################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I ApaLI AscI Asp700I Asp718I AspI AvrII BbrPI BfrI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SapI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 783 GACGTC GACGT^C AcsI 1463 GAATTC [GA]^AATT[CT] AhdI 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2035 CCTCAGG CC^T[ACGT]AGG Aor51HI 1483 AGCGCT AGC^GCT ApoI 1463 GAATTC [GA]^AATT[CT] AspEI 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 319 TGCGCA TGC^GCA BamHI 1275 GGATCC G^GATCC BbeI 1248 GGCGCC GGCGC^C BcgI 994 GCAAGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 106 TGATCA T^GATCA BglI 2072 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 88 TCCGGT [AT]^CCGG[AT] BsrFI 1312 GCCGGC [GA]^CCGG[CT] Bsu36I 2035 CCTCAGG CC^T[ACGT]AGG Cfr10I 1312 GCCGGC [GA]^CCGG[CT] DraI 3499 TTTAAA TTT^AAA Eam1105I 3411 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1483 AGCGCT AGC^GCT Eco81I 2035 CCTCAGG CC^T[ACGT]AGG EcoRI 1463 GAATTC G^AATTC FbaI 106 TGATCA T^GATCA FspI 319 TGCGCA TGC^GCA KasI 1248 GGCGCC G^GCGCC NaeI 1312 GCCGGC GCC^GGC NarI 1248 GGCGCC GG^CGCC NgoMIV 1312 GCCGGC G^CCGGC NruI 1043 TCGCGA TCG^CGA PflMI 863 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1591 GAGCTC GAGCT^C SalI 2248 GTCGAC G^TCGAC SbfI 2575 CCTGCAGG CCTGCA^GG SexAI 324 ACCTGGT A^CC[AT]GGT SfoI 1248 GGCGCC GGC^GCC SphI 775 GCATGC GCATG^C Sse8387I 2575 CCTGCAGG CCTGCA^GG Van91I 863 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 667 CCATCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 922 ACATGT A^C[GA][CT]GT 2131 ACATGT AseI 2882 ATTAAT AT^TAAT 3017 ATTAAT AsnI 2882 ATTAAT AT^TAAT 3017 ATTAAT BciVI 2687 GTATCC GTATCC(6/5) 3283 GTATCC BglII 520 AGATCT A^GATCT 1291 AGATCT BspLU11I 922 ACATGT A^CATGT 2131 ACATGT DrdI 402 GACGCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 654 GACTGCTGGGTC HgaI 402 GACGC GACGC(5/10) 884 GCGTC HincII 336 GTTGAC GT[CT]^[GA]AC 2248 GTCGAC HindII 336 GTTGAC GT[CT]^[GA]AC 2248 GTCGAC KspI 294 CCGCGG CCGC^GG 1244 CCGCGG MaeII 202 ACGT A^CGT 784 ACGT PshBI 2882 ATTAAT AT^TAAT 3017 ATTAAT PvuII 1651 CAGCTG CAG^CTG 3491 CAGCTG SacII 294 CCGCGG CCGC^GG 1244 CCGCGG SmlI 1389 CTTGAG C^T[CT][GA]AG 2305 CTCAAG TfiI 2310 GAATC G^A[AT]TC 2928 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI