##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AhdI Alw44I
ApaLI AscI AspEI AspI AvrII
BamHI BfrI BglI BlnI BsiEI
BsiWI BsmI BspDI BspMI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HpaI KspI MluI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1667 GGTACC G^GTACC
AccII 1118 CGCG CG^CG
AccIII 2720 TCCGGA T^CCGGA
AcyI 1390 GGCGCC G[GA]^CG[CT]C
AgeI 1420 ACCGGT A^CCGGT
Aor51HI 536 AGCGCT AGC^GCT
Asp700I 2404 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1667 GGTACC G^GTACC
BbeI 1390 GGCGCC GGCGC^C
BbrPI 2626 CACGTG CAC^GTG
BbvCI 754 GCTGAGG CCTCAGC(-5/-2)
BcgI 2909 GCAAATTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 63 GGATAC GTATCC(6/5)
BsaHI 1390 GGCGCC G[GA]^CG[CT]C
BseAI 2720 TCCGGA T^CCGGA
BspEI 2720 TCCGGA T^CCGGA
BsrBI 778 GAGCGG CCGCTC(-3/-3)
BsrDI 1769 GCAATG GCAATG(2/0)
BstUI 1118 CGCG CG^CG
DrdI 2029 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 536 AGCGCT AGC^GCT
EcoRI 2854 GAATTC G^AATTC
EcoT22I 1981 ATGCAT ATGCA^T
Hin1I 1390 GGCGCC G[GA]^CG[CT]C
HindIII 2675 AAGCTT A^AGCTT
KasI 1390 GGCGCC G^GCGCC
KpnI 1667 GGTACC GGTAC^C
MfeI 1733 CAATTG C^AATTG
MroI 2720 TCCGGA T^CCGGA
MunI 1733 CAATTG C^AATTG
MvnI 1118 CGCG CG^CG
NarI 1390 GGCGCC GG^CGCC
NdeI 3049 CATATG CA^TATG
NsiI 1981 ATGCAT ATGCA^T
PflMI 897 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1420 ACCGGT A^CCGGT
PmaCI 2626 CACGTG CAC^GTG
PmlI 2626 CACGTG CAC^GTG
SapI 2329 GCTCTTC GCTCTTC(1/4)
ScaI 251 AGTACT AGT^ACT
SfoI 1390 GGCGCC GGC^GCC
SgrAI 1419 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2849 CCCGGG CCC^GGG
SphI 792 GCATGC GCATG^C
Van91I 897 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2849 CCCGGG C^CCGGG
XmaI 2849 CCCGGG C^CCGGG
XmnI 2404 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccI 2506 GTCTAC GT^[AC][GT]AC
3610 GTCTAC
AlwNI 815 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1002 CAGCATCTG
AocI 283 CCTGAGG CC^T[ACGT]AGG
3626 CCTCAGG
ApaI 2388 GGGCCC GGGCC^C
3253 GGGCCC
AseI 921 ATTAAT AT^TAAT
1060 ATTAAT
AsnI 921 ATTAAT AT^TAAT
1060 ATTAAT
AvaI 1763 CCCGAG C^[CT]CG[GA]G
2849 CCCGGG
AviII 716 TGCGCA TGC^GCA
1880 TGCGCA
BalI 1661 TGGCCA TGG^CCA
2609 TGGCCA
BfaI 1145 CTAG C^TAG
1246 CTAG
BlpI 41 GCTGAGC GC^T[ACGT]AGC
2125 GCTGAGC
Bpu1102I 41 GCTGAGC GC^T[ACGT]AGC
2125 GCTGAGC
BsaAI 2181 TACGTG [CT]AC^GT[GA]
2626 CACGTG
BsaBI 2010 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2227 GATTGGGATC
BsaWI 1420 ACCGGT [AT]^CCGG[AT]
2720 TCCGGA
BsoBI 1763 CCCGAG C^[CT]CG[GA]G
2849 CCCGGG
Bsp1407I 1502 TGTACA T^GTACA
2663 TGTACA
BspHI 201 TCATGA T^CATGA
2858 TCATGA
BsrFI 842 ACCGGC [GA]^CCGG[CT]
1420 ACCGGT
BsrGI 1502 TGTACA T^GTACA
2663 TGTACA
BstXI 1913 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2285 CCAACATTCTGG
Bsu36I 283 CCTGAGG CC^T[ACGT]AGG
3626 CCTCAGG
CelII 41 GCTGAGC GC^T[ACGT]AGC
2125 GCTGAGC
Cfr10I 842 ACCGGC [GA]^CCGG[CT]
1420 ACCGGT
DraIII 819 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
898 CACATTGTG
Eco81I 283 CCTGAGG CC^T[ACGT]AGG
3626 CCTCAGG
FauI 2870 CCCGC CCCGC(4/6)
2471 GCGGG
FspI 716 TGCGCA TGC^GCA
1880 TGCGCA
HgaI 1116 GACGC GACGC(5/10)
2585 GCGTC
HincII 1843 GTCAAC GT[CT]^[GA]AC
1888 GTCAAC
HindII 1843 GTCAAC GT[CT]^[GA]AC
1888 GTCAAC
MaeI 1145 CTAG C^TAG
1246 CTAG
MamI 2010 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2227 GATTGGGATC
MluNI 1661 TGGCCA TGG^CCA
2609 TGGCCA
MscI 1661 TGGCCA TGG^CCA
2609 TGGCCA
NcoI 477 CCATGG C^CATGG
3308 CCATGG
PpuMI 3466 GGGTCCC [GA]G^G[AT]CC[CT]
3565 GGGACCT
PshAI 618 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3337 GACCTCTGTC
PshBI 921 ATTAAT AT^TAAT
1060 ATTAAT
PvuII 707 CAGCTG CAG^CTG
3443 CAGCTG
RcaI 201 TCATGA T^CATGA
2858 TCATGA
SmlI 2074 CTCAAG C^T[CT][GA]AG
2134 CTTGAG
SspBI 1502 TGTACA T^GTACA
2663 TGTACA
XcmI 2285 CCAACATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3548 CCATGCTGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbsI BbvI BclI BcnI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII