################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspI AviII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 109 CGCG CG^CG AgeI 175 ACCGGT A^CCGGT AhdI 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 260 GATCC GGATC(4/5) AlwNI 392 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 448 CCTTAGG CC^T[ACGT]AGG AspEI 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 130 GAGCAC G[AT]GC[AT]^C AvaI 504 CTCGGG C^[CT]CG[GA]G AvrII 40 CCTAGG C^CTAGG BanII 146 GAGCCC G[GA]GC[CT]^C BcgI 145 CGAGCCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 149 CCCGG CC^[GC]GG BfaI 41 CTAG C^TAG BglI 115 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 40 CCTAGG C^CTAGG BsaI 937 GAGACC GGTCTC(1/5) BsiHKAI 130 GAGCAC G[AT]GC[AT]^C BsmAI 937 GAGAC GTCTC(1/5) BsoBI 504 CTCGGG C^[CT]CG[GA]G Bsp1407I 1028 TGTACA T^GTACA BspMI 216 GCAGGT ACCTGC(4/8) BsrGI 1028 TGTACA T^GTACA BstUI 109 CGCG CG^CG BstYI 259 AGATCC [GA]^GATC[CT] Bsu36I 448 CCTTAGG CC^T[ACGT]AGG DraII 112 GGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 423 GAAGAG CTCTTC(1/4) Eco81I 448 CCTTAGG CC^T[ACGT]AGG EcoO109I 112 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 233 GAATTC G^AATTC HgaI 860 GCGTC GACGC(5/10) HphI 1127 TCACC GGTGA(8/7) Ksp632I 423 GAAGAG CTCTTC(1/4) MaeI 41 CTAG C^TAG MaeII 700 ACGT A^CGT MaeIII 353 GTTAC ^GT[ACGT]AC MflI 259 AGATCC [GA]^GATC[CT] MslI 1128 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 109 CGCG CG^CG NaeI 82 GCCGGC GCC^GGC NciI 149 CCCGG CC^[GC]GG NcoI 785 CCATGG C^CATGG NdeI 369 CATATG CA^TATG NgoMIV 82 GCCGGC G^CCGGC NspI 1063 ACATGC [GA]CATG^[CT] PflMI 391 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 175 ACCGGT A^CCGGT PshAI 1075 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 97 CTGCAG CTGCA^G PvuII 360 CAGCTG CAG^CTG SbfI 96 CCTGCAGG CCTGCA^GG ScaI 310 AGTACT AGT^ACT SmlI 223 CTTGAG C^T[CT][GA]AG Sse8387I 96 CCTGCAGG CCTGCA^GG SspBI 1028 TGTACA T^GTACA SspI 713 AATATT AAT^ATT Van91I 391 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1084 CCATTTGCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 259 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AseI 1000 ATTAAT AT^TAAT 1282 ATTAAT AsnI 1000 ATTAAT AT^TAAT 1282 ATTAAT Asp700I 233 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1201 GAAACAATTC BsaWI 175 ACCGGT [AT]^CCGG[AT] 208 ACCGGA BsmI 408 GCATTC GAATGC(1/-1) 986 GCATTC BsrDI 10 CATTGC GCAATG(2/0) 32 GCAATG BsrFI 82 GCCGGC [GA]^CCGG[CT] 175 ACCGGT Cfr10I 82 GCCGGC [GA]^CCGG[CT] 175 ACCGGT DsaI 201 CCGTGG C^C[GA][CT]GG 785 CCATGG EcoT14I 40 CCTAGG C^C[AT][AT]GG 785 CCATGG MspA1I 15 CAGCGG C[AC]G^C[GT]G 360 CAGCTG PleI 67 GACTC GAGTC(4/5) 226 GAGTC PshBI 1000 ATTAAT AT^TAAT 1282 ATTAAT SfaNI 572 GATGC GCATC(5/9) 1124 GCATC SfcI 97 CTGCAG C^T[GA][CT]AG 357 CTACAG StyI 40 CCTAGG C^C[AT][AT]GG 785 CCATGG XmnI 233 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1201 GAAACAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I