##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspI        
AviII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglII       BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       CfoI        ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        StuI        SwaI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
AgeI          175  ACCGGT               A^CCGGT
AhdI          468  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          260  GATCC                GGATC(4/5)
AlwNI         392  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          448  CCTTAGG              CC^T[ACGT]AGG
AspEI         468  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         130  GAGCAC               G[AT]GC[AT]^C
AvaI          504  CTCGGG               C^[CT]CG[GA]G
AvrII          40  CCTAGG               C^CTAGG
BanII         146  GAGCCC               G[GA]GC[CT]^C
BcgI          145  CGAGCCCGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          149  CCCGG                CC^[GC]GG
BfaI           41  CTAG                 C^TAG
BglI          115  GCCTGTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           40  CCTAGG               C^CTAGG
BsaI          937  GAGACC               GGTCTC(1/5)
BsiHKAI       130  GAGCAC               G[AT]GC[AT]^C
BsmAI         937  GAGAC                GTCTC(1/5)
BsoBI         504  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1028  TGTACA               T^GTACA
BspMI         216  GCAGGT               ACCTGC(4/8)
BsrGI        1028  TGTACA               T^GTACA
BstUI         109  CGCG                 CG^CG
BstYI         259  AGATCC               [GA]^GATC[CT]
Bsu36I        448  CCTTAGG              CC^T[ACGT]AGG
DraII         112  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      468  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          423  GAAGAG               CTCTTC(1/4)
Eco81I        448  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      112  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         233  GAATTC               G^AATTC
HgaI          860  GCGTC                GACGC(5/10)
HphI         1127  TCACC                GGTGA(8/7)
Ksp632I       423  GAAGAG               CTCTTC(1/4)
MaeI           41  CTAG                 C^TAG
MaeII         700  ACGT                 A^CGT
MaeIII        353  GTTAC                ^GT[ACGT]AC
MflI          259  AGATCC               [GA]^GATC[CT]
MslI         1128  CACCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          109  CGCG                 CG^CG
NaeI           82  GCCGGC               GCC^GGC
NciI          149  CCCGG                CC^[GC]GG
NcoI          785  CCATGG               C^CATGG
NdeI          369  CATATG               CA^TATG
NgoMIV         82  GCCGGC               G^CCGGC
NspI         1063  ACATGC               [GA]CATG^[CT]
PflMI         391  CCAGTTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         175  ACCGGT               A^CCGGT
PshAI        1075  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           97  CTGCAG               CTGCA^G
PvuII         360  CAGCTG               CAG^CTG
SbfI           96  CCTGCAGG             CCTGCA^GG
ScaI          310  AGTACT               AGT^ACT
SmlI          223  CTTGAG               C^T[CT][GA]AG
Sse8387I       96  CCTGCAGG             CCTGCA^GG
SspBI        1028  TGTACA               T^GTACA
SspI          713  AATATT               AAT^ATT
Van91I        391  CCAGTTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1084  CCATTTGCTTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         259  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AseI         1000  ATTAAT               AT^TAAT
             1282  ATTAAT              
AsnI         1000  ATTAAT               AT^TAAT
             1282  ATTAAT              
Asp700I       233  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1201  GAAACAATTC          
BsaWI         175  ACCGGT               [AT]^CCGG[AT]
              208  ACCGGA              
BsmI          408  GCATTC               GAATGC(1/-1)
              986  GCATTC              
BsrDI          10  CATTGC               GCAATG(2/0)
               32  GCAATG              
BsrFI          82  GCCGGC               [GA]^CCGG[CT]
              175  ACCGGT              
Cfr10I         82  GCCGGC               [GA]^CCGG[CT]
              175  ACCGGT              
DsaI          201  CCGTGG               C^C[GA][CT]GG
              785  CCATGG              
EcoT14I        40  CCTAGG               C^C[AT][AT]GG
              785  CCATGG              
MspA1I         15  CAGCGG               C[AC]G^C[GT]G
              360  CAGCTG              
PleI           67  GACTC                GAGTC(4/5)
              226  GAGTC               
PshBI        1000  ATTAAT               AT^TAAT
             1282  ATTAAT              
SfaNI         572  GATGC                GCATC(5/9)
             1124  GCATC               
SfcI           97  CTGCAG               C^T[GA][CT]AG
              357  CTACAG              
StyI           40  CCTAGG               C^C[AT][AT]GG
              785  CCATGG              
XmnI          233  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1201  GAAACAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I