##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BstAPI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1052 CGCG CG^CG
AcyI 1088 GGCGCC G[GA]^CG[CT]C
AhdI 132 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 235 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 132 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1088 GGCGCC GGCGC^C
BbrPI 719 CACGTG CAC^GTG
BbsI 1159 GAAGAC GAAGAC(2/6)
BpuAI 1159 GAAGAC GAAGAC(2/6)
BsaAI 719 CACGTG [CT]AC^GT[GA]
BsaHI 1088 GGCGCC G[GA]^CG[CT]C
BseRI 1814 CTCCTC GAGGAG(10/8)
BsmAI 1557 GAGAC GTCTC(1/5)
BsmBI 1557 GAGACG CGTCTC(1/5)
BsmFI 219 GGGAC GGGAC(10/14)
BsmI 1576 GAATGC GAATGC(1/-1)
BspMI 1476 ACCTGC ACCTGC(4/8)
BsrFI 247 GCCGGC [GA]^CCGG[CT]
BssSI 537 CTCGTG CACGAG(-5/-1)
Bst1107I 2073 GTATAC GTA^TAC
BstUI 1052 CGCG CG^CG
BstZ17I 2073 GTATAC GTA^TAC
CfoI 1089 GCGC GCG^C
Cfr10I 247 GCCGGC [GA]^CCGG[CT]
DraII 1317 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 132 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1317 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1128 CCAAGG C^C[AT][AT]GG
HaeII 1088 GGCGCC [GA]GCGC^[CT]
HapII 248 CCGG C^CGG
HhaI 1089 GCGC GCG^C
Hin1I 1088 GGCGCC G[GA]^CG[CT]C
HinP1I 1089 GCGC G^CGC
HpaII 248 CCGG C^CGG
KasI 1088 GGCGCC G^GCGCC
MspI 248 CCGG C^CGG
MvnI 1052 CGCG CG^CG
NaeI 247 GCCGGC GCC^GGC
NarI 1088 GGCGCC GG^CGCC
NgoMIV 247 GCCGGC G^CCGGC
PflMI 1492 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 719 CACGTG CAC^GTG
PmlI 719 CACGTG CAC^GTG
PpuMI 1317 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1653 CTGCAG CTGCA^G
SapI 815 GAAGAGC GCTCTTC(1/4)
SbfI 1652 CCTGCAGG CCTGCA^GG
SfcI 1653 CTGCAG C^T[GA][CT]AG
SfoI 1088 GGCGCC GGC^GCC
SphI 739 GCATGC GCATG^C
Sse8387I 1652 CCTGCAGG CCTGCA^GG
StyI 1128 CCAAGG C^C[AT][AT]GG
Van91I 1492 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1581 CCATCGCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 235 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1064 GTCTAC GT^[AC][GT]AC
2073 GTATAC
BalI 1098 TGGCCA TGG^CCA
1186 TGGCCA
BanI 1088 GGCGCC G^G[CT][GA]CC
1793 GGTGCC
BanII 818 GAGCTC G[GA]GC[CT]^C
1439 GAGCTC
BglI 1091 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1586 GCCAGCTTGGC
BsaJI 1128 CCAAGG C^C[ACGT][ACGT]GG
1773 CCTGGG
BsgI 1350 CTGCAC GTGCAG(16/14)
1857 CTGCAC
BsrDI 1281 CATTGC GCAATG(2/0)
1639 CATTGC
BstXI 280 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2027 CCACCATACTGG
BstYI 1345 AGATCC [GA]^GATC[CT]
1544 GGATCT
DraI 710 TTTAAA TTT^AAA
2103 TTTAAA
EaeI 1098 TGGCCA [CT]^GGCC[GA]
1186 TGGCCA
EarI 268 GAAGAG CTCTTC(1/4)
815 GAAGAG
HindIII 1241 AAGCTT A^AGCTT
1867 AAGCTT
HphI 227 TCACC GGTGA(8/7)
859 TCACC
Ksp632I 268 GAAGAG CTCTTC(1/4)
815 GAAGAG
MaeII 720 ACGT A^CGT
1560 ACGT
MflI 1345 AGATCC [GA]^GATC[CT]
1544 GGATCT
MluNI 1098 TGGCCA TGG^CCA
1186 TGGCCA
MscI 1098 TGGCCA TGG^CCA
1186 TGGCCA
MslI 1610 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1939 CACATAGGTG
SacI 818 GAGCTC GAGCT^C
1439 GAGCTC
SspI 636 AATATT AAT^ATT
647 AATATT
TaqI 641 TCGA T^CGA
1789 TCGA
TthHB8I 641 TCGA T^CGA
1789 TCGA
XhoII 1345 AGATCC [GA]^GATC[CT]
1544 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI