[ACGT]AGG
Aor51HI 175 AGCGCT AGC^GCT
AvaI 1459 CTCGGG C^[CT]CG[GA]G
BanI 393 GGCGCC G^G[CT][GA]CC
BanII 71 GAGCTC G[GA]GC[CT]^C
BbeI 393 GGCGCC GGCGC^C
BbsI 2303 GTCTTC GAAGAC(2/6)
BcgI 731 CGAGGATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2785 TGATCA T^GATCA
BglII 1781 AGATCT A^GATCT
BpuAI 2303 GTCTTC GAAGAC(2/6)
BsaAI 694 TACGTA [CT]AC^GT[GA]
BsaBI 302 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 47 GAGACG CGTCTC(1/5)
BsmI 1214 GAATGC GAATGC(1/-1)
BsoBI 1459 CTCGGG C^[CT]CG[GA]G
BspLU11I 712 ACATGT A^CATGT
BspMI 190 GCAGGT ACCTGC(4/8)
BsrDI 1022 CATTGC GCAATG(2/0)
BstBI 814 TTCGAA TT^CGAA
Bsu36I 1553 CCTAAGG CC^T[ACGT]AGG
Eco47III 175 AGCGCT AGC^GCT
Eco81I 1553 CCTAAGG CC^T[ACGT]AGG
EcoNI 53 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 414 GATATC GAT^ATC
FbaI 2785 TGATCA T^GATCA
KasI 393 GGCGCC G^GCGCC
KspI 163 CCGCGG CCGC^GG
MamI 302 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2201 CAATTG C^AATTG
MunI 2201 CAATTG C^AATTG
NarI 393 GGCGCC GG^CGCC
NspV 814 TTCGAA TT^CGAA
PmeI 3334 GTTTAAAC GTTT^AAAC
SacI 71 GAGCTC GAGCT^C
SacII 163 CCGCGG CCGC^GG
SapI 32 GCTCTTC GCTCTTC(1/4)
SfoI 393 GGCGCC GGC^GCC
SfuI 814 TTCGAA TT^CGAA
SnaBI 694 TACGTA TAC^GTA
XcmI 373 CCATCGTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 134 GATCC GGATC(4/5)
3270 GGATC
AspHI 71 GAGCTC G[AT]GC[AT]^C
3001 GAGCAC
BalI 719 TGGCCA TGG^CCA
2323 TGGCCA
BseRI 3543 CTCCTC GAGGAG(10/8)
1650 GAGGAG
BsgI 285 CTGCAC GTGCAG(16/14)
2514 GTGCAG
BsiHKAI 71 GAGCTC G[AT]GC[AT]^C
3001 GAGCAC
EcoT22I 1593 ATGCAT ATGCA^T
2179 ATGCAT
FauI 211 CCCGC CCCGC(4/6)
93 GCGGG
HincII 609 GTTGAC GT[CT]^[GA]AC
1663 GTCAAC
HindII 609 GTTGAC GT[CT]^[GA]AC
1663 GTCAAC
MluNI 719 TGGCCA TGG^CCA
2323 TGGCCA
MscI 719 TGGCCA TGG^CCA
2323 TGGCCA
NsiI 1593 ATGCAT ATGCA^T
2179 ATGCAT
NspI 602 ACATGC [GA]CATG^[CT]
712 ACATGT
PleI 1144 GACTC GAGTC(4/5)
1938 GAGTC
PvuII 1545 CAGCTG CAG^CTG
3329 CAGCTG
SpeI 1352 ACTAGT A^CTAGT
3464 ACTAGT
XbaI 1380 TCTAGA T^CTAGA
1787 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI ApoI AseI AsnI
Asp700I AvaII BbvI BcnI BfaI
BfrI BmyI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI