##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        Aor51HI     AscI        Asp718I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BspEI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       CelII       Cfr10I      
CpoI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoO65I     FauI        FseI        FspI        
HaeII       Hin1I       KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SwaI        
Van91I      


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AhdI         5030  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         2710  ATTAAT               AT^TAAT
AsnI         2710  ATTAAT               AT^TAAT
AspEI        5030  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2489  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI         2582  GCAATGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4718  GTATCC               GTATCC(6/5)
BglI         4939  GCCCGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        3989  ACCGGA               [AT]^CCGG[AT]
BspDI        4738  ATCGAT               AT^CGAT
CfoI         1742  GCGC                 GCG^C
ClaI         4738  ATCGAT               AT^CGAT
Eam1105I     5030  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        5251  GATATC               GAT^ATC
HhaI         1742  GCGC                 GCG^C
HinP1I       1742  GCGC                 G^CGC
HpaI         3280  GTTAAC               GTT^AAC
MaeII        2077  ACGT                 A^CGT
PflFI        2489  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2710  ATTAAT               AT^TAAT
SalI         2768  GTCGAC               G^TCGAC
SbfI         1187  CCTGCAGG             CCTGCA^GG
SmaI         4940  CCCGGG               CCC^GGG
SphI         4289  GCATGC               GCATG^C
Sse8387I     1187  CCTGCAGG             CCTGCA^GG
Tth111I      2489  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        4940  CCCGGG               C^CCGGG
XmaI         4940  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I       3461  GTGCAC               G^TGCAC
             4370  GTGCAC              
AocI         3798  CCTGAGG              CC^T[ACGT]AGG
             4468  CCTGAGG             
ApaI         2841  GGGCCC               GGGCC^C
             5362  GGGCCC              
ApaLI        3461  GTGCAC               G^TGCAC
             4370  GTGCAC              
BalI         3209  TGGCCA               TGG^CCA
             3257  TGGCCA              
BspHI        3182  TCATGA               T^CATGA
             5264  TCATGA              
BsrBI        3341  CCGCTC               CCGCTC(-3/-3)
             5043  CCGCTC              
Bst1107I     1859  GTATAC               GTA^TAC
             6234  GTATAC              
BstZ17I      1859  GTATAC               GTA^TAC
             6234  GTATAC              
Bsu36I       3798  CCTGAGG              CC^T[ACGT]AGG
             4468  CCTGAGG             
DraI         3849  TTTAAA               TTT^AAA
             6208  TTTAAA              
DrdI         1038  GACACCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3639  GACTCAAATGTC        
Eco81I       3798  CCTGAGG              CC^T[ACGT]AGG
             4468  CCTGAGG             
EcoNI        1187  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4749  CCTGAGACAGG         
HincII       2768  GTCGAC               GT[CT]^[GA]AC
             3280  GTTAAC              
HindII       2768  GTCGAC               GT[CT]^[GA]AC
             3280  GTTAAC              
MfeI          240  CAATTG               C^AATTG
             1198  CAATTG              
MluNI        3209  TGGCCA               TGG^CCA
             3257  TGGCCA              
MscI         3209  TGGCCA               TGG^CCA
             3257  TGGCCA              
MunI          240  CAATTG               C^AATTG
             1198  CAATTG              
NcoI         2185  CCATGG               C^CATGG
             2837  CCATGG              
PaeR7I        571  CTCGAG               C^TCGAG
             4294  CTCGAG              
RcaI         3182  TCATGA               T^CATGA
             5264  TCATGA              
ScaI          959  AGTACT               AGT^ACT
             4959  AGTACT              
XbaI         4509  TCTAGA               T^CTAGA
             6339  TCTAGA              
XhoI          571  CTCGAG               C^TCGAG
             4294  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BclI        
BcnI        BfaI        BfrI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRI       EcoRII      EcoT14I     EcoT22I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NheI        NlaIII      NlaIV       NsiI        NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI