##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
Alw44I ApaLI AscI Asp718I AspI
AviII AvrII BbeI BbrPI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BssHII BssSI BstBI BstEII
BstPI BstUI CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
EcoO65I EcoT22I FauI FspI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacII SalI SfoI SfuI SgrAI
SnaBI SpeI SphI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 1502 GTATAC GT^[AC][GT]AC
AclI 4465 AACGTT AA^CGTT
AcyI 1516 GGCGTC G[GA]^CG[CT]C
AflIII 3916 ACATGT A^C[GA][CT]GT
AhdI 1815 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 32 CCTGAGG CC^T[ACGT]AGG
Aor51HI 338 AGCGCT AGC^GCT
ApaI 4572 GGGCCC GGGCC^C
AseI 3946 ATTAAT AT^TAAT
AsnI 3946 ATTAAT AT^TAAT
AspEI 1815 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 884 TGGCCA TGG^CCA
BamHI 527 GGATCC G^GATCC
BcgI 534 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3880 AGATCT A^GATCT
BsaHI 1516 GGCGTC G[GA]^CG[CT]C
BsmBI 615 GAGACG CGTCTC(1/5)
BspLU11I 3916 ACATGT A^CATGT
BsrBI 4498 GAGCGG CCGCTC(-3/-3)
BsrFI 1446 GCCGGC [GA]^CCGG[CT]
Bst1107I 1502 GTATAC GTA^TAC
BstXI 1535 CCAGCAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1502 GTATAC GTA^TAC
Bsu36I 32 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1446 GCCGGC [GA]^CCGG[CT]
Eam1105I 1815 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 338 AGCGCT AGC^GCT
Eco81I 32 CCTGAGG CC^T[ACGT]AGG
EcoRI 1136 GAATTC G^AATTC
FseI 1445 GGCCGGCC GGCCGG^CC
HaeII 338 AGCGCT [GA]GCGC^[CT]
HgaI 1517 GCGTC GACGC(5/10)
Hin1I 1516 GGCGTC G[GA]^CG[CT]C
HincII 636 GTTAAC GT[CT]^[GA]AC
HindII 636 GTTAAC GT[CT]^[GA]AC
HpaI 636 GTTAAC GTT^AAC
MluNI 884 TGGCCA TGG^CCA
MscI 884 TGGCCA TGG^CCA
NaeI 1446 GCCGGC GCC^GGC
NdeI 880 CATATG CA^TATG
NgoMIV 1446 GCCGGC G^CCGGC
PshAI 2149 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3946 ATTAAT AT^TAAT
Psp1406I 4465 AACGTT AA^CGTT
SapI 2741 GAAGAGC GCTCTTC(1/4)
SbfI 2978 CCTGCAGG CCTGCA^GG
ScaI 961 AGTACT AGT^ACT
SexAI 4326 ACCTGGT A^CC[AT]GGT
SfiI 4564 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4575 CCCGGG CCC^GGG
Sse8387I 2978 CCTGCAGG CCTGCA^GG
XmaCI 4575 CCCGGG C^CCGGG
XmaI 4575 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 1665 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2994 GAACTTCTTC
AvaI 1549 CTCGGG C^[CT]CG[GA]G
4575 CCCGGG
BbvCI 2226 GCTGAGG CCTCAGC(-5/-2)
2469 GCTGAGG
BciVI 306 GTATCC GTATCC(6/5)
3646 GTATCC
BclI 1621 TGATCA T^GATCA
2687 TGATCA
BsaI 920 GAGACC GGTCTC(1/5)
118 GGTCTC
BsoBI 1549 CTCGGG C^[CT]CG[GA]G
4575 CCCGGG
BstAPI 790 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1046 GCAAAGGTTGC
CfoI 339 GCGC GCG^C
2400 GCGC
DsaI 1145 CCATGG C^C[GA][CT]GG
3629 CCATGG
EaeI 884 TGGCCA [CT]^GGCC[GA]
1448 CGGCCA
EcoRV 228 GATATC GAT^ATC
3397 GATATC
FbaI 1621 TGATCA T^GATCA
2687 TGATCA
HhaI 339 GCGC GCG^C
2400 GCGC
HinP1I 339 GCGC G^CGC
2400 GCGC
NcoI 1145 CCATGG C^CATGG
3629 CCATGG
NspI 1903 GCATGT [GA]CATG^[CT]
3916 ACATGT
SspI 3788 AATATT AAT^ATT
4239 AATATT
StuI 36 AGGCCT AGG^CCT
2414 AGGCCT
TaqI 232 TCGA T^CGA
4559 TCGA
TthHB8I 232 TCGA T^CGA
4559 TCGA
XcmI 2365 CCAGCCGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4615 CCACCTTAAGAATGG
XmnI 1665 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2994 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BfrI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII