##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

Acc65I      AccIII      AclI        AflIII      AgeI        
AscI        Asp718I     AviII       BamHI       BcgI        
BglI        BsaWI       BseAI       BsiWI       BspEI       
BspLU11I    BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CpoI        EcoRI       
EcoT22I     FseI        FspI        HindIII     KpnI        
KspI        MfeI        MluI        MroI        MunI        
NdeI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
Psp1406I    RsrII       SacII       SalI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        2536  GACGTC               GACGT^C
AccI            6  GTCTAC               GT^[AC][GT]AC
AhdI         1620  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          195  CCTGAGG              CC^T[ACGT]AGG
AseI         4110  ATTAAT               AT^TAAT
AsnI         4110  ATTAAT               AT^TAAT
AspEI        1620  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1269  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1764  CCTAGG               C^CTAGG
BbeI         1010  GGCGCC               GGCGC^C
BbvCI         978  CCTCAGC              CCTCAGC(-5/-2)
BclI         1135  TGATCA               T^GATCA
BglII         835  AGATCT               A^GATCT
BlnI         1764  CCTAGG               C^CTAGG
BlpI           24  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I       24  GCTAAGC              GC^T[ACGT]AGC
BsaBI        1295  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     4010  TGTACA               T^GTACA
BspDI        1167  ATCGAT               AT^CGAT
BspMI        1060  ACCTGC               ACCTGC(4/8)
BsrGI        4010  TGTACA               T^GTACA
BstAPI       1055  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1783  GGTAACC              G^GT[ACGT]ACC
BstPI        1783  GGTAACC              G^GT[ACGT]ACC
Bsu36I        195  CCTGAGG              CC^T[ACGT]AGG
CelII          24  GCTAAGC              GC^T[ACGT]AGC
ClaI         1167  ATCGAT               AT^CGAT
DrdI         1023  GACGAGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           46  CGGCCG               C^GGCCG
Eam1105I     1620  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          46  CGGCCG               C^GGCCG
Eco52I         46  CGGCCG               C^GGCCG
Eco81I        195  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1783  GGTAACC              G^GT[ACGT]ACC
EcoRV        1113  GATATC               GAT^ATC
FauI           76  GCGGG                CCCGC(4/6)
FbaI         1135  TGATCA               T^GATCA
HincII       2345  GTTAAC               GT[CT]^[GA]AC
HindII       2345  GTTAAC               GT[CT]^[GA]AC
HpaI         2345  GTTAAC               GTT^AAC
KasI         1010  GGCGCC               G^GCGCC
MamI         1295  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1010  GGCGCC               GG^CGCC
NcoI         1015  CCATGG               C^CATGG
PflFI        1269  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        4110  ATTAAT               AT^TAAT
PvuI         1646  CGATCG               CGAT^CG
SapI         2944  GCTCTTC              GCTCTTC(1/4)
ScaI         3114  AGTACT               AGT^ACT
SexAI        2277  ACCTGGT              A^CC[AT]GGT
SfoI         1010  GGCGCC               GGC^GCC
SmaI          499  CCCGGG               CCC^GGG
SspBI        4010  TGTACA               T^GTACA
SspI         3234  AATATT               AAT^ATT
Tth111I      1269  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3365  TCTAGA               T^CTAGA
XmaCI         499  CCCGGG               C^CCGGG
XmaI          499  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI         1010  GGCGCC               G[GA]^CG[CT]C
             2536  GACGTC              
AfaI         3115  GTAC                 GT^AC
             4011  GTAC                
AflII         852  CTTAAG               C^TTAAG
             3791  CTTAAG              
Alw44I        220  GTGCAC               G^TGCAC
             3774  GTGCAC              
Aor51HI       234  AGCGCT               AGC^GCT
              564  AGCGCT              
ApaI         1631  GGGCCC               GGGCC^C
             2013  GGGCCC              
ApaLI         220  GTGCAC               G^TGCAC
             3774  GTGCAC              
Asp700I      3036  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3422  GAACCAATTC          
AvaI          499  CCCGGG               C^[CT]CG[GA]G
             1656  CCCGAG              
BbsI          656  GAAGAC               GAAGAC(2/6)
             3557  GAAGAC              
BciVI         582  GGATAC               GTATCC(6/5)
             1760  GTATCC              
BfrI          852  CTTAAG               C^TTAAG
             3791  CTTAAG              
BpuAI         656  GAAGAC               GAAGAC(2/6)
             3557  GAAGAC              
BsaHI        1010  GGCGCC               G[GA]^CG[CT]C
             2536  GACGTC              
BsiEI          46  CGGCCG               CG[GA][CT]^CG
             1646  CGATCG              
BsmBI        2534  GAGACG               CGTCTC(1/5)
             3891  GAGACG              
BsoBI         499  CCCGGG               C^[CT]CG[GA]G
             1656  CCCGAG              
BspHI         649  TCATGA               T^CATGA
             3946  TCATGA              
BstXI        1549  CCATCATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4346  CCAAAGAGATGG        
DraIII       2276  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2959  CACTAGGTG           
DsaI         1015  CCATGG               C^C[GA][CT]GG
             1682  CCACGG              
Eco47III      234  AGCGCT               AGC^GCT
              564  AGCGCT              
HgaI           15  GACGC                GACGC(5/10)
             3893  GACGC               
Hin1I        1010  GGCGCC               G[GA]^CG[CT]C
             2536  GACGTC              
NaeI           44  GCCGGC               GCC^GGC
             1374  GCCGGC              
NgoMIV         44  GCCGGC               G^CCGGC
             1374  GCCGGC              
NheI         1409  GCTAGC               G^CTAGC
             3533  GCTAGC              
PshAI        3216  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3909  GACTTGAGTC          
RcaI          649  TCATGA               T^CATGA
             3946  TCATGA              
RsaI         3115  GTAC                 GT^AC
             4011  GTAC                
SbfI          248  CCTGCAGG             CCTGCA^GG
             1061  CCTGCAGG            
Sse8387I      248  CCTGCAGG             CCTGCA^GG
             1061  CCTGCAGG            
XcmI          784  CCAAGTCTGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2851  CCAGAAAATGGATGG     
XmnI         3036  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3422  GAACCAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BalI        
BanI        BanII       BbrPI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII