##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoRV FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1175 GTCTAC GT^[AC][GT]AC
AhdI 431 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 418 CCTCAGG CC^T[ACGT]AGG
ApaI 610 GGGCCC GGGCC^C
Asp700I 304 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 431 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 613 CCCGGG C^[CT]CG[GA]G
AvaII 438 GGTCC G^G[AT]CC
BamHI 513 GGATCC G^GATCC
BanII 610 GGGCCC G[GA]GC[CT]^C
BbvCI 588 CCTCAGC CCTCAGC(-5/-2)
BpmI 780 CTGGAG CTGGAG(16/14)
BsoBI 613 CCCGGG C^[CT]CG[GA]G
BspMI 162 ACCTGC ACCTGC(4/8)
BssSI 334 CTCGTG CACGAG(-5/-1)
BstEII 38 GGTCACC G^GT[ACGT]ACC
BstPI 38 GGTCACC G^GT[ACGT]ACC
Bsu36I 418 CCTCAGG CC^T[ACGT]AGG
Eam1105I 431 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 357 GAAGAG CTCTTC(1/4)
Eco81I 418 CCTCAGG CC^T[ACGT]AGG
EcoO65I 38 GGTCACC G^GT[ACGT]ACC
EcoRI 1100 GAATTC G^AATTC
EcoT14I 696 CCATGG C^C[AT][AT]GG
HapII 614 CCGG C^CGG
HgaI 1173 GCGTC GACGC(5/10)
HincII 1424 GTTGAC GT[CT]^[GA]AC
HindII 1424 GTTGAC GT[CT]^[GA]AC
HpaII 614 CCGG C^CGG
Ksp632I 357 GAAGAG CTCTTC(1/4)
MspI 614 CCGG C^CGG
NcoI 696 CCATGG C^CATGG
NspI 388 ACATGC [GA]CATG^[CT]
SmaI 613 CCCGGG CCC^GGG
SspI 868 AATATT AAT^ATT
StyI 696 CCATGG C^C[AT][AT]GG
XbaI 202 TCTAGA T^CTAGA
XmaCI 613 CCCGGG C^CCGGG
XmaI 613 CCCGGG C^CCGGG
XmnI 304 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1157 CCGC CCGC(-3/-1)
837 GCGG
AfaI 574 GTAC GT^AC
1151 GTAC
AspHI 976 GAGCAC G[AT]GC[AT]^C
1571 GTGCTC
BcnI 613 CCCGG CC^[GC]GG
614 CCGGG
BsiHKAI 976 GAGCAC G[AT]GC[AT]^C
1571 GTGCTC
BsiYI 504 CCCCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
552 CCTGGAAACGG
BslI 504 CCCCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
552 CCTGGAAACGG
BsmFI 715 GGGAC GGGAC(10/14)
962 GTCCC
BsmI 1440 GAATGC GAATGC(1/-1)
1644 GCATTC
BstXI 471 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
965 CCAGGTTTATGG
DraI 1256 TTTAAA TTT^AAA
1311 TTTAAA
DsaI 696 CCATGG C^C[GA][CT]GG
1753 CCACGG
EcoT22I 1563 ATGCAT ATGCA^T
1737 ATGCAT
MaeII 2 ACGT A^CGT
1715 ACGT
MslI 945 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1740 CATTATTGTG
NciI 613 CCCGG CC^[GC]GG
614 CCGGG
NsiI 1563 ATGCAT ATGCA^T
1737 ATGCAT
PleI 548 GACTC GAGTC(4/5)
1704 GAGTC
PstI 349 CTGCAG CTGCA^G
493 CTGCAG
RsaI 574 GTAC GT^AC
1151 GTAC
Tsp45I 39 GTCAC ^GT[GC]AC
1501 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BbvI BfaI BglII BmyI BpuAI
BsaJI BseRI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XhoII