##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 297 TCCGGA T^CCGGA
AcsI 377 GAATTC [GA]^AATT[CT]
AflIII 1254 ACGTGT A^C[GA][CT]GT
AlwNI 126 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1362 AGCGCT AGC^GCT
ApaI 186 GGGCCC GGGCC^C
ApoI 377 GAATTC [GA]^AATT[CT]
Asp700I 1652 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 177 CCCGGG C^[CT]CG[GA]G
BalI 607 TGGCCA TGG^CCA
BanI 934 GGCGCC G^G[CT][GA]CC
BbeI 934 GGCGCC GGCGC^C
BbsI 1439 GTCTTC GAAGAC(2/6)
BpuAI 1439 GTCTTC GAAGAC(2/6)
BsaI 446 GAGACC GGTCTC(1/5)
BseAI 297 TCCGGA T^CCGGA
BsmBI 1506 GAGACG CGTCTC(1/5)
BsoBI 177 CCCGGG C^[CT]CG[GA]G
BspEI 297 TCCGGA T^CCGGA
BsrDI 835 CATTGC GCAATG(2/0)
BsrFI 1290 ACCGGC [GA]^CCGG[CT]
BssSI 81 CACGAG CACGAG(-5/-1)
BstAPI 531 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 509 GGTGACC G^GT[ACGT]ACC
BstPI 509 GGTGACC G^GT[ACGT]ACC
BstXI 1407 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1290 ACCGGC [GA]^CCGG[CT]
DraIII 1497 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1362 AGCGCT AGC^GCT
EcoO65I 509 GGTGACC G^GT[ACGT]ACC
EcoRI 377 GAATTC G^AATTC
KasI 934 GGCGCC G^GCGCC
MluNI 607 TGGCCA TGG^CCA
MroI 297 TCCGGA T^CCGGA
MscI 607 TGGCCA TGG^CCA
NarI 934 GGCGCC GG^CGCC
NspI 808 ACATGC [GA]CATG^[CT]
PflMI 1616 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1166 CAGCTG CAG^CTG
SacI 323 GAGCTC GAGCT^C
SfoI 934 GGCGCC GGC^GCC
SmaI 177 CCCGGG CCC^GGG
Van91I 1616 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 177 CCCGGG C^CCGGG
XmaI 177 CCCGGG C^CCGGG
XmnI 1652 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 31 GACGCC G[GA]^CG[CT]C
934 GGCGCC
AfaI 112 GTAC GT^AC
1312 GTAC
AspHI 69 GTGCTC G[AT]GC[AT]^C
323 GAGCTC
BsaHI 31 GACGCC G[GA]^CG[CT]C
934 GGCGCC
BsaWI 117 TCCGGT [AT]^CCGG[AT]
297 TCCGGA
BsgI 123 GTGCAG GTGCAG(16/14)
923 GTGCAG
BsiHKAI 69 GTGCTC G[AT]GC[AT]^C
323 GAGCTC
BsmFI 1039 GTCCC GGGAC(10/14)
1142 GTCCC
BspMI 642 ACCTGC ACCTGC(4/8)
125 GCAGGT
BsrI 799 CCAGT ACTGG(1/-1)
1270 CCAGT
BstNI 847 CCAGG CC^[AT]GG
852 CCAGG
DraI 1274 TTTAAA TTT^AAA
1558 TTTAAA
EcoNI 138 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
643 CCTGCTGAAGG
EcoRII 847 CCAGG ^CC[AT]GG
852 CCAGG
EcoT14I 604 CCTTGG C^C[AT][AT]GG
1616 CCAAGG
HgaI 31 GACGC GACGC(5/10)
867 GCGTC
Hin1I 31 GACGCC G[GA]^CG[CT]C
934 GGCGCC
HinfI 280 GACTC G^A[ACGT]TC
902 GACTC
HphI 509 GGTGA GGTGA(8/7)
795 TCACC
KspI 40 CCGCGG CCGC^GG
171 CCGCGG
MaeIII 510 GTGAC ^GT[ACGT]AC
1085 GTCAC
MseI 1275 TTAA T^TAA
1559 TTAA
MvaI 847 CCAGG CC^[AT]GG
852 CCAGG
PleI 280 GACTC GAGTC(4/5)
902 GACTC
RsaI 112 GTAC GT^AC
1312 GTAC
SacII 40 CCGCGG CCGC^GG
171 CCGCGG
SapI 756 GCTCTTC GCTCTTC(1/4)
1420 GCTCTTC
StyI 604 CCTTGG C^C[AT][AT]GG
1616 CCAAGG
Tru9I 1275 TTAA T^TAA
1559 TTAA
Tsp45I 510 GTGAC ^GT[GC]AC
1085 GTCAC
Tsp509I 378 AATT ^AATT
1637 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanII BbvI BciVI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI Ksp632I MaeI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I