##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AccIII      AflIII      AgeI        AhdI        ApaI        
AscI        AspEI       AspI        AviII       AvrII       
BbrPI       BglII       BlnI        BsaAI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HpaI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NotI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AccI         1154  GTAGAC               GT^[AC][GT]AC
AclI         3364  AACGTT               AA^CGTT
AflII        3506  CTTAAG               C^TTAAG
Alw44I       3255  GTGCAC               G^TGCAC
Aor51HI       115  AGCGCT               AGC^GCT
ApaLI        3255  GTGCAC               G^TGCAC
AspHI        3255  GTGCAC               G[AT]GC[AT]^C
BbeI         2466  GGCGCC               GGCGC^C
BciVI        1035  GTATCC               GTATCC(6/5)
BclI         1054  TGATCA               T^GATCA
BfrI         3506  CTTAAG               C^TTAAG
BsaWI        3533  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      3255  GTGCAC               G[AT]GC[AT]^C
Eco47III      115  AGCGCT               AGC^GCT
EcoNI        3809  CCTCCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1054  TGATCA               T^GATCA
KasI         2466  GGCGCC               G^GCGCC
NarI         2466  GGCGCC               GG^CGCC
NheI         3191  GCTAGC               G^CTAGC
NruI         6268  TCGCGA               TCG^CGA
PaeR7I        169  CTCGAG               C^TCGAG
PshAI        4771  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3364  AACGTT               AA^CGTT
SfoI         2466  GGCGCC               GGC^GCC
XhoI          169  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AatII        3022  GACGTC               GACGT^C
             4084  GACGTC              
AccII          52  CGCG                 CG^CG
             6269  CGCG                
AseI         1061  ATTAAT               AT^TAAT
             1265  ATTAAT              
AsnI         1061  ATTAAT               AT^TAAT
             1265  ATTAAT              
Asp700I      3987  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4610  GAACCTGTTC          
BsaBI        2114  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2531  GATCAGCATC          
BsrBI        6150  CCGCTC               CCGCTC(-3/-3)
               86  GAGCGG              
BstUI          52  CGCG                 CG^CG
             6269  CGCG                
BstXI        5860  CCAAGGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6401  CCAGGGTTTTGG        
DrdI         2290  GACCACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4511  GACAGTGAGGTC        
HaeII         115  AGCGCT               [GA]GCGC^[CT]
             2466  GGCGCC              
HgaI         3749  GACGC                GACGC(5/10)
             1661  GCGTC               
HincII       1808  GTCAAC               GT[CT]^[GA]AC
             3454  GTTGAC              
HindII       1808  GTCAAC               GT[CT]^[GA]AC
             3454  GTTGAC              
MamI         2114  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2531  GATCAGCATC          
MvnI           52  CGCG                 CG^CG
             6269  CGCG                
NcoI          543  CCATGG               C^CATGG
             1140  CCATGG              
PshBI        1061  ATTAAT               AT^TAAT
             1265  ATTAAT              
StuI         5175  AGGCCT               AGG^CCT
             6441  AGGCCT              
XbaI         2515  TCTAGA               T^CTAGA
             4543  TCTAGA              
XmnI         3987  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4610  GAACCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

Acc65I      AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp718I     AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SexAI       SfaNI       SfcI        SmlI        SpeI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII