################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AccIII AclI AflIII AgeI AscI Asp718I AviII BamHI BcgI BglI BsaWI BseAI BsiWI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I CpoI EcoRI EcoT22I FseI FspI HindIII KpnI KspI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI Psp1406I RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SphI StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 2536 GACGTC GACGT^C AccI 6 GTCTAC GT^[AC][GT]AC AhdI 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 195 CCTGAGG CC^T[ACGT]AGG AseI 4110 ATTAAT AT^TAAT AsnI 4110 ATTAAT AT^TAAT AspEI 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1764 CCTAGG C^CTAGG BbeI 1010 GGCGCC GGCGC^C BbvCI 978 CCTCAGC CCTCAGC(-5/-2) BclI 1135 TGATCA T^GATCA BglII 835 AGATCT A^GATCT BlnI 1764 CCTAGG C^CTAGG BlpI 24 GCTAAGC GC^T[ACGT]AGC Bpu1102I 24 GCTAAGC GC^T[ACGT]AGC BsaBI 1295 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 4010 TGTACA T^GTACA BspDI 1167 ATCGAT AT^CGAT BspMI 1060 ACCTGC ACCTGC(4/8) BsrGI 4010 TGTACA T^GTACA BstAPI 1055 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1783 GGTAACC G^GT[ACGT]ACC BstPI 1783 GGTAACC G^GT[ACGT]ACC Bsu36I 195 CCTGAGG CC^T[ACGT]AGG CelII 24 GCTAAGC GC^T[ACGT]AGC ClaI 1167 ATCGAT AT^CGAT DrdI 1023 GACGAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 46 CGGCCG C^GGCCG Eam1105I 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 46 CGGCCG C^GGCCG Eco52I 46 CGGCCG C^GGCCG Eco81I 195 CCTGAGG CC^T[ACGT]AGG EcoO65I 1783 GGTAACC G^GT[ACGT]ACC EcoRV 1113 GATATC GAT^ATC FauI 76 GCGGG CCCGC(4/6) FbaI 1135 TGATCA T^GATCA HincII 2345 GTTAAC GT[CT]^[GA]AC HindII 2345 GTTAAC GT[CT]^[GA]AC HpaI 2345 GTTAAC GTT^AAC KasI 1010 GGCGCC G^GCGCC MamI 1295 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1010 GGCGCC GG^CGCC NcoI 1015 CCATGG C^CATGG PflFI 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4110 ATTAAT AT^TAAT PvuI 1646 CGATCG CGAT^CG SapI 2944 GCTCTTC GCTCTTC(1/4) ScaI 3114 AGTACT AGT^ACT SexAI 2277 ACCTGGT A^CC[AT]GGT SfoI 1010 GGCGCC GGC^GCC SmaI 499 CCCGGG CCC^GGG SspBI 4010 TGTACA T^GTACA SspI 3234 AATATT AAT^ATT Tth111I 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3365 TCTAGA T^CTAGA XmaCI 499 CCCGGG C^CCGGG XmaI 499 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 1010 GGCGCC G[GA]^CG[CT]C 2536 GACGTC AfaI 3115 GTAC GT^AC 4011 GTAC AflII 852 CTTAAG C^TTAAG 3791 CTTAAG Alw44I 220 GTGCAC G^TGCAC 3774 GTGCAC Aor51HI 234 AGCGCT AGC^GCT 564 AGCGCT ApaI 1631 GGGCCC GGGCC^C 2013 GGGCCC ApaLI 220 GTGCAC G^TGCAC 3774 GTGCAC Asp700I 3036 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3422 GAACCAATTC AvaI 499 CCCGGG C^[CT]CG[GA]G 1656 CCCGAG BbsI 656 GAAGAC GAAGAC(2/6) 3557 GAAGAC BciVI 582 GGATAC GTATCC(6/5) 1760 GTATCC BfrI 852 CTTAAG C^TTAAG 3791 CTTAAG BpuAI 656 GAAGAC GAAGAC(2/6) 3557 GAAGAC BsaHI 1010 GGCGCC G[GA]^CG[CT]C 2536 GACGTC BsiEI 46 CGGCCG CG[GA][CT]^CG 1646 CGATCG BsmBI 2534 GAGACG CGTCTC(1/5) 3891 GAGACG BsoBI 499 CCCGGG C^[CT]CG[GA]G 1656 CCCGAG BspHI 649 TCATGA T^CATGA 3946 TCATGA BstXI 1549 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4346 CCAAAGAGATGG DraIII 2276 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2959 CACTAGGTG DsaI 1015 CCATGG C^C[GA][CT]GG 1682 CCACGG Eco47III 234 AGCGCT AGC^GCT 564 AGCGCT HgaI 15 GACGC GACGC(5/10) 3893 GACGC Hin1I 1010 GGCGCC G[GA]^CG[CT]C 2536 GACGTC NaeI 44 GCCGGC GCC^GGC 1374 GCCGGC NgoMIV 44 GCCGGC G^CCGGC 1374 GCCGGC NheI 1409 GCTAGC G^CTAGC 3533 GCTAGC PshAI 3216 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3909 GACTTGAGTC RcaI 649 TCATGA T^CATGA 3946 TCATGA RsaI 3115 GTAC GT^AC 4011 GTAC SbfI 248 CCTGCAGG CCTGCA^GG 1061 CCTGCAGG Sse8387I 248 CCTGCAGG CCTGCA^GG 1061 CCTGCAGG XcmI 784 CCAAGTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2851 CCAGAAAATGGATGG XmnI 3036 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3422 GAACCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcsI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII