##################################
# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I FauI
FokI FseI FspI HaeII HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 67 GCGG CCGC(-3/-1)
AfaI 719 GTAC GT^AC
AseI 830 ATTAAT AT^TAAT
AsnI 830 ATTAAT AT^TAAT
AvaI 117 CTCGAG C^[CT]CG[GA]G
BanI 758 GGTGCC G^G[CT][GA]CC
BanII 120 GAGCCC G[GA]GC[CT]^C
BclI 370 TGATCA T^GATCA
BmyI 120 GAGCCC G[AGT]GC[ACT]^C
BsmAI 424 GTCTC GTCTC(1/5)
BsmBI 423 CGTCTC CGTCTC(1/5)
BsmFI 274 GTCCC GGGAC(10/14)
BsoBI 117 CTCGAG C^[CT]CG[GA]G
Bsp1286I 120 GAGCCC G[AGT]GC[ACT]^C
BsrDI 663 CATTGC GCAATG(2/0)
Cac8I 222 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 226 GCGC GCG^C
DpnII 371 GATC ^GATC
EcoRI 260 GAATTC G^AATTC
EcoT22I 606 ATGCAT ATGCA^T
FbaI 370 TGATCA T^GATCA
HhaI 226 GCGC GCG^C
HinP1I 226 GCGC G^CGC
MaeII 422 ACGT A^CGT
MboI 371 GATC ^GATC
MwoI 113 GCTGCTCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 371 GATC ^GATC
NsiI 606 ATGCAT ATGCA^T
NspI 353 GCATGT [GA]CATG^[CT]
PacI 504 TTAATTAA TTAAT^TAA
PaeR7I 117 CTCGAG C^TCGAG
PshBI 830 ATTAAT AT^TAAT
RsaI 719 GTAC GT^AC
Sau3AI 371 GATC ^GATC
SmlI 117 CTCGAG C^T[CT][GA]AG
Tsp45I 769 GTCAC ^GT[GC]AC
XbaI 446 TCTAGA T^CTAGA
XcmI 230 CCAACTCCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 117 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbvI 113 GCTGC GCAGC(8/12)
724 GCTGC
BfaI 447 CTAG C^TAG
641 CTAG
BsaJI 10 CCTCGG C^C[ACGT][ACGT]GG
437 CCCTGG
BseRI 36 CTCCTC GAGGAG(10/8)
41 CTCCTC
BssKI 89 CCAGG ^CC[ACGT]GG
438 CCTGG
BstNI 89 CCAGG CC^[AT]GG
438 CCTGG
DraI 522 TTTAAA TTT^AAA
787 TTTAAA
EarI 46 CTCTTC CTCTTC(1/4)
311 GAAGAG
EcoRII 89 CCAGG ^CC[AT]GG
438 CCTGG
Fnu4HI 113 GCTGC GC^[ACGT]GC
724 GCTGC
ItaI 113 GCTGC GC^[ACGT]GC
724 GCTGC
Ksp632I 46 CTCTTC CTCTTC(1/4)
311 GAAGAG
MaeI 447 CTAG C^TAG
641 CTAG
MaeIII 581 GTAAC ^GT[ACGT]AC
769 GTCAC
MslI 374 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
463 CATAAATGTG
MvaI 89 CCAGG CC^[AT]GG
438 CCTGG
NlaIII 61 CATG CATG^
354 CATG
NlaIV 210 GGAACC GG[ACGT]^[ACGT]CC
758 GGTGCC
ScrFI 89 CCAGG CC^[ACGT]GG
438 CCTGG
TseI 113 GCTGC G^C[AT]GC
724 GCTGC
TspRI 82 CAGTG CA[GC]TG[ACGT][ACGT]^
325 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AluI ApoI BsiYI BslI
DdeI HinfI MboII MnlI MseI
TaqI TfiI Tru9I Tsp509I TthHB8I