##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AclI        AflII       AscI        AseI        
AsnI        AspI        AviII       AvrII       BfrI        
BlnI        BsiEI       BsiWI       BspHI       Bst1107I    
BstBI       BstZ17I     CpoI        DraI        DrdI        
EagI        EclXI       Eco52I      EcoRV       FseI        
FspI        HincII      HindII      HpaI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SfiI        SfuI        SgrAI       
SpeI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       2496  GGTACC               G^GTACC
AccI         3464  GTCTAC               GT^[AC][GT]AC
AccIII       3354  TCCGGA               T^CCGGA
AgeI         2640  ACCGGT               A^CCGGT
Aor51HI      1346  AGCGCT               AGC^GCT
Asp718I      2496  GGTACC               G^GTACC
BalI          696  TGGCCA               TGG^CCA
BbeI          477  GGCGCC               GGCGC^C
BbrPI        1274  CACGTG               CAC^GTG
BciVI        2967  GTATCC               GTATCC(6/5)
BglI         1537  GCCGCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2488  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2488  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1102  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2015  GAGACC               GGTCTC(1/5)
BseAI        3354  TCCGGA               T^CCGGA
BspDI        2029  ATCGAT               AT^CGAT
BspEI        3354  TCCGGA               T^CCGGA
BspLU11I      552  ACATGT               A^CATGT
BstEII       1522  GGTGACC              G^GT[ACGT]ACC
BstPI        1522  GGTGACC              G^GT[ACGT]ACC
CelII        2488  GCTGAGC              GC^T[ACGT]AGC
ClaI         2029  ATCGAT               AT^CGAT
DraIII       2543  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          696  TGGCCA               [CT]^GGCC[GA]
Eco47III     1346  AGCGCT               AGC^GCT
EcoO65I      1522  GGTGACC              G^GT[ACGT]ACC
EcoRI        3675  GAATTC               G^AATTC
HindIII      1298  AAGCTT               A^AGCTT
KasI          477  GGCGCC               G^GCGCC
KpnI         2496  GGTACC               GGTAC^C
KspI           38  CCGCGG               CCGC^GG
MamI         1102  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         696  TGGCCA               TGG^CCA
MroI         3354  TCCGGA               T^CCGGA
MscI          696  TGGCCA               TGG^CCA
NaeI         2808  GCCGGC               GCC^GGC
NarI          477  GGCGCC               GG^CGCC
NcoI         1947  CCATGG               C^CATGG
NgoMIV       2808  GCCGGC               G^CCGGC
NheI         2170  GCTAGC               G^CTAGC
PflMI        2005  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2640  ACCGGT               A^CCGGT
PmaCI        1274  CACGTG               CAC^GTG
PmlI         1274  CACGTG               CAC^GTG
SacII          38  CCGCGG               CCGC^GG
SexAI        2093  ACCAGGT              A^CC[AT]GGT
SfoI          477  GGCGCC               GGC^GCC
SmaI          324  CCCGGG               CCC^GGG
SnaBI         639  TACGTA               TAC^GTA
SphI         3849  GCATGC               GCATG^C
SspI         4019  AATATT               AAT^ATT
StuI         1432  AGGCCT               AGG^CCT
Van91I       2005  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3973  TCTAGA               T^CTAGA
XmaCI         324  CCCGGG               C^CCGGG
XmaI          324  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflIII        552  ACATGT               A^C[GA][CT]GT
             2547  ACGTGT              
AhdI           80  GACGCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              506  GACTTCGTGTC         
Alw44I       1054  GTGCAC               G^TGCAC
             1304  GTGCAC              
AocI          404  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTTAGG             
ApaI           13  GGGCCC               GGGCC^C
             1811  GGGCCC              
ApaLI        1054  GTGCAC               G^TGCAC
             1304  GTGCAC              
Asp700I       502  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1832  GAAAAATTTC          
AspEI          80  GACGCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              506  GACTTCGTGTC         
AvaI          324  CCCGGG               C^[CT]CG[GA]G
             1177  CTCGGG              
BanI          477  GGCGCC               G^G[CT][GA]CC
             2496  GGTACC              
BbvCI        3260  CCTCAGC              CCTCAGC(-5/-2)
             3813  GCTGAGG             
BglII        2928  AGATCT               A^GATCT
             4374  AGATCT              
BsmI         2243  GAATGC               GAATGC(1/-1)
             3471  GCATTC              
BsoBI         324  CCCGGG               C^[CT]CG[GA]G
             1177  CTCGGG              
Bsp1407I      303  TGTACA               T^GTACA
             2064  TGTACA              
BsrBI         627  CCGCTC               CCGCTC(-3/-3)
             1675  GAGCGG              
BsrDI        1762  CATTGC               GCAATG(2/0)
             3399  GCAATG              
BsrGI         303  TGTACA               T^GTACA
             2064  TGTACA              
BssHII       1145  GCGCGC               G^CGCGC
             1269  GCGCGC              
BssSI        2948  CACGAG               CACGAG(-5/-1)
             3488  CACGAG              
BstAPI       1240  GCAGGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3820  GCACGCGCTGC         
BstXI         687  CCATGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCACCATATTGG        
Bsu36I        404  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTTAGG             
Eam1105I       80  GACGCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              506  GACTTCGTGTC         
Eco81I        404  CCTGAGG              CC^T[ACGT]AGG
             1528  CCTTAGG             
SacI          761  GAGCTC               GAGCT^C
             2591  GAGCTC              
SapI          758  GAAGAGC              GCTCTTC(1/4)
             1222  GCTCTTC             
SbfI         1435  CCTGCAGG             CCTGCA^GG
             1585  CCTGCAGG            
ScaI          945  AGTACT               AGT^ACT
             1320  AGTACT              
Sse8387I     1435  CCTGCAGG             CCTGCA^GG
             1585  CCTGCAGG            
SspBI         303  TGTACA               T^GTACA
             2064  TGTACA              
XcmI         2436  CCATAGTTGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3613  CCAGAAACATGATGG     
XmnI          502  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1832  GAAAAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BamHI       BanII       BbsI        BbvI        
BcgI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII