##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI AscI Asp718I
AviII AvrII BamHI BbeI BbrPI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI CelII Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII Hin1I KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
Van91I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 5030 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2710 ATTAAT AT^TAAT
AsnI 2710 ATTAAT AT^TAAT
AspEI 5030 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2489 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2582 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4718 GTATCC GTATCC(6/5)
BglI 4939 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3989 ACCGGA [AT]^CCGG[AT]
BspDI 4738 ATCGAT AT^CGAT
CfoI 1742 GCGC GCG^C
ClaI 4738 ATCGAT AT^CGAT
Eam1105I 5030 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5251 GATATC GAT^ATC
HhaI 1742 GCGC GCG^C
HinP1I 1742 GCGC G^CGC
HpaI 3280 GTTAAC GTT^AAC
MaeII 2077 ACGT A^CGT
PflFI 2489 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2710 ATTAAT AT^TAAT
SalI 2768 GTCGAC G^TCGAC
SbfI 1187 CCTGCAGG CCTGCA^GG
SmaI 4940 CCCGGG CCC^GGG
SphI 4289 GCATGC GCATG^C
Sse8387I 1187 CCTGCAGG CCTGCA^GG
Tth111I 2489 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4940 CCCGGG C^CCGGG
XmaI 4940 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 3461 GTGCAC G^TGCAC
4370 GTGCAC
AocI 3798 CCTGAGG CC^T[ACGT]AGG
4468 CCTGAGG
ApaI 2841 GGGCCC GGGCC^C
5362 GGGCCC
ApaLI 3461 GTGCAC G^TGCAC
4370 GTGCAC
BalI 3209 TGGCCA TGG^CCA
3257 TGGCCA
BspHI 3182 TCATGA T^CATGA
5264 TCATGA
BsrBI 3341 CCGCTC CCGCTC(-3/-3)
5043 CCGCTC
Bst1107I 1859 GTATAC GTA^TAC
6234 GTATAC
BstZ17I 1859 GTATAC GTA^TAC
6234 GTATAC
Bsu36I 3798 CCTGAGG CC^T[ACGT]AGG
4468 CCTGAGG
DraI 3849 TTTAAA TTT^AAA
6208 TTTAAA
DrdI 1038 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3639 GACTCAAATGTC
Eco81I 3798 CCTGAGG CC^T[ACGT]AGG
4468 CCTGAGG
EcoNI 1187 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4749 CCTGAGACAGG
HincII 2768 GTCGAC GT[CT]^[GA]AC
3280 GTTAAC
HindII 2768 GTCGAC GT[CT]^[GA]AC
3280 GTTAAC
MfeI 240 CAATTG C^AATTG
1198 CAATTG
MluNI 3209 TGGCCA TGG^CCA
3257 TGGCCA
MscI 3209 TGGCCA TGG^CCA
3257 TGGCCA
MunI 240 CAATTG C^AATTG
1198 CAATTG
NcoI 2185 CCATGG C^CATGG
2837 CCATGG
PaeR7I 571 CTCGAG C^TCGAG
4294 CTCGAG
RcaI 3182 TCATGA T^CATGA
5264 TCATGA
ScaI 959 AGTACT AGT^ACT
4959 AGTACT
XbaI 4509 TCTAGA T^CTAGA
6339 TCTAGA
XhoI 571 CTCGAG C^TCGAG
4294 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI