##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1829 GTATAC GT^[AC][GT]AC
AspHI 511 GTGCTC G[AT]GC[AT]^C
BalI 791 TGGCCA TGG^CCA
BanII 553 GGGCTC G[GA]GC[CT]^C
BbrPI 1260 CACGTG CAC^GTG
BseRI 664 CTCCTC GAGGAG(10/8)
BsgI 495 GTGCAG GTGCAG(16/14)
BsiHKAI 511 GTGCTC G[AT]GC[AT]^C
BsiYI 794 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 794 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 527 ACATGT A^CATGT
BsrDI 315 CATTGC GCAATG(2/0)
Bst1107I 1829 GTATAC GTA^TAC
BstXI 1520 CCACTAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1829 GTATAC GTA^TAC
EaeI 791 TGGCCA [CT]^GGCC[GA]
FauI 319 GCGGG CCCGC(4/6)
HgaI 78 GACGC GACGC(5/10)
HincII 523 GTCAAC GT[CT]^[GA]AC
HindII 523 GTCAAC GT[CT]^[GA]AC
MluNI 791 TGGCCA TGG^CCA
MscI 791 TGGCCA TGG^CCA
PflMI 794 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1260 CACGTG CAC^GTG
PmlI 1260 CACGTG CAC^GTG
PshAI 1908 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 513 GCTCTTC GCTCTTC(1/4)
SpeI 1522 ACTAGT A^CTAGT
SwaI 1547 ATTTAAAT ATTT^AAAT
Tsp45I 455 GTGAC ^GT[GC]AC
Van91I 794 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 447 GATCC GGATC(4/5)
84 GGATC
Asp700I 1044 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2305 GAAGACATTC
AvaII 469 GGTCC G^G[AT]CC
1086 GGACC
BbsI 2305 GAAGAC GAAGAC(2/6)
310 GTCTTC
BclI 218 TGATCA T^GATCA
272 TGATCA
BpmI 242 CTCCAG CTGGAG(16/14)
2232 CTGGAG
BpuAI 2305 GAAGAC GAAGAC(2/6)
310 GTCTTC
BsaAI 361 TACGTG [CT]AC^GT[GA]
1260 CACGTG
BssKI 473 CCTGG ^CC[ACGT]GG
1928 CCTGG
BstNI 473 CCTGG CC^[AT]GG
1928 CCTGG
BstYI 84 GGATCT [GA]^GATC[CT]
446 AGATCC
Cac8I 1112 GCCAGC GC[ACGT]^[ACGT]GC
1669 GCAGGC
DraI 1548 TTTAAA TTT^AAA
2138 TTTAAA
EcoRI 882 GAATTC G^AATTC
1048 GAATTC
EcoRII 473 CCTGG ^CC[AT]GG
1928 CCTGG
FbaI 218 TGATCA T^GATCA
272 TGATCA
HphI 454 GGTGA GGTGA(8/7)
692 TCACC
MflI 84 GGATCT [GA]^GATC[CT]
446 AGATCC
MslI 288 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CATCTTCGTG
MspA1I 478 CAGCTG C[AC]G^C[GT]G
1011 CAGCTG
MvaI 473 CCTGG CC^[AT]GG
1928 CCTGG
MwoI 129 GCTCACTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1742 GCAGGAAAAGC
PvuII 478 CAGCTG CAG^CTG
1011 CAGCTG
ScrFI 473 CCTGG CC^[ACGT]GG
1928 CCTGG
XhoII 84 GGATCT [GA]^GATC[CT]
446 AGATCC
XmnI 1044 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2305 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI ApoI AseI AsnI BbvI
BfaI BfrI BmyI BsaI BsaJI
BsmAI BsmFI Bsp1286I BsrI BstF5I
Cfr13I DdeI DpnII EarI EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I SfaNI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp509I TspRI