################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AccIII AflIII AgeI AhdI ApaI AscI AspEI AspI AviII AvrII BbrPI BglII BlnI BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AccI 1154 GTAGAC GT^[AC][GT]AC AclI 3364 AACGTT AA^CGTT AflII 3506 CTTAAG C^TTAAG Alw44I 3255 GTGCAC G^TGCAC Aor51HI 115 AGCGCT AGC^GCT ApaLI 3255 GTGCAC G^TGCAC AspHI 3255 GTGCAC G[AT]GC[AT]^C BbeI 2466 GGCGCC GGCGC^C BciVI 1035 GTATCC GTATCC(6/5) BclI 1054 TGATCA T^GATCA BfrI 3506 CTTAAG C^TTAAG BsaWI 3533 TCCGGT [AT]^CCGG[AT] BsiHKAI 3255 GTGCAC G[AT]GC[AT]^C Eco47III 115 AGCGCT AGC^GCT EcoNI 3809 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1054 TGATCA T^GATCA KasI 2466 GGCGCC G^GCGCC NarI 2466 GGCGCC GG^CGCC NheI 3191 GCTAGC G^CTAGC NruI 6268 TCGCGA TCG^CGA PaeR7I 169 CTCGAG C^TCGAG PshAI 4771 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3364 AACGTT AA^CGTT SfoI 2466 GGCGCC GGC^GCC XhoI 169 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 3022 GACGTC GACGT^C 4084 GACGTC AccII 52 CGCG CG^CG 6269 CGCG AseI 1061 ATTAAT AT^TAAT 1265 ATTAAT AsnI 1061 ATTAAT AT^TAAT 1265 ATTAAT Asp700I 3987 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4610 GAACCTGTTC BsaBI 2114 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2531 GATCAGCATC BsrBI 6150 CCGCTC CCGCTC(-3/-3) 86 GAGCGG BstUI 52 CGCG CG^CG 6269 CGCG BstXI 5860 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6401 CCAGGGTTTTGG DrdI 2290 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4511 GACAGTGAGGTC HaeII 115 AGCGCT [GA]GCGC^[CT] 2466 GGCGCC HgaI 3749 GACGC GACGC(5/10) 1661 GCGTC HincII 1808 GTCAAC GT[CT]^[GA]AC 3454 GTTGAC HindII 1808 GTCAAC GT[CT]^[GA]AC 3454 GTTGAC MamI 2114 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2531 GATCAGCATC MvnI 52 CGCG CG^CG 6269 CGCG NcoI 543 CCATGG C^CATGG 1140 CCATGG PshBI 1061 ATTAAT AT^TAAT 1265 ATTAAT StuI 5175 AGGCCT AGG^CCT 6441 AGGCCT XbaI 2515 TCTAGA T^CTAGA 4543 TCTAGA XmnI 3987 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4610 GAACCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI Asp718I AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SpeI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII