##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        Asp700I     Asp718I     
AspI        AvrII       BbrPI       BfrI        BlnI        
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SapI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         783  GACGTC               GACGT^C
AcsI         1463  GAATTC               [GA]^AATT[CT]
AhdI         3411  GACCCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2035  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1483  AGCGCT               AGC^GCT
ApoI         1463  GAATTC               [GA]^AATT[CT]
AspEI        3411  GACCCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         319  TGCGCA               TGC^GCA
BamHI        1275  GGATCC               G^GATCC
BbeI         1248  GGCGCC               GGCGC^C
BcgI          994  GCAAGATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          106  TGATCA               T^GATCA
BglI         2072  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI          88  TCCGGT               [AT]^CCGG[AT]
BsrFI        1312  GCCGGC               [GA]^CCGG[CT]
Bsu36I       2035  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1312  GCCGGC               [GA]^CCGG[CT]
DraI         3499  TTTAAA               TTT^AAA
Eam1105I     3411  GACCCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1483  AGCGCT               AGC^GCT
Eco81I       2035  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1463  GAATTC               G^AATTC
FbaI          106  TGATCA               T^GATCA
FspI          319  TGCGCA               TGC^GCA
KasI         1248  GGCGCC               G^GCGCC
NaeI         1312  GCCGGC               GCC^GGC
NarI         1248  GGCGCC               GG^CGCC
NgoMIV       1312  GCCGGC               G^CCGGC
NruI         1043  TCGCGA               TCG^CGA
PflMI         863  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1591  GAGCTC               GAGCT^C
SalI         2248  GTCGAC               G^TCGAC
SbfI         2575  CCTGCAGG             CCTGCA^GG
SexAI         324  ACCTGGT              A^CC[AT]GGT
SfoI         1248  GGCGCC               GGC^GCC
SphI          775  GCATGC               GCATG^C
Sse8387I     2575  CCTGCAGG             CCTGCA^GG
Van91I        863  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          667  CCATCAACCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII        922  ACATGT               A^C[GA][CT]GT
             2131  ACATGT              
AseI         2882  ATTAAT               AT^TAAT
             3017  ATTAAT              
AsnI         2882  ATTAAT               AT^TAAT
             3017  ATTAAT              
BciVI        2687  GTATCC               GTATCC(6/5)
             3283  GTATCC              
BglII         520  AGATCT               A^GATCT
             1291  AGATCT              
BspLU11I      922  ACATGT               A^CATGT
             2131  ACATGT              
DrdI          402  GACGCTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              654  GACTGCTGGGTC        
HgaI          402  GACGC                GACGC(5/10)
              884  GCGTC               
HincII        336  GTTGAC               GT[CT]^[GA]AC
             2248  GTCGAC              
HindII        336  GTTGAC               GT[CT]^[GA]AC
             2248  GTCGAC              
KspI          294  CCGCGG               CCGC^GG
             1244  CCGCGG              
MaeII         202  ACGT                 A^CGT
              784  ACGT                
PshBI        2882  ATTAAT               AT^TAAT
             3017  ATTAAT              
PvuII        1651  CAGCTG               CAG^CTG
             3491  CAGCTG              
SacII         294  CCGCGG               CCGC^GG
             1244  CCGCGG              
SmlI         1389  CTTGAG               C^T[CT][GA]AG
             2305  CTCAAG              
TfiI         2310  GAATC                G^A[AT]TC
             2928  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmaI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmaCI       
XmaI