##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AvaII
AviII AvrII BanII BbeI BbrPI
BbvCI BciVI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HhaI
HinP1I KasI KspI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NdeI NgoMIV NheI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 638 GGTACC G^GTACC
AccII 19 CGCG CG^CG
AcyI 4427 GGCGTC G[GA]^CG[CT]C
Asp718I 638 GGTACC G^GTACC
AspI 3878 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 325 CTCGAG C^[CT]CG[GA]G
BalI 1651 TGGCCA TGG^CCA
BamHI 868 GGATCC G^GATCC
BanI 638 GGTACC G^G[CT][GA]CC
BcgI 555 GCAGCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 4427 GGCGTC G[GA]^CG[CT]C
BsoBI 325 CTCGAG C^[CT]CG[GA]G
Bst1107I 1363 GTATAC GTA^TAC
BstUI 19 CGCG CG^CG
BstXI 872 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1363 GTATAC GTA^TAC
Cfr13I 2048 GGCCC G^G[ACGT]CC
DraII 2047 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 82 CCATGG C^C[GA][CT]GG
EcoO109I 2047 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2288 ATGCAT ATGCA^T
Hin1I 4427 GGCGTC G[GA]^CG[CT]C
HincII 1154 GTTAAC GT[CT]^[GA]AC
HindII 1154 GTTAAC GT[CT]^[GA]AC
HpaI 1154 GTTAAC GTT^AAC
KpnI 638 GGTACC GGTAC^C
MaeII 1961 ACGT A^CGT
MluNI 1651 TGGCCA TGG^CCA
MscI 1651 TGGCCA TGG^CCA
MvnI 19 CGCG CG^CG
NcoI 82 CCATGG C^CATGG
NotI 14 GCGGCCGC GC^GGCCGC
NsiI 2288 ATGCAT ATGCA^T
PaeR7I 325 CTCGAG C^TCGAG
PflFI 3878 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 804 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1417 CTGCAG CTGCA^G
Sau96I 2048 GGCCC G^G[ACGT]CC
SexAI 3970 ACCAGGT A^CC[AT]GGT
StuI 2845 AGGCCT AGG^CCT
Tth111I 3878 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3347 TCTAGA T^CTAGA
XcmI 2492 CCATGAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 325 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflII 1992 CTTAAG C^TTAAG
2478 CTTAAG
AspHI 3351 GAGCAC G[AT]GC[AT]^C
3963 GAGCAC
BclI 365 TGATCA T^GATCA
2444 TGATCA
BcnI 27 CCCGG CC^[GC]GG
40 CCCGG
BfrI 1992 CTTAAG C^TTAAG
2478 CTTAAG
BlpI 1518 GCTGAGC GC^T[ACGT]AGC
2433 GCTAAGC
BmyI 3351 GAGCAC G[AGT]GC[ACT]^C
3963 GAGCAC
Bpu1102I 1518 GCTGAGC GC^T[ACGT]AGC
2433 GCTAAGC
BsaI 30 GGTCTC GGTCTC(1/5)
3111 GGTCTC
BseRI 2370 GAGGAG GAGGAG(10/8)
3333 GAGGAG
BsgI 2074 CTGCAC GTGCAG(16/14)
2034 GTGCAG
BsiEI 15 CGGCCG CG[GA][CT]^CG
42 CGGCCG
BsiHKAI 3351 GAGCAC G[AT]GC[AT]^C
3963 GAGCAC
BsiYI 2755 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3851 CCCAGAAACGG
BslI 2755 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3851 CCCAGAAACGG
Bsp1286I 3351 GAGCAC G[AGT]GC[ACT]^C
3963 GAGCAC
Bsp1407I 2978 TGTACA T^GTACA
4331 TGTACA
BspHI 166 TCATGA T^CATGA
3062 TCATGA
BspMI 464 GCAGGT ACCTGC(4/8)
1790 GCAGGT
BsrDI 661 CATTGC GCAATG(2/0)
1189 GCAATG
BsrGI 2978 TGTACA T^GTACA
4331 TGTACA
BstYI 868 GGATCC [GA]^GATC[CT]
2777 GGATCT
CelII 1518 GCTGAGC GC^T[ACGT]AGC
2433 GCTAAGC
EagI 15 CGGCCG C^GGCCG
42 CGGCCG
EclXI 15 CGGCCG C^GGCCG
42 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
42 CGGCCG
EcoT14I 82 CCATGG C^C[AT][AT]GG
1699 CCTTGG
FbaI 365 TGATCA T^GATCA
2444 TGATCA
HapII 28 CCGG C^CGG
41 CCGG
HpaII 28 CCGG C^CGG
41 CCGG
MflI 868 GGATCC [GA]^GATC[CT]
2777 GGATCT
MspI 28 CCGG C^CGG
41 CCGG
NciI 27 CCCGG CC^[GC]GG
40 CCCGG
NlaIV 638 GGTACC GG[ACGT]^[ACGT]CC
868 GGATCC
PvuII 556 CAGCTG CAG^CTG
2964 CAGCTG
RcaI 166 TCATGA T^CATGA
3062 TCATGA
SspBI 2978 TGTACA T^GTACA
4331 TGTACA
StyI 82 CCATGG C^C[AT][AT]GG
1699 CCTTGG
XhoII 868 GGATCC [GA]^GATC[CT]
2777 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsmAI BsmFI BsmI BspLU11I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII DraI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HgaI
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NspI PleI RsaI SapI
Sau3AI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI