################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsgI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI Bsu36I ClaI CpoI DrdI Eco47III Eco81I EcoRV FseI FspI HaeII HincII HindII HpaI KasI KpnI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfoI SfuI SmlI SnaBI SpeI Sse8387I SspBI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 2173 GTATAC GT^[AC][GT]AC AccIII 164 TCCGGA T^CCGGA AcyI 105 GGCGTC G[GA]^CG[CT]C AgeI 1772 ACCGGT A^CCGGT AhdI 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2265 GTGCAC G^TGCAC ApaLI 2265 GTGCAC G^TGCAC Asp700I 1008 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2465 GGATCC G^GATCC BanII 700 GAGCTC G[GA]GC[CT]^C BlpI 237 GCTCAGC GC^T[ACGT]AGC Bpu1102I 237 GCTCAGC GC^T[ACGT]AGC BsaBI 502 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 105 GGCGTC G[GA]^CG[CT]C BseAI 164 TCCGGA T^CCGGA BsiEI 217 CGGCCG CG[GA][CT]^CG BsmI 1482 GCATTC GAATGC(1/-1) BspEI 164 TCCGGA T^CCGGA BspHI 980 TCATGA T^CATGA BsrDI 776 GCAATG GCAATG(2/0) Bst1107I 2173 GTATAC GTA^TAC BstZ17I 2173 GTATAC GTA^TAC CelII 237 GCTCAGC GC^T[ACGT]AGC DraIII 1134 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 217 CGGCCG C^GGCCG Eam1105I 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 217 CGGCCG C^GGCCG Eco52I 217 CGGCCG C^GGCCG EcoRI 1070 GAATTC G^AATTC FauI 96 GCGGG CCCGC(4/6) Hin1I 105 GGCGTC G[GA]^CG[CT]C HindIII 846 AAGCTT A^AGCTT KspI 78 CCGCGG CCGC^GG MamI 502 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 164 TCCGGA T^CCGGA MslI 348 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 910 GCTAGC G^CTAGC PflFI 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1772 ACCGGT A^CCGGT PpuMI 44 GGGTCCC [GA]G^G[AT]CC[CT] PstI 895 CTGCAG CTGCA^G PvuII 994 CAGCTG CAG^CTG RcaI 980 TCATGA T^CATGA SacI 700 GAGCTC GAGCT^C SacII 78 CCGCGG CCGC^GG SapI 702 GCTCTTC GCTCTTC(1/4) ScaI 1112 AGTACT AGT^ACT SfiI 630 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1771 CACCGGTG C[GA]^CCGG[CT]G SmaI 40 CCCGGG CCC^GGG SphI 1437 GCATGC GCATG^C SspI 2238 AATATT AAT^ATT StuI 638 AGGCCT AGG^CCT Tth111I 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1378 CCAGAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 40 CCCGGG C^CCGGG XmaI 40 CCCGGG C^CCGGG XmnI 1008 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 40 CCCGGG C^[CT]CG[GA]G 57 CTCGGG BanI 1366 GGCACC G^G[CT][GA]CC 2322 GGTGCC BbsI 298 GAAGAC GAAGAC(2/6) 1420 GAAGAC BglII 1221 AGATCT A^GATCT 1382 AGATCT BpuAI 298 GAAGAC GAAGAC(2/6) 1420 GAAGAC BsaI 661 GGTCTC GGTCTC(1/5) 1738 GGTCTC BsaWI 164 TCCGGA [AT]^CCGG[AT] 1772 ACCGGT BseRI 718 CTCCTC GAGGAG(10/8) 1601 GAGGAG BsoBI 40 CCCGGG C^[CT]CG[GA]G 57 CTCGGG BspMI 2279 ACCTGC ACCTGC(4/8) 474 GCAGGT DraI 2475 TTTAAA TTT^AAA 2495 TTTAAA DraII 44 GGGTCCC [GA]G^G[ACGT]CC[CT] 1719 GGGGCCT EaeI 217 CGGCCG [CT]^GGCC[GA] 1691 TGGCCG EcoO109I 44 GGGTCCC [GA]G^G[ACGT]CC[CT] 1719 GGGGCCT EcoT22I 157 ATGCAT ATGCA^T 712 ATGCAT HgaI 106 GCGTC GACGC(5/10) 1696 GCGTC MaeII 1732 ACGT A^CGT 2456 ACGT NsiI 157 ATGCAT ATGCA^T 712 ATGCAT NspI 1437 GCATGC [GA]CATG^[CT] 1762 ACATGC SfcI 895 CTGCAG C^T[GA][CT]AG 1371 CTACAG XbaI 2092 TCTAGA T^CTAGA 2428 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BclI BcnI BfaI BglI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EarI EcoNI EcoO65I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII