##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BcgI
BfrI BlnI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 439 GACGTC GACGT^C
AflIII 3504 ACATGT A^C[GA][CT]GT
ApaI 2833 GGGCCC GGGCC^C
AseI 908 ATTAAT AT^TAAT
AsnI 908 ATTAAT AT^TAAT
BalI 3727 TGGCCA TGG^CCA
BanI 2537 GGTGCC G^G[CT][GA]CC
BbvCI 3809 GCTGAGG CCTCAGC(-5/-2)
BglI 2322 GCCATAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3581 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3581 GCTGAGC GC^T[ACGT]AGC
BsaAI 3428 CACGTA [CT]AC^GT[GA]
BsaWI 801 ACCGGA [AT]^CCGG[AT]
BsiEI 101 CGGCCG CG[GA][CT]^CG
BsmI 1987 GCATTC GAATGC(1/-1)
Bsp1407I 2614 TGTACA T^GTACA
BspLU11I 3504 ACATGT A^CATGT
BsrBI 2143 GAGCGG CCGCTC(-3/-3)
BsrDI 207 GCAATG GCAATG(2/0)
BsrGI 2614 TGTACA T^GTACA
BssHII 22 GCGCGC G^CGCGC
CelII 3581 GCTGAGC GC^T[ACGT]AGC
DraIII 991 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 517 GACTTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 101 CGGCCG C^GGCCG
EclXI 101 CGGCCG C^GGCCG
Eco52I 101 CGGCCG C^GGCCG
EcoRV 4158 GATATC GAT^ATC
HaeII 2203 AGCGCC [GA]GCGC^[CT]
HindIII 2390 AAGCTT A^AGCTT
HpaI 1818 GTTAAC GTT^AAC
MluNI 3727 TGGCCA TGG^CCA
MscI 3727 TGGCCA TGG^CCA
NspI 3504 ACATGT [GA]CATG^[CT]
PacI 2369 TTAATTAA TTAAT^TAA
PshAI 2599 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 908 ATTAAT AT^TAAT
SacI 752 GAGCTC GAGCT^C
SalI 274 GTCGAC G^TCGAC
SexAI 2684 ACCAGGT A^CC[AT]GGT
SgrAI 1785 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 3113 CTTGAG C^T[CT][GA]AG
SspBI 2614 TGTACA T^GTACA
SspI 3172 AATATT AAT^ATT
XbaI 823 TCTAGA T^CTAGA
XcmI 3370 CCATATCTTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 132 GTCTAC GT^[AC][GT]AC
274 GTCGAC
AspHI 250 GAGCAC G[AT]GC[AT]^C
752 GAGCTC
AspI 3900 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3965 GACAGGGTC
AvaI 115 CCCGGG C^[CT]CG[GA]G
3833 CCCGGG
BanII 752 GAGCTC G[GA]GC[CT]^C
2833 GGGCCC
BciVI 3441 GGATAC GTATCC(6/5)
1770 GTATCC
BsiHKAI 250 GAGCAC G[AT]GC[AT]^C
752 GAGCTC
BsoBI 115 CCCGGG C^[CT]CG[GA]G
3833 CCCGGG
BspHI 344 TCATGA T^CATGA
3616 TCATGA
BsrFI 1786 GCCGGT [GA]^CCGG[CT]
4033 ACCGGC
BstAPI 1230 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2947 GCACTGGTTGC
Cfr10I 1786 GCCGGT [GA]^CCGG[CT]
4033 ACCGGC
DsaI 32 CCGTGG C^C[GA][CT]GG
2244 CCGTGG
EaeI 101 CGGCCG [CT]^GGCC[GA]
3727 TGGCCA
MfeI 1687 CAATTG C^AATTG
4040 CAATTG
MunI 1687 CAATTG C^AATTG
4040 CAATTG
NruI 168 TCGCGA TCG^CGA
486 TCGCGA
PflFI 3900 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3965 GACAGGGTC
PpuMI 91 GGGTCCT [GA]G^G[AT]CC[CT]
2978 AGGACCC
RcaI 344 TCATGA T^CATGA
3616 TCATGA
ScaI 1393 AGTACT AGT^ACT
3669 AGTACT
SmaI 115 CCCGGG CCC^GGG
3833 CCCGGG
StuI 2066 AGGCCT AGG^CCT
2703 AGGCCT
Tth111I 3900 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3965 GACAGGGTC
XmaCI 115 CCCGGG C^CCGGG
3833 CCCGGG
XmaI 115 CCCGGG C^CCGGG
3833 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII