################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AclI AflII AscI AseI AsnI AspI AviII AvrII BfrI BlnI BsiEI BsiWI BspHI Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI EclXI Eco52I EcoRV FseI FspI HincII HindII HpaI MfeI MluI MunI NdeI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SfiI SfuI SgrAI SpeI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 2496 GGTACC G^GTACC AccI 3464 GTCTAC GT^[AC][GT]AC AccIII 3354 TCCGGA T^CCGGA AgeI 2640 ACCGGT A^CCGGT Aor51HI 1346 AGCGCT AGC^GCT Asp718I 2496 GGTACC G^GTACC BalI 696 TGGCCA TGG^CCA BbeI 477 GGCGCC GGCGC^C BbrPI 1274 CACGTG CAC^GTG BciVI 2967 GTATCC GTATCC(6/5) BglI 1537 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2488 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2488 GCTGAGC GC^T[ACGT]AGC BsaBI 1102 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2015 GAGACC GGTCTC(1/5) BseAI 3354 TCCGGA T^CCGGA BspDI 2029 ATCGAT AT^CGAT BspEI 3354 TCCGGA T^CCGGA BspLU11I 552 ACATGT A^CATGT BstEII 1522 GGTGACC G^GT[ACGT]ACC BstPI 1522 GGTGACC G^GT[ACGT]ACC CelII 2488 GCTGAGC GC^T[ACGT]AGC ClaI 2029 ATCGAT AT^CGAT DraIII 2543 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 696 TGGCCA [CT]^GGCC[GA] Eco47III 1346 AGCGCT AGC^GCT EcoO65I 1522 GGTGACC G^GT[ACGT]ACC EcoRI 3675 GAATTC G^AATTC HindIII 1298 AAGCTT A^AGCTT KasI 477 GGCGCC G^GCGCC KpnI 2496 GGTACC GGTAC^C KspI 38 CCGCGG CCGC^GG MamI 1102 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 696 TGGCCA TGG^CCA MroI 3354 TCCGGA T^CCGGA MscI 696 TGGCCA TGG^CCA NaeI 2808 GCCGGC GCC^GGC NarI 477 GGCGCC GG^CGCC NcoI 1947 CCATGG C^CATGG NgoMIV 2808 GCCGGC G^CCGGC NheI 2170 GCTAGC G^CTAGC PflMI 2005 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2640 ACCGGT A^CCGGT PmaCI 1274 CACGTG CAC^GTG PmlI 1274 CACGTG CAC^GTG SacII 38 CCGCGG CCGC^GG SexAI 2093 ACCAGGT A^CC[AT]GGT SfoI 477 GGCGCC GGC^GCC SmaI 324 CCCGGG CCC^GGG SnaBI 639 TACGTA TAC^GTA SphI 3849 GCATGC GCATG^C SspI 4019 AATATT AAT^ATT StuI 1432 AGGCCT AGG^CCT Van91I 2005 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3973 TCTAGA T^CTAGA XmaCI 324 CCCGGG C^CCGGG XmaI 324 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 552 ACATGT A^C[GA][CT]GT 2547 ACGTGT AhdI 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 506 GACTTCGTGTC Alw44I 1054 GTGCAC G^TGCAC 1304 GTGCAC AocI 404 CCTGAGG CC^T[ACGT]AGG 1528 CCTTAGG ApaI 13 GGGCCC GGGCC^C 1811 GGGCCC ApaLI 1054 GTGCAC G^TGCAC 1304 GTGCAC Asp700I 502 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1832 GAAAAATTTC AspEI 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 506 GACTTCGTGTC AvaI 324 CCCGGG C^[CT]CG[GA]G 1177 CTCGGG BanI 477 GGCGCC G^G[CT][GA]CC 2496 GGTACC BbvCI 3260 CCTCAGC CCTCAGC(-5/-2) 3813 GCTGAGG BglII 2928 AGATCT A^GATCT 4374 AGATCT BsmI 2243 GAATGC GAATGC(1/-1) 3471 GCATTC BsoBI 324 CCCGGG C^[CT]CG[GA]G 1177 CTCGGG Bsp1407I 303 TGTACA T^GTACA 2064 TGTACA BsrBI 627 CCGCTC CCGCTC(-3/-3) 1675 GAGCGG BsrDI 1762 CATTGC GCAATG(2/0) 3399 GCAATG BsrGI 303 TGTACA T^GTACA 2064 TGTACA BssHII 1145 GCGCGC G^CGCGC 1269 GCGCGC BssSI 2948 CACGAG CACGAG(-5/-1) 3488 CACGAG BstAPI 1240 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3820 GCACGCGCTGC BstXI 687 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1815 CCACCATATTGG Bsu36I 404 CCTGAGG CC^T[ACGT]AGG 1528 CCTTAGG Eam1105I 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 506 GACTTCGTGTC Eco81I 404 CCTGAGG CC^T[ACGT]AGG 1528 CCTTAGG SacI 761 GAGCTC GAGCT^C 2591 GAGCTC SapI 758 GAAGAGC GCTCTTC(1/4) 1222 GCTCTTC SbfI 1435 CCTGCAGG CCTGCA^GG 1585 CCTGCAGG ScaI 945 AGTACT AGT^ACT 1320 AGTACT Sse8387I 1435 CCTGCAGG CCTGCA^GG 1585 CCTGCAGG SspBI 303 TGTACA T^GTACA 2064 TGTACA XcmI 2436 CCATAGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3613 CCAGAAACATGATGG XmnI 502 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1832 GAAAAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BamHI BanII BbsI BbvI BcgI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII