##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI Asp718I AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 1875 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 552 GTGCAC G^TGCAC
ApaLI 552 GTGCAC G^TGCAC
Asp700I 518 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1875 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1571 GGCACC G^G[CT][GA]CC
BanII 1785 GGGCTC G[GA]GC[CT]^C
BbvCI 2129 CCTCAGC CCTCAGC(-5/-2)
BcgI 2971 GCATGACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1811 GGATAC GTATCC(6/5)
BclI 462 TGATCA T^GATCA
BglI 627 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmFI 1869 GGGAC GGGAC(10/14)
Bsp1407I 1513 TGTACA T^GTACA
BsrGI 1513 TGTACA T^GTACA
Eam1105I 1875 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1562 CCTAGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1158 GATATC GAT^ATC
EcoT14I 1248 CCAAGG C^C[AT][AT]GG
FbaI 462 TGATCA T^GATCA
HaeIII 2148 GGCC GG^CC
MaeII 535 ACGT A^CGT
MspA1I 2683 CAGCTG C[AC]G^C[GT]G
PvuII 2683 CAGCTG CAG^CTG
ScaI 1104 AGTACT AGT^ACT
SexAI 1470 ACCTGGT A^CC[AT]GGT
SmlI 3125 CTTGAG C^T[CT][GA]AG
SspBI 1513 TGTACA T^GTACA
StyI 1248 CCAAGG C^C[AT][AT]GG
XmnI 518 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 155 GTCTAC GT^[AC][GT]AC
311 GTAGAC
AciI 514 GCGG CCGC(-3/-1)
636 GCGG
AlwNI 1617 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2388 CAGAAACTG
BcnI 285 CCCGG CC^[GC]GG
2030 CCGGG
BglII 1662 AGATCT A^GATCT
3039 AGATCT
BstBI 255 TTCGAA TT^CGAA
417 TTCGAA
EcoRI 769 GAATTC G^AATTC
1282 GAATTC
FauI 514 GCGGG CCCGC(4/6)
636 GCGGG
HapII 286 CCGG C^CGG
2030 CCGG
HgaI 920 GACGC GACGC(5/10)
1433 GCGTC
HincII 829 GTTAAC GT[CT]^[GA]AC
1631 GTTAAC
HindII 829 GTTAAC GT[CT]^[GA]AC
1631 GTTAAC
HpaI 829 GTTAAC GTT^AAC
1631 GTTAAC
HpaII 286 CCGG C^CGG
2030 CCGG
MfeI 1306 CAATTG C^AATTG
3103 CAATTG
MspI 286 CCGG C^CGG
2030 CCGG
MunI 1306 CAATTG C^AATTG
3103 CAATTG
NciI 285 CCCGG CC^[GC]GG
2030 CCGGG
NdeI 1020 CATATG CA^TATG
1986 CATATG
NspV 255 TTCGAA TT^CGAA
417 TTCGAA
PflMI 1575 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1616 CCAGATCCTGG
PstI 107 CTGCAG CTGCA^G
588 CTGCAG
SapI 227 GAAGAGC GCTCTTC(1/4)
264 GAAGAGC
SfuI 255 TTCGAA TT^CGAA
417 TTCGAA
Van91I 1575 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1616 CCAGATCCTGG
XbaI 2021 TCTAGA T^CTAGA
2739 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AseI AsnI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII